Effects of ABA on primary terpenoids and Δ9-tetrahydrocannabinol in Cannabis sativa L. at flowering stage

Mansouri, Hakimeh; Asrar, Zahra; Szopa, Jan

doi:10.1007/s10725-009-9375-y

Cited by 56 publications

(53 citation statements)

References 42 publications

(43 reference statements)

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“…Positive effects of ABA in increasing of chlorophyll content have been reported by Agarwal et al (2005). At previous work we found that at flowering stage ABA decreased the chlorophyll content in both male and female Cannabis plants (Mansouri et al 2009). This result indicates that plant response to ABA is different at various stages of development.…”

Section: ⎯⎯⎯⎯supporting

confidence: 57%

“…Chlorophyll and carotenoids in leaves were determined as described by Lichtenthaler (1987), and α-tocopherol was extracted according to Panfili et al (2003) and analyzed as described previously (Mansouri et al 2009). Quantitative analysis of squalene and phytosterols (campesterol, β-sitosterol and stigmasterol) followed the procedure of Hartmann and Benveniste (1987).…”

Section: ⎯⎯⎯⎯mentioning

confidence: 99%

“…Quantitative analysis of squalene and phytosterols (campesterol, β-sitosterol and stigmasterol) followed the procedure of Hartmann and Benveniste (1987). Cannabinoids were extracted and analyzed as described previously (Mansouri et al 2009). …”

Section: ⎯⎯⎯⎯mentioning

confidence: 99%

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Effects of abscisic acid on content and biosynthesis of terpenoids in Cannabis sativa at vegetative stage

Mansouri

Asrar

2012

Biologia plant.

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The influence of abscisic acid (ABA) on plastidial and cytosolic terpenoids and on two key enzymes for terpenoid biosynthesis was determined in vegetative stage of Cannabis sativa L. Low concentration of ABA (1 µM) increased 1-deoxy-D-xylulose 5-phosphate synthase (DXS) activity in treated plants in comparison to control plants. The amounts of chlorophyll a and carotenoids increased in response to ABA treatment but chlorophyll b content declined. The accumulation of α-tocopherol was stimulated only by 10 µM ABA. The ABA-treated plants showed a decline in 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGR) activity which was followed by a decrease in squalene and phytosterol content. ABA also decreased tetrahydrocannabinol (THC) and cannabidiol (CBD) contents. The essential oil had higher ratios of monoterpenes to sesquiterpenes as ABA-treated plants had less numbers of sesquiterpenes in comparison with control plants. Influence of ABA on the amounts of sesquiterpenes was different, some of them showed decrease of content and others increase of content.

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Section: ⎯⎯⎯⎯supporting

confidence: 57%

Section: ⎯⎯⎯⎯mentioning

confidence: 99%

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Effects of abscisic acid on content and biosynthesis of terpenoids in Cannabis sativa at vegetative stage

Mansouri

Asrar

2012

Biologia plant.

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“…ABA (synthesized via the MEP pathway) has been found to induce the biosynthesis of MEP-derived metabolites, and both ABA-dependent and -independent signaling pathways appear to be involved in osmotic stress tolerance (Huang et al, 2012). GA 3 has been reported to inhibit the biosynthesis of MEP-derived isoprenoids and to stimulate the MVA pathway (Mansouri et al, 2009). Additionally, ROS also could play the role of such regulators, since their signaling network controls diverse stress responses (Lin and Aarts, 2012).…”

Section: Discussionmentioning

confidence: 99%

Modeling of Dolichol Mass Spectra Isotopic Envelopes as a Tool to Monitor Isoprenoid Biosynthesis

