Effects of acrylamide on oxidant/antioxidant parameters and CYP2E1 expression in rat pancreatic endocrine cells

Marković, Jelena; Stošić, Milena; Kojić, Danijela; Matavulj, M.

doi:10.1016/j.acthis.2017.12.001

Cited by 28 publications

(56 citation statements)

References 52 publications

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“…We demonstrated stronger adverse effects induced by ACR on the SH groups, which may result from the presence of multiple binding with electrophilic properties in the ACR molecule, which determines its greater reactivity with SH groups of proteins [51,52]. The increase in intensity of OS in the presence of ACR is also associated with an increase in the reactive oxygen species, and a decrease in the activity of superoxide dismutase and the concentration of reduced glutathione [20,53]. On the other hand, it is reported that nitrates may also have a positive influence on human health as a source of nitric oxide, which is beneficial for maintaining endothelial functions, and performs a useful protective role in the prevention of cardiovascular diseases [54,55].…”

Section: Discussionmentioning

confidence: 81%

In vivo and ex vivo impact of nutritional xenobiotics – acrylamide and sodium nitrates – on plasma antioxidant properties

Rorbach-Dolata¹,

Żurawska-Płaksej²,

Marchewka³

et al. 2019

Ann. Acad. Med. Siles.

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INTRODUCTION: The thiol (SH) groups present in human blood plasma play an important role in the oxidative/antioxidative homeostasis of the organism. They are susceptible to the adverse actions of different exo-and endogenous factors. Chronic exposure to different xenobiotics, e.g. nitrogen-containing compounds commonly occurring in food, is especially important. The aim of this study was to investigate the effect of acrylamide (ACR) and sodium nitrates (SN)-(V) and (III)-on the plasma antioxidant properties, as reflected by changes in the SH group levels. MATERIAL AND METHODS: The concentration of SH groups was measured by Ellman's method in blood plasma derived from 62 young people (in vivo model; time t0), and after 1 hour of blood plasma incubation with appropriate ACR and SN (III) concentrations (ex vivo model; time t1). The concentrations used corresponded with their daily intake (DIA-daily intake of acrylamide, and DIN-daily intake of sodium nitrates (V) and (III), respectively), estimated on the basis of a nutritional questionnaire. RESULTS: In both models, acrylamide and nitrates caused a significant decrease in SH group concentrations, but ACR induced stronger changes. The women consumed a greater amount of these nitrogen-containing compounds compared to the men, probably due to their different dietary habits. CONCLUSIONS: The obtained results indicate that these nitrogen-containing xenobiotics are important agents lowering antioxidative plasma potential, hence their intake should be controlled. KEY WORDS acrylamide, sodium nitrate (III) and (V), daily intake, thiol groups, antioxidant potential STRESZCZENIE WSTĘP: Grupy tiolowe (SH) obecne w osoczu krwi odgrywają ważną rolę w oksydacyjno-antyoksydacyjnej homeostazie organizmu. Są one podatne na niekorzystne działanie różnych czynników egzo-i endogennych. Szczególnie istotnym problemem jest długotrwałe narażenie na różne ksenobiotyki, np. związki zawierające azot, powszechnie występujące w żywności. Celem naszych badań była ocena wpływu akrylamidu (acrylamide-ACR) i azotanu sodu (sodium nitrate-SN)-(V) i (III)-na właściwości przeciwutleniające osocza poprzez pomiar stężenia grup SH.

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Section: Discussionmentioning

confidence: 81%

In vivo and ex vivo impact of nutritional xenobiotics – acrylamide and sodium nitrates – on plasma antioxidant properties

Rorbach-Dolata¹,

Żurawska-Płaksej²,

Marchewka³

et al. 2019

Ann. Acad. Med. Siles.

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“…Moreover, iNOS further stimulated NF-κB expression, forming a vicious circle and aggravating the process of liver injury. In addition to NF-κB, iNOS could also directly act on the iron-sulfur reaction center of CYP2E1 and destroy the protein, which may be a reason for its downregulated expression [29]. Furthermore, iNOS is an important molecular source of nitration in CYP2E1 posttranslational protein modification [20, 24], explaining why the decreased metabolic activity in immune liver injury was greater than the protein expression.…”

