Effects of abscisic acid or benzyladenine on pigment contents, chlorophyll fluorescence, and chloroplast ultrastructure during water stress and after rehydration

Haisel, D.; Pospíšilová, Jana; Synková, Helena; Schnablová, Renáta; Baťková, P.

doi:10.1007/s11099-006-0079-5

Cited by 52 publications

(39 citation statements)

References 51 publications

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“…Thus both transgenics with elevated endogenous CKs coped with water stress more efficiently than WT plants (at least in case of the water loss lower than 20 %). This was in agreement with the protection of photosynthetic pigments by application of benzyladenine (BA) found in previous experiments (Haisel et al 2006). Application of BA reduced the decrease in Chl also in water-stressed wheat (Chernyad′ev and Monakhova 2003) or in Cassia (Singh et al 2001).…”

Section: Discussionsupporting

confidence: 90%

“…Non-stomatal effects of CKs have been also reported (for recent review see Nyitrai 2005) including alleviation of the negative effects of water stress on chlorophyll (Chl) and carotenoid (Car) contents, photochemical activities of photosystem (PS) 1 and 2, and content and activity of ribulose-1,5-bisphosphate carboxylase or phosphoenol-pyruvate carboxylase (Metwally et al 1997, Chernyad′ev and Monakhova 1998, Pandey et al 2000/4, Singh et al 2001. Similarly, BA pre-treatments ceased a degradation of Chl and Car induced by water stress, increased contents of xanthophyll cycle pigments and decreased degree of their de-epoxidation (Haisel et al 2006). …”

Section: Introductionmentioning

confidence: 99%

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The impact of trans-zeatin O-glucosyltransferase gene over-expression in tobacco on pigment content and gas exchange

Haisel¹,

Vaňoková²,

Synková³

et al. 2008

Biol. plant.

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The responses of tobacco plants over-expressing trans-zeatin O-glucosyltransferase gene under constitutive or senescence-inducible promoter (35S:ZOG1 and SAG12:ZOG1) and of wild type (WT) plants to water stress and subsequent rehydration were compared. In plants sufficiently supplied with water, both transgenics have higher net photosynthetic rate (P N ) in upper and middle leaves and higher stomatal conductance (g s ) in middle leaves than WT. Water use efficiency (WUE = P N /E) was higher in both transgenics than in WT. During prolonged water stress, both P N and E declined to a similar extent in both transgenics and WT plants. However, 7 d after rehydration P N in SAG:ZOG (upper and middle leaves) and 35S:ZOG (upper leaves) was higher than that in WT plants. Increased content of endogenous CKs in 35S:ZOG plants did not prevent their response to ABA application and the results obtained did not support concept of CK antagonism of ABA-induced stomatal closure. The chlorophyll (Chl) a+b content was mostly higher in both transgenics than in WT. During water stress and subsequent rehydration it remained unchanged in upper leaves, decreased slightly in middle leaves only of WT, while rapidly in lower leaves. Total degradation of Chl, carotenoids and xanthophyll cycle pigments (XCP) was found under severe water stress in lower leaves. Carotenoid and XCP contents in middle and upper leaves mostly increased during development of water stress and decreased after rehydration. While β-carotene content was mostly higher in WT, neoxanthin content was higher in transgenics especially in 35S:ZOG under severe stress and after rehydration. The higher content of XCP and degree of their deepoxidation were usually found in upper and middle leaves than in lower leaves with exception of SAG:ZOG plants during mild water stress.

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Section: Discussionsupporting

confidence: 90%

Section: Introductionmentioning

confidence: 99%

The impact of trans-zeatin O-glucosyltransferase gene over-expression in tobacco on pigment content and gas exchange

Haisel¹,

Vaňoková²,

Synková³

et al. 2008

Biol. plant.

Self Cite

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“…3). Similar reports on chloroplast structure have also been made earlier with the application of BA under water stress in bean and sugarbeat (Haisel et al, 2006). RWC reduced under water deficit stress (Fig.…”

Section: And Ennajeh Etsupporting

confidence: 88%

Bioregulators protected the leaf anatomy and photosynthetic machinery under water deficit stress in chickpea (Cicer arietinum L.)