et al. 2017

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The cooperation of the mevalonate (MVA) and methylerythritol phosphate (MEP) pathways, operating in parallel in plants to generate isoprenoid precursors, has been studied extensively. Elucidation of the isoprenoid metabolic pathways is indispensable for the rational design of plant and microbial systems for the production of industrially valuable terpenoids. Here, we describe a new method, based on numerical modeling of mass spectra of metabolically labeled dolichols (Dols), designed to quantitatively follow the cooperation of MVA and MEP reprogrammed upon osmotic stress (sorbitol treatment) in Arabidopsis (Arabidopsis thaliana). The contribution of the MEP pathway increased significantly (reaching 100%) exclusively for the dominating Dols, while for long-chain Dols, the relative input of the MEP and MVA pathways remained unchanged, suggesting divergent sites of synthesis for dominating and long-chain Dols. The analysis of numerically modeled Dol mass spectra is a novel method to follow modulation of the concomitant activity of isoprenoid-generating pathways in plant cells; additionally, it suggests an exchange of isoprenoid intermediates between plastids and peroxisomes.Isopentenyl diphosphate (IPP), the building block of isoprenoids, the most numerous class of secondary metabolites, is derived in plants from two pathways operating in parallel: the cytoplasmic mevalonate (MVA) and the plastidic methylerythritol phosphate (MEP) pathways (Rohmer, 1999;Pulido et al., 2012).Analysis of over 130 isoprenoids has shown that, in angiosperms under standard growth conditions, carotenoids and chlorophyll phytyl chains on the one hand and phytosterols on the other are made nearly exclusively via a single pathway (MEP and MVA, respectively), while numerous other isoprenoids, including dolichols (Dols; Skorupinska-Tudek et al., 2008), are of mixed origin (Hemmerlin et al., 2012).The cooperation of the two pathways (often called cross talk) is considered essential for plant adaptive responses to biotic and abiotic stresses. Consequently, their relative contributions to the formation of IPP are modulated, and the expression of particular genes of the MVA and MEP pathways is frequently correlated with stress (pathogen attack, elicitation, wounding, etc.;Hemmerlin et al., 2012) or plant development (Opitz et al., 2014). Interestingly, the effect of stress on the regulation of the MEP-MVA balance has so far been analyzed only qualitatively.Several methodologies have been employed to unravel the relative contributions of the MVA and MEP pathways to isoprenoids, such as metabolic labeling with isotopically labeled precursors (stable isotopes or radioisotopes were used), followed by structural analysis of the product of interest or blockage of the pathway (chemical, with pathway-specific inhibitors, or genetic), followed by quantification of the isoprenoid intermediates and/or end products. The advantages

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“…ABA can activate the SnRK2 protein, which phosphorylates downstream transcription factors and allows the corresponding genes to be expressed (Shan et al 2012). Moreover, Mansouri et al (2009) have discovered that ABA enhances the activity of 1-deoxy-D-xylulose 5-phosphate synthase, which is another essential enzyme in the MEP pathway. Therefore, ABA and GA simultaneously increase the expression of HMGR and DXR as well as their corresponding enzyme activities, which subsequently promote essential oil synthesis in A. lancea.…”

Section: Discussionmentioning

confidence: 99%

Reactive oxygen species and hormone signaling cascades in endophytic bacterium induced essential oil accumulation in Atractylodes lancea

Zhou

Zhao

et al. 2016

Planta

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Pseudomonas fluorescens induces gibberellin and ethylene signaling via hydrogen peroxide in planta . Ethylene activates abscisic acid signaling. Hormones increase sesquiterpenoid biosynthesis gene expression and enzyme activity, inducing essential oil accumulation. Atractylodes lancea is a famous Chinese medicinal plant, whose main active components are essential oils. Wild A. lancea has become endangered due to habitat destruction and over-exploitation. Although cultivation can ensure production of the medicinal material, the essential oil content in cultivated A. lancea is significantly lower than that in the wild herb. The application of microbes as elicitors has become an effective strategy to increase essential oil accumulation in cultivated A. lancea. Our previous study identified an endophytic bacterium, Pseudomonas fluorescens ALEB7B, which can increase essential oil accumulation in A. lancea more efficiently than other endophytes; however, the underlying mechanisms remain unknown (Physiol Plantarum 153:30-42, 2015; Appl Environ Microb 82:1577-1585, 2016). This study demonstrates that P. fluorescens ALEB7B firstly induces hydrogen peroxide (H2O2) signaling in A. lancea, which then simultaneously activates gibberellin (GA) and ethylene (ET) signaling. Subsequently, ET activates abscisic acid (ABA) signaling. GA and ABA signaling increase expression of HMGR and DXR, which encode key enzymes involved in sesquiterpenoid biosynthesis, leading to increased levels of the corresponding enzymes and then an accumulation of essential oils. Specific reactive oxygen species and hormone signaling cascades induced by P. fluorescens ALEB7B may contribute to high-efficiency essential oil accumulation in A. lancea. Illustrating the regulation mechanisms underlying P. fluorescens ALEB7B-induced essential oil accumulation not only provides the theoretical basis for the inducible synthesis of terpenoids in many medicinal plants, but also further reveals the complex and diverse interactions among different plants and their endophytes.

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Effects of ABA on primary terpenoids and Δ9-tetrahydrocannabinol in Cannabis sativa L. at flowering stage

Cited by 56 publications

References 42 publications

Effects of abscisic acid on content and biosynthesis of terpenoids in Cannabis sativa at vegetative stage

Effects of abscisic acid on content and biosynthesis of terpenoids in Cannabis sativa at vegetative stage

Modeling of Dolichol Mass Spectra Isotopic Envelopes as a Tool to Monitor Isoprenoid Biosynthesis

Reactive oxygen species and hormone signaling cascades in endophytic bacterium induced essential oil accumulation in Atractylodes lancea

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