Effects of Acclimation, Population, and Sex on Behavioral Thermoregulation, CTMax, Symptoms of Heat Stress, and Gene Expression of Melanoplus differentialis, a Generalist Grasshopper—Does Temporal Thermal Heterogeneity Prepare Populations for a Warming World?

Abstract

Insects thermoregulate using both canalized and plastic mechanisms. Populations of insects utilize these mechanisms to different extents, and while it is posited that the degree of thermal uctuation a population experiences can determine the optimal combination of mechanisms to utilize, this is still being elucidated. We used three populations of the generalist grasshopper, Melanoplus differentialis (Thomas, 1856), from sites experiencing different degrees of thermal heterogeneity to test for correlations between thermal heterogeneity and 1) behavioral thermoregulation, 2) upper temperature tolerance, 3) the ability to thermally acclimate, and 4) gene expression. We found that 1) behavioral thermoregulation did not differ among sites, 2) CT Max of males, but not females, was higher at more thermally heterogeneous sites, 3) there was acclimation in some of the tested traits, but thermally heterogeneous sites did not always have the most plastic individuals, and 4) there were differences in gene expression among sites, but these differences were not between the most and least thermally heterogeneous sites. We concluded that thermal heterogeneity may play a selective role in some, but not all, of the measured thermoregulatory traits and their plasticity.

Body mass, temperature, and pathogen intensity differentially affect critical thermal maxima and their population‐level variation in a solitary bee

“…pruinosa may have performed better in our CT max trials due to increased water retention. To our knowledge, our finding that the strength of this relationship can be sex‐specific is not reported among bees; however, sex differences in thermal tolerance traits (Laidlaw et al., 2020 ; Madeira et al., 2012 ; Missionário et al., 2022 ; Mitchell & Hoffmann, 2010 ), plasticity (Pottier et al., 2021 ), or in the impacts of the environment on thermal tolerance (Preston & Johnson, 2022 ) have been reported across several other taxonomic groups. Generally, studies that thoroughly consider potential sex differences are rare or do not find differences (Du et al., 2000 ; Gonzalez, Oyen, Aguilar, et al., 2022 ; González‐del‐Pliego et al., 2020 ).…”