Effects of a single bout of isometric neuromuscular electrical stimulation on rat gastrocnemius muscle: A combined functional, biochemical and MRI investigation

Gondin, Julien; Giannesini, Benoı̂t; Vilmen, Christophe; Fur, Yann Le; Cozzone, Patrick J.; Bendahan, David

doi:10.1016/j.jelekin.2011.01.006

Cited by 3 publications

(4 citation statements)

References 46 publications

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“…Biochemical monitoring for muscle injury showed no adverse effects in an extended follow-up. This is in agreement with recent results that confirm the safety of NMES application [51,52]. …”

Section: Discussionsupporting

confidence: 94%

Acute microcirculatory effects of medium frequency versus high frequency neuromuscular electrical stimulation in critically ill patients - a pilot study

Angelopoulos

Karatzanos

Dimopoulos

et al. 2013

Ann. Intensive Care

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BackgroundIntensive care unit-acquired weakness (ICUAW) is a common complication, associated with significant morbidity. Neuromuscular electrical stimulation (NMES) has shown promise for prevention. NMES acutely affects skeletal muscle microcirculation; such effects could mediate the favorable outcomes. However, optimal current characteristics have not been defined. This study aimed to compare the effects on muscle microcirculation of a single NMES session using medium and high frequency currents.MethodsICU patients with systemic inflammatory response syndrome (SIRS) or sepsis of three to five days duration and patients with ICUAW were studied. A single 30-minute NMES session was applied to the lower limbs bilaterally using current of increasing intensity. Patients were randomly assigned to either the HF (75 Hz, pulse 400 μs, cycle 5 seconds on - 21 seconds off) or the MF (45 Hz, pulse 400 μs, cycle 5 seconds on - 12 seconds off) protocol. Peripheral microcirculation was monitored at the thenar eminence using near-infrared spectroscopy (NIRS) to obtain tissue O2 saturation (StO2); a vascular occlusion test was applied before and after the session. Local microcirculation of the vastus lateralis was also monitored using NIRS.ResultsThirty-one patients were randomized. In the HF protocol (17 patients), peripheral microcirculatory parameters were: thenar O2 consumption rate (%/minute) from 8.6 ± 2.2 to 9.9 ± 5.1 (P = 0.08), endothelial reactivity (%/second) from 2.7 ± 1.4 to 3.2 ± 1.9 (P = 0.04), vascular reserve (seconds) from 160 ± 55 to 145 ± 49 (P = 0.03). In the MF protocol: thenar O2 consumption rate (%/minute) from 8.8 ± 3.8 to 9.9 ± 3.6 (P = 0.07), endothelial reactivity (%/second) from 2.5 ± 1.4 to 3.1 ± 1.7 (P = 0.03), vascular reserve (seconds) from 163 ± 37 to 144 ± 33 (P = 0.001). Both protocols showed a similar effect. In the vastus lateralis, average muscle O2 consumption rate was 61 ± 9%/minute during the HF protocol versus 69 ± 23%/minute during the MF protocol (P = 0.5). The minimum amplitude in StO2 was 5 ± 4 units with the HF protocol versus 7 ± 4 units with the MF protocol (P = 0.3). Post-exercise, StO2 increased by 6 ± 7 units with the HF protocol versus 5 ± 4 units with the MF protocol (P = 0.6). These changes correlated well with contraction strength.ConclusionsA single NMES session affected local and systemic skeletal muscle microcirculation. Medium and high frequency currents were equally effective.

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“…Biochemical monitoring for muscle injury showed no adverse effects in an extended follow-up. This is in agreement with recent results that confirm the safety of NMES application [51,52]. …”

Section: Discussionsupporting

confidence: 94%

Acute microcirculatory effects of medium frequency versus high frequency neuromuscular electrical stimulation in critically ill patients - a pilot study

Angelopoulos

Karatzanos

Dimopoulos

et al. 2013

Ann. Intensive Care

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“…NMES mice were then submitted to a NMES protocol performed under isometric conditions at a submaximal mechanical intensity corresponding to ∼15% of F max in order to i) avoid muscle damage (Gondin et al, 2011a); ii) mimic the application of NMES in severely impaired patients (Maddocks et al, 2016) for whom higher force levels are difficult to reach due to discomfort associated with electrical stimuli (Gondin et al, 2011c). As a consequence, the current intensity was carefully adjusted at the beginning of each NMES training session in order to reach 15% of F max (i.e., initial intensity I 15% ; range: 12.5-17.5% of F max ) in response to a 250-ms 50 Hz stimulation train (Fig.…”

