Effects of absorbent treatment on the purification of paclitaxel from cell cultures of Taxus chinensis and yew tree

Development and optimization of a novel simultaneous microwave-assisted extraction and adsorbent treatment process for separation and recovery of paclitaxel from plant cell cultures

“…These compounds must be removed from the pretreatment process because they have an adverse effect on the lifetime of the column's packing material (resin) in the final purification process using HPLC. The conventional method introduces an adsorbent treatment into paclitaxel separation/purification for removal of the unwanted compounds [3,7,16], making the process complex and expensive. Therefore, in this study, we developed the first simultaneous MAE and adsorbent treatment process to recover the anticancer agent paclitaxel from plant cell cultures and effectively remove plant-derived tar and waxy compounds at the same time, thus simplifying the separation/purification process.…”

Enhanced performance of a tandem simulated moving bed process for separation of paclitaxel, 13-dehydroxybaccatin III, and 10-deacetylpaclitaxel by making a difference between the adsorbent particle sizes of the two subordinate simulated moving bed units

“…The ternary paclitaxel-related mixture, which was available in the literature [4,8,9], was chosen as an example system for investigating the effectiveness of the proposed strategy for the tandem SMB performance. The length and diameter of each column in the tandem SMB under investigation were set at 15 cm and 3 cm, respectively, and the column configuration in each ring was fixed at 2-2-2-2.…”

“…In the previous section, the selectivities among the three components remained unchanged because the three components under consideration came from the literature [4,7,8]. To investigate the effect of the selectivity related to the ternary separation on the advantage of the advanced process over the classical process, a series of the tandem-SMB optimizations were performed by reducing average-selectivity, which was defined here as the average of the A-B selectivity (H B /H A ) and the B-C selectivity (H C /H B ).…”

“…To facilitate this task, a conventional tandem SMB based on the use of the same particle size in both rings will be designated as classical tandem SMB process while the proposed tandem SMB that allows the use of different particle sizes in Ring I and Ring II will be designated as advanced tandem SMB process. Both processes will be optimized for maximizing their productivities under the same conditions while employing the ternary paclitaxel-related mixture (paclitaxel and its precursors such as 13-dehydroxybaccatin III and 10-deacetylpaclitaxel) [4,8,9] as an example ternary-separation system. The results from this optimization will then be used to compare the performances of the classical and the advanced processes.…”

Ultrasound-Assisted Liquid-Liquid Extraction for Recovery of Paclitaxel from Plant Cell Cultures

“…식물세포배양 방법은 다른 방법에 비해 외부 인자(기후, 환경)에 의한 영향을 받지 않고 생물반응기 내에서 안정 적으로 제품생산이 가능하기 때문에 일정한 품질의 파클리탁셀을 대량 생산할 수 있는 장점이 있다 [5,7]. 식물세포배양에 의하여 생산 된 파클리탁셀은 대부분 바이오매스(식물세포)에 포함되어 있으며 [8], 파클리탁셀 생산은 여러 단계의 추출 및 정제 공정으로 이루어 져 있다 [9][10][11] …”