1974
DOI: 10.1111/j.1471-4159.1974.tb06949.x
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Effects of acetylcholine on incorporation of [14C]glucose into phosphatidylinositol and on phosphatidylinositol breakdown in subcellular fractions from cerebral cortex

Abstract: cional de Investigaciones Cientificas y Tecnicas de la Republica Argentina and the Wellcome Trust for financial 286 Short communication . -Aires. Paraguay 2155, Buenos A i m . 23, Argentina. Biochim. biophys. Acta 318, 306-312.

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Cited by 14 publications
(8 citation statements)
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“…However, on the basis of data for the release of unesterified fatty acids in rat brain post mortem (Bazan, 1970) and from incubated cerebral-cortex synaptosomal fractions (Price & Rowe, 1972), the higher rate of incorporation of oleate than of arachidonate into the D fraction phospholipid (Table 1) (Holub et al, 1970;Baker & Thompson, 1972;Breckenridge et al, 1973). However, the relatively high rate of incorporation of radioactivity derived from [3H]glucose (localized in the phospholipid glycerol backbone) was in contrast with results of studies using brain slices and ["Ciglucose, in which phosphatidylcholine was the major contributor (Lapetina & Michell, 1974). This would emnphasize the relatively high rate of synthesis de novo of phosphatidylinositol at the nerve terminal as compared with whole brain and underline the possible functional importance of this phosphoglyceride at the synapse.…”
Section: Discussioncontrasting
confidence: 50%
See 1 more Smart Citation
“…However, on the basis of data for the release of unesterified fatty acids in rat brain post mortem (Bazan, 1970) and from incubated cerebral-cortex synaptosomal fractions (Price & Rowe, 1972), the higher rate of incorporation of oleate than of arachidonate into the D fraction phospholipid (Table 1) (Holub et al, 1970;Baker & Thompson, 1972;Breckenridge et al, 1973). However, the relatively high rate of incorporation of radioactivity derived from [3H]glucose (localized in the phospholipid glycerol backbone) was in contrast with results of studies using brain slices and ["Ciglucose, in which phosphatidylcholine was the major contributor (Lapetina & Michell, 1974). This would emnphasize the relatively high rate of synthesis de novo of phosphatidylinositol at the nerve terminal as compared with whole brain and underline the possible functional importance of this phosphoglyceride at the synapse.…”
Section: Discussioncontrasting
confidence: 50%
“…This was also found to be the case in some preliminary studies with [3H] (Miller & Dawson, 1972a) and in vitro (Yagihara et aL., 1973) (Lunt & Lapetina, 1970) with synaptosomal preparations indicated a similar order of labelling of these phosphoglycerides. However, work with [14C]-glucose and brain slices (Lapetina & Michell, 1974) (P1) were incubated in the presence of radioactive compounds for 15 min at 37°C. After the incubation the P2 fractions were washed, hypo-osmotically disrupted and a derived supernatant fraction (Ws) was fractionated by discontinuous-sucrose-gradient centrifugation.…”
Section: Radioactive Labelling Ofp2 Fractionsmentioning
confidence: 99%
“…The data of Wells and Wells (5) (18)(19)(20). By using methods which allow a more direct measurement of Ptdlns concentration, we were able to show a definite loss of the phospholipid in response to AcCho, strongly suggesting stimulation of the enzyme PtdIns-Ins phosphohydrolase by the neurotransmitter.…”
Section: Discussionmentioning
confidence: 82%
“…While InslP, the principal labeled metabolite, could theoretically be derived from the phosphodiesteratic breakdown of PtdIns, three considerations militate against this possibility. First, the mAChR-mediated breakdown of PtdIns has yet t o be demonstrated in brain (Abdel-Latif e t al., 1974;Lapetina and Michell, 1974;Fisher and Agranoff, 1981). Second, there is indirect evidence from nerve-ending preparations that the initial event is the breakdown of PtdIns4P and/or PtdIns(4,5)P2 rather than that of PtdIns (Fisher and Agranoff, 19811, and third, the early release of Ins(] ,4)P, and Ins( 1 ,4,5)P3 following carbamoylcholine stimulation of brain slices has recently been reported (Bemdge et al, 1983).…”
Section: Discussionmentioning
confidence: 99%