Effects of a resistance‐training programme on endoplasmic reticulum unfolded protein response and mitochondrial functions in PBMCs from elderly subjects

Abstract

Aging has been related with a decline in the ability to handle protein folding, which leads to endoplasmic reticulum stress and alterations in unfolded protein response (UPR). Importantly, physical activity could activate the UPR and attenuate or prevent age-induced ER dysfunction. The current study evaluated the effects of a resistance exercise on UPR and mitochondrial functions in peripheral blood mononuclear cells (PBMCs) from elderly subjects. Thirty healthy women and men (age, 72.8, s x− = 2.2 years) were randomized to a training group, which performed an 8-week resistance training programme, or a control group, which followed their daily routines. The phosphorylation of PERK and IRE1a, as well as ATF4, and XBP1 protein expression, significantly increased following the training, while expression of BiP, AFT6 and CHOP remain without changes. Additionally, the intervention also induced an increase in PGC-1a and Mfn1 protein levels, while no changes were found in Drp1 expression. Finally, the resistance protocol was not able to activate PINK1/ Parkin and Bnip3/Nix pathways. The results obtained seem to indicate that 8-week resistance exercise activates the UPR, stimulates mitochondrial biogenesis, maintains mitochondrial dynamics and prevents mitophagy activation by unfolded proteins in PBMCs from elderly subjects AQ2 ¶ .

Physical Exercise and Selective Autophagy: Benefit and Risk on Cardiovascular Health

“…Exhaustive exercise (EE) showed reduced LC3-II/LC3-I ratio, while exercise preconditioning (EP) transiently activated autophagy (especially at 2 h after EP) and attenuated EE-induced myocardial injury, which indicated preserved basal autophagy might underlie EP-offered benefit [112]. Besides endurance exercise, a study conducted by Estebanez and colleagues depicted that 8-week resistance exercise training prevented activation of mitophagy in peripheral blood mononuclear cells from otherwise healthy elderly individuals [113].…”

Efecto de un programa de ejercicio físico sobre la respuesta a proteínas mal plegadas en las células mononucleares de la sangre periférica de pacientes pediátricos con obesidad

“…Por último, los niveles de ATF6 p50 y los de su diana transcripcional BiP no se vieron afectados por el entrenamiento. En este mismo sentido, en un estudio con PBMC de ancianos, la expresión de ATF6 permaneció inalterada pese al entrenamiento (Estébanez et al, 2019).…”

Endoplasmic Reticulum-Mitochondria Contacts: A Potential Therapy Target for Cardiovascular Remodeling-Associated Diseases

“…In peripheral blood mononuclear cells (PBMCs) from healthy elderly subjects, 8-week resistance-training (RT) exercise-induced UPR activation counteracted ERS and led to mitochondrial improvement and prevention of mitophagy activation through increased ATF4, XBP1, PGC-1α, and Mfn1 protein levels ( Estebanez et al, 2019 ). The results showed that RT resulted in the UPR, as a protective mechanism, and mitochondrial biogenesis to adapt to the exercise demands.…”