Effects of acute and sub-acute hypobaric hypoxia on oxidative stress: a field study in the Alps

Mrakic‐Sposta, Simona; Gussoni, M.; Dellanoce, Cinzia; Marzorati, Mauro; Montorsi, Michela; Rasica, Letizia; Pratali, Lorenza; D’Angelo, Gennaro; Martinelli, Massimo; Bastiani, Luca; Natale, L.; Vezzoli, Alessandra

doi:10.1007/s00421-020-04527-x

Cited by 23 publications

(36 citation statements)

References 39 publications

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“…Bosco et al [18], who found that D. melanogaster imago's lifespan was strongly affected by hypoxia and hyperbaria, supposed that an induction of oxidative stress might explain this phenomenon. This suggestion is supported by recent human studies on hypobaric hypoxia effect on generation of reactive oxygen species [30,31]. Taking this into account, we think it possible that the drastic decrease in atmospheric pressure that occurred during our March experiment caused hypoxia and oxidative stress resulting in the fertility drop similar to that caused by heat stress.…”

Section: Resultssupporting

confidence: 82%

A Link between Atmospheric Pressure and Fertility of Drosophila Laboratory Strains

Adonyeva

Menshanov

Грунтенко

2021

Insects

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Standardization of conditions under which insects are kept is of great importance when studying their physiology and researchers do their best to maintain it. Nevertheless, sometimes an obvious side effect of some unaccounted factor affecting insects’ reproduction can be revealed even under thoroughly controlled laboratory conditions. We faced such a phenomenon when studying the fertility level in two wild type Drosophila melanogaster strains. For fertility analysis, 50 newly emerged females and 50 males of each strain under study were transferred to fresh medium daily within 10 days. We found out that fertility of both strains was stable on days 2–10 after the oviposition onset in one experiment, while in another one it was significantly decreased during days 5–10. When compared to publicly available meteorological data, these changes in the fertility level demonstrated a strong association with one weather factor: barometric pressure. Thus, we conclude that changes in atmospheric pressure can be considered a factor affecting insects reproduction and discuss a possible mechanism of their influence on fertility.

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Section: Resultssupporting

confidence: 82%

A Link between Atmospheric Pressure and Fertility of Drosophila Laboratory Strains

Adonyeva

Menshanov

Грунтенко

2021

Insects

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“…EPR, X-band instrument (E-Scan—Bruker BioSpin, GmbH, Ettlingen, Germany) was adopted. ROS production rate was determined by means of a well-consolidated method in blood [ 31 , 32 , 33 , 34 ]. Spin probe, CMH (1-hydroxy-3-methox-ycarbonyl-2,2,5,5-tetramethylpyrrolidine) was used for determination, and stable radical CP· (3-Carboxy2,2,5,5-tetramethyl-1-pyrrolidinyloxy) was used as external reference to convert ROS determinations in absolute quantitative values (μmol·min −1 ).…”

Section: Methodsmentioning

confidence: 99%

Full-Face Mask Use during SCUBA Diving Counters Related Oxidative Stress and Endothelial Dysfunction

Lévénez

Lambrechts

Mrakic‐Sposta

et al. 2022

IJERPH

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Impaired flow mediated dilation (FMD), an index of vascular stress, is known after SCUBA diving. This is related to a dysfunction of nitric oxide (NO) availability and a disturbance of the redox status, possibly induced by hyperoxic/hyperbaric gas breathing. SCUBA diving is usually performed with a mask only covering “half face” (HF) and therefore forcing oral breathing. Nasal NO production is involved in vascular homeostasis and, as consequence, can significantly reduce NO possibly promoting vascular dysfunction. More recently, the utilization of “full-face” (FF) mask, allowing nasal breathing, became more frequent, but no reports are available describing their effects on vascular functions in comparison with HF masks. In this study we assessed and compared the effects of a standard shallow dive (20 min at 10 m) wearing either FF or a HF mask on different markers of vascular function (FMD), oxidative stress (ROS, 8-iso-PGF2α) and NO availability and metabolism (NO2, NOx and 3-NT and iNOS expression). Data from a dive breathing a hypoxic (16% O2 at depth) gas mixture with HF mask are shown allowing hyperoxic/hypoxic exposure. Our data suggest that nasal breathing might significantly reduce the occurrence of vascular dysfunction possibly due to better maintenance of NO production and bioavailability, resulting in a better ability to counter reactive oxygen and nitrogen species. Besides the obvious outcomes in terms of SCUBA diving safety, our data permit a better understanding of the effects of oxygen concentrations, either in normal conditions or as a strategy to induce selected responses in health and disease.

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“…In the same vein, redox homeostasis has been widely studied as disrupted by hypoxia conditioning [ 6 ]. Hypobaric hypoxia negatively affects redox homeostasis, leading to the generation of reactive species and consequently to damage of cellular compartments (i.e., lipids and proteins) and the degeneration of antioxidant systems [ 7 , 8 ]. On the other hand, ascent to high altitudes can lead to an excessive inflammatory response [ 7 , 9 , 10 ] with increased levels of proinflammatory cytokines [ 7 , 9 , 10 ].…”

Section: Introductionmentioning

confidence: 99%

“…Hypobaric hypoxia negatively affects redox homeostasis, leading to the generation of reactive species and consequently to damage of cellular compartments (i.e., lipids and proteins) and the degeneration of antioxidant systems [ 7 , 8 ]. On the other hand, ascent to high altitudes can lead to an excessive inflammatory response [ 7 , 9 , 10 ] with increased levels of proinflammatory cytokines [ 7 , 9 , 10 ]. The reciprocal interplay between oxidative stress and inflammation has been termed as OxInflammation, and it is a source of biomarkers for monitoring the pre-pathological conditions triggered by environmental stressors [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

OxInflammation at High Altitudes: A Proof of Concept from the Himalayas

et al. 2022

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High-altitude locations are fascinating for investigating biological and physiological responses in humans. In this work, we studied the high-altitude response in the plasma and urine of six healthy adult trekkers, who participated in a trek in Nepal that covered 300 km in 19 days along a route in the Kanchenjunga Mountain and up to a maximum altitude of 5140 m. Post-trek results showed an unbalance in redox status, with an upregulation of ROS (+19%), NOx (+28%), neopterin (+50%), and pro-inflammatory prostanoids, such as PGE2 (+120%) and 15-deoxy-delta12,14-PGJ2 (+233%). The isoprostane 15-F2t-IsoP was associated with low levels of TAC (−18%), amino-thiols, omega-3 PUFAs, and anti-inflammatory CYP450 EPA-derived mediators, such as DiHETEs. The deterioration of antioxidant systems paves the way to the overload of redox and inflammative markers, as triggered by the combined physical and hypoxic stressors. Our data underline the link between oxidative stress and inflammation, which is related to the concept of OxInflammation into the altitude hypoxia fashion.

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Effects of acute and sub-acute hypobaric hypoxia on oxidative stress: a field study in the Alps

Cited by 23 publications

References 39 publications

A Link between Atmospheric Pressure and Fertility of Drosophila Laboratory Strains

A Link between Atmospheric Pressure and Fertility of Drosophila Laboratory Strains

Full-Face Mask Use during SCUBA Diving Counters Related Oxidative Stress and Endothelial Dysfunction

OxInflammation at High Altitudes: A Proof of Concept from the Himalayas

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