Effects of activation and assisted reproduction techniques on the composition, structure, and properties of the sauger (Sander Canadensis) spermatozoa plasma membrane

Blawut, Bryan; Wolfe, Barbara A.; Premanandan, Christopher; Schuenemann, G.M.; Ludsin, Stuart A.; Liu, Shan-Lu; Veeramachaneni, D. N. Rao; Silva, Marco A. Coutinho da

doi:10.1016/j.theriogenology.2022.12.021

Cited by 1 publication

(1 citation statement)

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“…In addition to the restriction of the present account to small-molecule probes, certain other limitations should be pointed out. The model does not address PM derivatives such as exosomes and endosomes, nor does it allow for the fact that PM is sometimes relatively fluid (e.g., in spermatozoa [12]) and can vary in permeability (e.g., during the cell cycle [13]). Finally, it must be acknowledged that reliable estimates of the necessary structure parameters (AI, HGH, and log P, see below) cannot be made for certain types of chemical structures.…”

Section: Introductionmentioning

confidence: 99%

How to Target Small-Molecule Fluorescent Imaging Probes to the Plasma Membrane—The Influence and QSAR Modelling of Amphiphilicity, Lipophilicity, and Flip-Flop

Horobin,

Stockert

2023

Molecules

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Many new fluorescent probes targeting the plasma membrane (PM) of living cells are currently being described. Such probes are carefully designed to report on relevant membrane features, but oddly, the structural features required for effective and selective targeting of PM often receive less attention, constituting a lacuna in the molecular design process. We aim to rectify this by clarifying how the amphiphilicity and lipophilicity of a probe, together with the tendency to flip-flop across the membrane, contribute to selective PM accumulation. A simplistic decision-rule QSAR model has been devised that predicts the accumulation/non-accumulation of small-molecule fluorescent probes in the PM. The model was based on probe log P plus various derived measures, allowing the roles of amphiphilicity, lipophilicity, and flip-flop to be taken into account. The validity and wide applicability of the model were demonstrated by evaluating its ability to predict amphiphilicity or PM accumulation patterns in surfactants, drugs, saponins, and PM probes. It is hoped that the model will aid in the more efficient design of effective PM probes.

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Section: Introductionmentioning

confidence: 99%