Section: Discussionmentioning

confidence: 99%

NF-κB-mediated regulation of rat CYP2E1 by two independent signaling pathways

Kang

et al. 2019

PLoS ONE

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Cytochrome P450 2E1 (CYP2E1) plays an important role in both alcohol-induced and immune-mediated liver injury. However, the mechanism underlying CYP2E1 transcriptional regulation has not been clarified. This study focused on the NF-κB-mediated transcriptional regulation of rat CYP2E1 by two independent signaling pathways in alcohol-induced and immune-mediated liver injury rat models. Male Sprague-Dawley rats were used in pharmacokinetic, molecular pharmacology, and morphology experiments. A rat model of alcohol-induced liver injury (AL) was established by feeding an ethanol-containing diet (42 g/kg/day) for 5 weeks as indicated. A rat immune-mediated liver injury (IM) model was established by the sequential injection of bacillus Calmette-Guérin (BCG, 125 mg/kg, once) via the tail vein after test day 21 and 10 μg/kg LPS 13 days later. HPLC, real-time PCR, western blot and ELISA analyses were performed. CYP2E1 expression was enhanced during the process of alcohol-induced liver injury (increased by 56%, P < 0.05) and significantly reduced during that of immune-mediated liver injury (reducedby52%, P < 0.05). NF-κB was activated in both the AL and IM groups (increased by 56% and76%, respectively, P < 0.05). Compared to those in the livers of AL model rats, the interleukin (IL)-1β, tumor necrosis factor (TNF)-α, and iNOS levels in IM model rat livers were increased (increased by 26%, 21% and 101%, respectively, P < 0.05). The differential changes in CYP2E1 in the processes of alcohol-induced and immune-mediated liver injury may result from the differential expression of inflammatory cytokines and iNOS after NF-κB activation, leading to the NF-κB-mediated transcriptional regulation of rat CYP2E1 by two independent signaling pathways.

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“…In neural system, AA is a soft electrophile that could form adducts with nucleophilic sulfhydryl groups on cysteine residues of kelch‐like erythroid cell‐derived protein with CNS homology‐associated protein 1 (Keap1) leading to dissociation of the transcription factor, nuclear factor erythroid 2‐related factor 2 (Nrf2), which belongs to the antioxidant‐responsive element (Zhang, Gavin, Barber, & LoPachin, 2011). In mice hepar and rat pancreatic endocrine cells, AA treatment could alter the expression of enzyme cytochrome P450 2E1 (CYP2E1) (Markovic, Stosic, Kojic, & Matavulj, 2018; Zhao et al., 2015). Our previous study also displayed that AA could induce the toxicity through the oxidant damage in brain of rats (Zhao, Wang, Hu, Chen, & Chan, 2017).…”

Section: Introductionmentioning

confidence: 99%

Acrylamide alters the miRNA profiles and miR‐27a‐5p plays the key role in multiple tissues of rats

Zhang

Yang

et al. 2020

Food Frontiers

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Acrylamide (AA) is the potential carcinogen, which can induce multiple toxic effects in laboratory animals and humans. So far, increasing attention have been paid to toxical mechanism and intervention measures of AA, however, these details and methods are still obscure. MicroRNAs (miRNAs) have been demonstrated to be involved in toxical functional mechanisms induced by chemicals in vivo or vitro. To explore novel target and mechanism of AA toxicity, a more detailed miRNA expression profiling study is needed. In this study, we established the short‐term high‐dose model of rats with the treatment of 35 mg/kg·b.w./day AA for 17 days, analyzed the miRNAs expression profiling in AA and control groups, and discovered the altered miRNA profiles in 11 tissues with AA treatment. Interestingly enough, the expression of miR‐27a‐5p were significantly up‐regulated in AA group, especially in bladder and hepar. Furthermore, we found miR‐27a‐5p increased in 11 tissues of long‐term low‐dose AA‐treated rats (3.5 mg/kg·b.w./day for 68 days). Therefore, these results revealed that AA changed miRNAs profiles in multiple tissues of SD rats for the first time and suggested that miR‐27a‐5p could be used as the target biomarker for the detection and interference of AA toxicity.

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Effects of acrylamide on oxidant/antioxidant parameters and CYP2E1 expression in rat pancreatic endocrine cells

Cited by 28 publications

References 52 publications

In vivo and ex vivo impact of nutritional xenobiotics – acrylamide and sodium nitrates – on plasma antioxidant properties

In vivo and ex vivo impact of nutritional xenobiotics – acrylamide and sodium nitrates – on plasma antioxidant properties

NF-κB-mediated regulation of rat CYP2E1 by two independent signaling pathways

Acrylamide alters the miRNA profiles and miR‐27a‐5p plays the key role in multiple tissues of rats

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