Effects of Acute and Chronic Boysenberry Intake on Blood Pressure and Endothelial Function in Spontaneous Hypertensive Rats

Matsushima, Akito; Furuuchi, Ryo; Shirai, Marie; Nagai, Satoshi; Yokoyama, Tadayuki; Nishida, Hiroshi; Hirayama, Masao

doi:10.3177/jnsv.60.43

Cited by 5 publications

(5 citation statements)

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“…Boysenberry juice has a high content of AC. Intake of boysenberry juice was reported to reduce the blood pressure in spontaneously hypertensive rats (SHR) [ 23 ] and improve FMD in humans [ 24 ]. In the aortas of our mice with dietary obesity and in HUVECs, BP attenuated ROS production by ECs and also inhibited p53 expression.…”

Section: Discussionmentioning

confidence: 99%

“…Boysenberries, also described as hybrid Rubus berry of Rubus baileyanus and Rubus loganobaccus , are an abundant source of polyphenols that may have various physiological effects. In fact, boysenberry juice was reported to lower the blood pressure of spontaneously hypertensive rats [ 23 ], and improve a flow-mediated dilation (FMD) of the brachial artery [ 24 ]. There are four types of polyphenols, including phenolic acids, flavonoids, stilbenes, and lignans.…”

Section: Introductionmentioning

confidence: 99%

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Boysenberry polyphenol inhibits endothelial dysfunction and improves vascular health

et al. 2018

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Endothelial cells have an important role in maintaining vascular homeostasis. Age-related disorders (including obesity, diabetes, and hypertension) or aging per se induce endothelial dysfunction that predisposes to the development of atherosclerosis. Polyphenols have been reported to suppress age-related endothelial cell disorders, but their role in vascular function is yet to be determined. We investigated the influence of boysenberry polyphenol on vascular health under metabolic stress in a murine model of dietary obesity. We found that administration of boysenberry polyphenol suppressed production of reactive oxygen species (ROS) and increased production of nitric oxide (NO) in the aorta. It has been reported that p53 induces cellular senescence and has a crucial role in age-related disorders, including heart failure and diabetes. Administration of boysenberry polyphenol significantly reduced the endothelial p53 level in the aorta and ameliorated endothelial cell dysfunction in iliac arteries under metabolic stress. Boysenberry polyphenol also reduced ROS and p53 levels in cultured human umbilical vein endothelial cells (HUVECs), while increasing NO production. Uncoupled endothelial nitric oxide synthase (eNOS monomer) is known to promote ROS production. We found that boysenberry polyphenol reduced eNOS monomer levels both in vivo and in vitro, along with an increase of eNOS dimerization. To investigate the components of boysenberry polyphenol mediating these favorable biological effects, we extracted the anthocyanin fractions. We found that anthocyanins contributed to suppression of ROS and p53, in association with increased NO production and eNOS dimerization. In an ex vivo study, anthocyanins promoted relaxation of iliac arteries from mice with dietary obesity. These findings indicate that boysenberry polyphenol and anthocyanins, a major component of this polyphenol, inhibit endothelial dysfunction and contribute to maintenance of vascular homeostasis.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Boysenberry polyphenol inhibits endothelial dysfunction and improves vascular health

et al. 2018

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“…Plasma renin activity was measured using the 5-FAM/QXL ® 520 fluorescence resonance energy transfer (FRET) peptide 52 . Reactions were conducted in 96 well microtiter plates containing 40 μL of plasma and the product formation was determined at 37 °C by following the fluorescence as a function of time using a fluorescence plate reader (FLUOstar Omega) at an excitation wavelength of 490 nm and an emission wavelength of 520 nm.…”

Section: Methodsmentioning

confidence: 99%

Role of the Renin Angiotensin System in Blood Pressure Allostasis-induced by Severe Food Restriction in Female Fischer rats

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Abreu

et al. 2018

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Severe food restriction (FR) is associated with blood pressure (BP) and cardiovascular dysfunction. The renin-angiotensin system (RAS) regulates BP and its dysregulation contributes to impaired cardiovascular function. Female Fischer rats were maintained on a control (CT) or severe FR (40% of CT) diet for 14 days. In response to severe FR, BP allostasis was achieved by up-regulating circulating Ang-[1–8] by 1.3-fold through increased angiotensin converting enzyme (ACE) activity and by increasing the expression of AT1Rs 1.7-fold in mesenteric vessels. Activation of the RAS countered the depressor effect of the severe plasma volume reduction (≥30%). The RAS, however, still underperformed as evidenced by reduced pressor responses to Ang-[1–8] even though AT1Rs were still responsive to the depressor effects of an AT1R antagonist. The aldosterone (ALDO) response was also inadequate as no changes in plasma ALDO were observed after the large fall in plasma volume. These findings have implications for individuals who have experienced a period(s) of severe FR (e.g., anorexia nervosa, dieters, natural disasters) and suggests increased activity of the RAS in order to achieve allostasis contributes to the cardiovascular dysfunction associated with inadequate food intake.

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“…Boysenberry, the source of BoyP, is a berry rich in polyphenols, mainly anthocyanins, ellagic acid, and ellagitannins ( Furuuchi et al., 2011 ). BoyP were reported to decrease the level of reactive oxygen species and have a protective effect on vascular endothelial function ( Furuuchi et al., 2018 ; Matsushima et al., 2014 ).…”

Section: Discussionmentioning

confidence: 99%

Endothelial SIRT-1 has a critical role in the maintenance of capillarization in brown adipose tissue

et al. 2022

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Effects of Acute and Chronic Boysenberry Intake on Blood Pressure and Endothelial Function in Spontaneous Hypertensive Rats

Cited by 5 publications

References 21 publications

Boysenberry polyphenol inhibits endothelial dysfunction and improves vascular health

Boysenberry polyphenol inhibits endothelial dysfunction and improves vascular health

Role of the Renin Angiotensin System in Blood Pressure Allostasis-induced by Severe Food Restriction in Female Fischer rats

Endothelial SIRT-1 has a critical role in the maintenance of capillarization in brown adipose tissue

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