Section: Experimental Devicementioning

confidence: 99%

“…Here, we developed a new and individualized resistance training protocol which relies on the main function of skeletal muscle, i.e., its ability to produce force in response to repeated electrical stimuli. Thanks to our large experience in neuromuscular electrical stimulation (NMES) training (Gondin et al, 2005(Gondin et al, , 2006(Gondin et al, , 2011b, we designed a short NMES training protocol that was performed under isometric conditions to avoid muscle damage (Gondin et al, 2011a). In addition, the force produced by the plantar flexor muscles was adjusted to reach a submaximal level (i.e., around 15% of maximal force) and was carefully monitored in response to each stimulation train and for each trained mouse.…”

Section: Introductionmentioning

confidence: 99%

Individualized isometric neuromuscular electrical stimulation training promotes myonuclear accretion in mouse skeletal muscle

Zavoriti

Fessard

Rahmati

et al. 2021

Preprint

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Skeletal muscle is a plastic tissue that adapts to exercise through fusion of muscle stem cells (MuSCs) with myofibers, a physiological process referred to as myonuclear accretion. However, it is still unclear whether myonuclear accretion is driven by increased mechanical loading per se, or occurs, at least in part, in response to exercise-induced muscle injury. Here, we developed a carefully monitored and individualized neuromuscular electrical stimulation (NMES) training protocol of the mouse plantar flexor muscles. Each NMES training session consisted of 80 isometric contractions at a submaximal mechanical intensity corresponding to ~ 15% of maximal tetanic force to avoid muscle damage. NMES trained mice were stimulated for 2 × 3 consecutive days separated by one day of rest, for a total of 6 sessions. Experiments were conducted on C57BL/6J and BALB/c males at 10-12 weeks of age. NMES led to a robust myonuclear accretion and higher MuSC content in gastrocnemius muscle of both mouse lines, without overt signs of muscle damage/regeneration or muscle hypertrophy or force improvement. This new mouse model of myonuclear accretion relying on the main function of skeletal muscles, i.e., force production in response to electrical stimuli, will be of utmost interest to further understand the role of MuSCs in skeletal muscle adaptations.

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“…Damage to cell membranes in the muscles causes a CK efflux from the cells, leading to an increase of the CK concentration in the blood. Therefore, CK has been used as a marker of muscle damage [27][28][29]. In addition, aldolase is present in tissues that require high levels of energy metabolism, such as the skeletal muscles.…”

Section: Biochemical Blood Testsmentioning

confidence: 99%

Considerations for safety of high-frequency repetitive peripheral magnetic stimulation of skeletal muscles in rats: Assessment by histological analysis of muscles and biochemical blood tests

Suzuki

Hiraoka

Tsubahara

et al. 2015

Jpn J Compr Rehabil Sci

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Objective:The purpose of the present study was to investigate the safety of high-frequency repetitive peripheral magnetic stimulation (rpMS) of skeletal muscles using histological analyses of muscles and biochemical blood tests. Methods: Twenty male Wistar rats were divided into the rpMS group that received rpMS and the control group (CON group) that did not receive rpMS. Hematoxylin and eosin, modified Gomori trichrome, NADH-tetrazolium reductase, acid phosphatase, and Periodic acid-Schiff (PAS) stains were used for histological analyses of the muscles. Creatine kinase, aldolase, and lactate dehydrogenase (LDH) were measured for the biochemical blood tests. Additionally, the histological analyses of the muscles were performed in two rats at 4 and 12 hours after completion of rpMS application. Results: PAS stain showed glycogen depletion in the muscle fibers of the rpMS group. However, this finding was not observed in the rats at 4 hours after rpMS application. There was a significantly larger LDH decrease in the rpMS group compared with the CON group. No significant differences were observed for any of the other staining methods or serum enzymes between the two groups. There were no other abnormal findings in the rpMS group. Conclusion: It was established that rpMS could be utilized safely on skeletal muscles since it did not cause muscle damage in this study.

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Effects of a single bout of isometric neuromuscular electrical stimulation on rat gastrocnemius muscle: A combined functional, biochemical and MRI investigation

Cited by 3 publications

References 46 publications

Acute microcirculatory effects of medium frequency versus high frequency neuromuscular electrical stimulation in critically ill patients - a pilot study

Acute microcirculatory effects of medium frequency versus high frequency neuromuscular electrical stimulation in critically ill patients - a pilot study

Individualized isometric neuromuscular electrical stimulation training promotes myonuclear accretion in mouse skeletal muscle

Considerations for safety of high-frequency repetitive peripheral magnetic stimulation of skeletal muscles in rats: Assessment by histological analysis of muscles and biochemical blood tests

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