Effects of acidic sodium fluoride-treated, commercially pure titanium on periodontal pathogens and rat bone marrow cells

Abstract

The purpose of the present study was to clarify the potentiality of acidic fluoride solution in treating peri-implantitis. We examined bactericidal activity of fluoride solution against periodontal pathogens; and evaluated the effects of fluoride on titanium, and the effects on cell proliferation and differentiation of rat bone marrow cells on the fluoride-treated titanium. Aggregatibacter actinomycetemcomitans and Porphyromonas gingivalis were used to determine minimal inhibitory concentration (MIC) and shorttime exposure. The cells were seeded on the titanium surface with or without fluoride treatment. Then, cellular proliferation, differentiation and mineral deposition were analyzed. The MIC values for A. actinomycetemcomitans and P. gingivalis were 225 and 900 ppm F − , respectively. In short-time exposure test, both bacterial strains exhibited a significant decrease in a concentrationdependent manner. Cell proliferation and mineral deposition were significantly increased on the fluoride-treated surface. Within the limitation of this study, acidic sodium fluoride solution has the potentiality in treating peri-implantitis.

Real-time monitoring of the metabolic activity of periodontopathic bacteria

“…F showed no or a slight inhibitory effect on the tryptone metabolism by Pg, Pi and Fn, as reported previously for Pg (Shimogishi et al, 2014). F is known to inhibit enolase, a glycolytic enzyme, as well as the sugar: phosphoenolpyruvate phosphotransferase system, an enzymatic system for sugar uptake in Sm (Kanapka and Hamilton, 1971).…”

“…The minimum inhibitory concentrations (MICs) on growth of these substances have been reported previously (Amorim et al, 2004;McBain et al, 2004;Shimogishi et al, 2014;Sreenivasan et al, 2013); however, their effects on the metabolic activity of periodontopathic bacteria are still unknown. Therefore, we also attempted to apply our new method to evaluating the effects of these antimicrobial substances found in oral care products on bacterial metabolic activity.…”

Fluoride incorporation in high phosphate containing bioactive glasses and in vitro osteogenic, angiogenic and antibacterial effects

“…A. actinomycetemcomitans is mainly detected in aggressive periodontitis, a severe and rapidly progressing form which most often starts at an early age [90]. In this study, the growth of both bacteria was significantly [91]. A 6-month clinical study found that the fluoride containing dentifrice showed significant reductions in the number of anaerobic bacteria on both the dental implants and control teeth at 3 months [92].…”