Kumar

Singh

2016

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Water deficit is undoubtedly one of the most important environmental stresses limiting the productivity of chickpea around the world. Exogenous application of bioregulators has been found to be a novel technology for imparting stress tolerance in crop plants. This study evaluated the changes brought about by two bioregulators viz., thiourea (TU) and thidiazuron (TDZ) under water deficit stress in chickpea leaf anatomy, chloroplast ultrastructure and photosynthesis. The experiment was conducted using Pusa 362 (Desi type) chickpea variety. Imposed water deficit treatment decreased relative water content (RWC), membrane stability index (MSI) and photosynthetic rate (P N ). However, bioregulators application maintained higher RWC, MSI and P N under water deficit stress. Under imposed water stress, compact palisade layers of the mesophyll tissue were disrupted and cell size of the mesophyll cells displayed drastic reduction. Chloroplast, under water stress, displayed a number of grana with lose type of thylakoid, large increase in osmiophillic granules, reduction in the amount of starch granules and overall disruption of the thylakoid membrane. Foliar application of bioregulators maintained the integrity of mesophyll tissue and chloroplast structure thereby protected the chickpea plants from the detrimental effects of water deficit stress.

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“…Dias et al (2014) reported that foliar application of ABA promoted net CO 2 assimilation rate, plant dry mater accumulation and antioxidant enzyme activity. Several studies also demonstrated that ABA pre-treatment ameliorated negative effect of water stress in naturally grown barley, bean, maize, sugar beet and tobacco (Agarwal et al 2005;Haisel et al 2006;Mizrahi et al 1974). …”

Section: Resultsmentioning

confidence: 98%

Improvement ofex vitroacclimatization of mulberry plantlets by supplement of abscisic acid to the last subculture medium

2017

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Mulberry (Morus sp.) of the family Moraceae is very economically important in Asian countries including Korea, because its leaf and fruit have been commercially used in sericulture and horticultural industries. Therefore it is necessary to develop the optimal production system for rapid and cost-effective propagation of mulberry. Our studies focused on establishing an acclimatization method for the successful plantlet production of new cultivar 'Cheongsu' which was transferred ex vitro after in vitro culture. In particular, effect of abscisic acid (ABA) addition into the last subculture medium on plantlet response to subsequent ex vitro transfer and its growth was investigated. During acclimatization, stomatal conductance and transpiration rate of ABA-pretreated plantlets were significantly lower than those of non-treated plantlets. Net photosynthetic rate of ABA-pretreated plantlets decreased after ex vitro transfer but increased after 14 days, and it was mostly higher than that of non-treated plantlets. Moreover, relative water content as well as chlorophyll contents and its ratio were also higher in ABA-pretreated plantlets. On the other hand, proline was considerably higher than in control plantlets. After 1 month of ex vitro transfer, survival rate of ABA-pretreated plantlets was 85.6%, which increased by 29.1% in comparison with control (56.5%). More vigorous growth was also observed in ABA-pretreated plantlets. From these results, it was found that application of ABA to the last subculture medium could improve acclimatization and promote survival of mulberry plantlets after ex vitro transfer, inducing water stress tolerance and alleviating abiotic stresses.Keywords Mulberry, Abscisic acid, Ex vitro transfer, Acclimatization, Water stress Introduction Mulberry (Morus sp.) is an economically important tree grown in India, China, Korea and several Asian countries where its foliage is used as food for silkworms in sericulture industry (Vijayan et al. 2012). It is also commercially valuable in the horticulture, food and cosmetic industries, in particular, its phytochemical and medicinal properties such as antioxidants (Yen et al. 1996) and hypoglycemia compounds (Kelkar et al. 1996) have been widely used as healthcare products. These days it is cultivated for fruit production, which is used for human consumption including jam, jelly, frozen desserts, pulp, juice and wine (Koyuncu 2004). Mulberry fruit is regarded as a traditional medicine for dysentery, constipation and avulsed teeth due to rich phenolic acids and flavonoids (Arfan et al. 2012; Lee and Bae 2011).Plant micropropagation has been widely applied to agriculture industry for large scale production of economically important and valuable species. But its commercial use is limited in many species due to the low plantlet survival rates during the acclimatization (Pospisilova et al. 2009a, b), which are known to be related to an abnormal morphology, anatomy and physiology under in vitro culture conditions (Dias et al. 2013a). Poor functioning of...

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Effects of abscisic acid or benzyladenine on pigment contents, chlorophyll fluorescence, and chloroplast ultrastructure during water stress and after rehydration

Cited by 52 publications

References 51 publications

The impact of trans-zeatin O-glucosyltransferase gene over-expression in tobacco on pigment content and gas exchange

The impact of trans-zeatin O-glucosyltransferase gene over-expression in tobacco on pigment content and gas exchange

Bioregulators protected the leaf anatomy and photosynthetic machinery under water deficit stress in chickpea (Cicer arietinum L.)

Improvement ofex vitroacclimatization of mulberry plantlets by supplement of abscisic acid to the last subculture medium

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