Effects of acteoside on the expressions of MCP-1 and TGF-β₁ in the diabetic nephropathy mice

Abstract

Objective: Immune inflammatory cells and cytokines play an important role in the occurrence and development of diabetic nephropathy (DN). Acteoside has been reported to regulate the inflammation and immune response. The study aims to investigate the effects of acteoside on the expressions of MCP-1 and TGF-β1 on nephropathy in diabetic mice. Methods: C57BL/6J mice in the model group were given a single intraperitoneal injection of STZ (150 mg/kg). Model mice were divided randomly into two groups: 5 without treatment, 5 treated with acteoside. After continuous administration for 8 weeks, serum, urine, and kidney tissue were collected, then, ralated biochemical parameters, pathological characteristics and MCP-1 and TGF-β1 mRNA or protein were detected. The NRK-52E cells were divided into three groups as follows: the normal control group (NC group), the high glucose group (HS group), the high glucose+acteoside group (HS+ACT group). The expressions of MCP-1 and TGF-β1 in the mRNA and protein levels were assessed with RT-PCR, western blot and ELISA. Results: The expressions of MCP-1 and TGF-β1 were significantly enhanced in DN group and dramatically reduced after acteoside treatment. Compared with those in NC group, the expressions of MCP-1 and TGF-β1 in NRK-52E cell of HS group were significantly enhanced, while both were significantly decreased in HS+ACT group compared with HS group. Conclusion: Our findings indicate that Acteoside has protective effects on DN via inhibiting the expressions of MCP-1 and TGF-β1.

Both sexes develop DKD in the CD1 uninephrectomized streptozotocin mouse model

“…Our data show significant increases in the number of infiltrating T cells and macrophages in male and female diabetic mice. Increased expression of the macrophage-recruiting chemokine MCP-1 at the transcript level was also seen in both male and female diabetic mice 31 , although higher levels were seen in males. Interestingly, we did not observe any difference in diabetic kidneys in the transcript expression of RANTES, the chemokine known to recruit T cells in DKD.…”

The roles of gut microbiota and its metabolites in diabetic nephropathy

“…IL-6 can act on interstitial cells near capillaries and release collagenase and other extracellular proteases, which leads to the degradation and leakage of glucoproteinases and the occurrence of microalbuminuria, thus promoting the occurrence and development of DN ( Mansoor et al, 2022 ). Chemokines, such as monocyte chemoattractant protein 1 (MCP-1) can cause glomerular basement membrane injury and stimulate TGF-β1 to participate in glomerular sclerosis and tubulointerstitial fibrosis ( Xie et al, 2022 ). Adhesion molecules, such as intracellular adhesion molecule 1 (ICAM-1) can aggregate inflammatory cells through kinase reaction and cause inflammatory response.…”