Effects of a new biostimulant on gene expression and metabolic responses of tomato plants

Contartese, Valeria; Garabello, Christian; Occhipinti, Andrea; Bertea, Cinzia M.

doi:10.17660/actahortic.2016.1148.4

Cited by 15 publications

(17 citation statements)

References 15 publications

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“…For example, the treatment with lipophilic components of the brown seaweed Ascophyllum nodosum improved freezing tolerance in Arabidopsis thaliana by inducing the upregulation of 463 genes and the downregulation of 650 genes [ 40 ]. Moreover, Contartese et al (2016) similarly observed a higher number of downregulated genes (2415) as compared with the upregulated genes (1731) in tomato plants treated with Expando ® , a biostimulant developed to increase fruit size [ 41 ].…”

Section: Discussionmentioning

confidence: 99%

Transcriptome Analyses and Antioxidant Activity Profiling Reveal the Role of a Lignin-Derived Biostimulant Seed Treatment in Enhancing Heat Stress Tolerance in Soybean

Campobenedetto

Mannino

Agliassa

et al. 2020

Plants

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Soybean (Glycine max Merr.) is a worldwide important legume crop, whose growth and yield are negatively affected by heat stress at germination time. Here, we tested the role of a biostimulant based on lignin derivatives, plant-derived amino acids, and molybdenum in enhancing soybean heat stress tolerance when applied on seeds. After treatment with the biostimulant at 35 °C, the seed biometric parameters were positively influenced after 24 h, meanwhile, germination percentage was increased after 72 h (+10%). RNA-Seq analyses revealed a modulation of 879 genes (51 upregulated and 828 downregulated) in biostimulant-treated seeds as compared with the control, at 24 h after incubation at 35 °C. Surprisingly, more than 33% of upregulated genes encoded for ribosomal RNA (rRNA) methyltransferases and proteins involved in the ribosome assembly, acting in a specific protein network. Conversely, the downregulated genes were involved in stress response, hormone signaling, and primary metabolism. Finally, from a biochemical point of view, the dramatic H2O2 reduction 40%) correlated to a strong increase in non-protein thiols (+150%), suggested a lower oxidative stress level in biostimulant-treated seeds, at 24 h after incubation at 35 °C. Our results provide insights on the biostimulant mechanism of action and on its application for seed treatments to improve heat stress tolerance during germination.

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Section: Discussionmentioning

confidence: 99%

Transcriptome Analyses and Antioxidant Activity Profiling Reveal the Role of a Lignin-Derived Biostimulant Seed Treatment in Enhancing Heat Stress Tolerance in Soybean

Campobenedetto

Mannino

Agliassa

et al. 2020

Plants

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“…Treating sugar beet leaves with 1:1000 (0.5 mg C L −1 ) BLACKJAK resulted in higher sugar content (1.08%) and yield (6.78%) as compared with untreated plants. It has been demonstrated that biostimulants enhance processes such as photosynthesis, senescence, modulation of phytohormones, uptake of nutrients, resulting in higher performance and yield in plants [20]. In a study with sugar beet, the application of BLACKJAK increased sugar content (17.38%) and root yield than control demonstrating the positive effect of this biostimulant on sugar and plant growth [33].…”

Section: Discussionmentioning

confidence: 99%

“…Bv_AHG3 and Bv_ATTIR1, up-regulated after 48 and 72 h time exposure with BLACKJAK, are abscisic acid and auxin-related genes, respectively. Biostimulants that are developed from humic substances affect plant hormonal status [20]. It is possible that de novo synthesis of hormones might be induced in response to treating plants with humic based biostimulants.…”

Section: Discussionmentioning

confidence: 99%

“…It is possible that de novo synthesis of hormones might be induced in response to treating plants with humic based biostimulants. Amino acids, glycosides, polysaccharides, and organic acids are contained in many biostimulants and they may act as precursors or activators of endogenous plant hormones [20]. Abscisic acid (ABA) is involved in embryogenesis, seed physiology reactions, plant growth, and biotic and abiotic stress tolerance [36].…”

Section: Discussionmentioning

confidence: 99%

“…Specific functions and mechanisms of actions have not yet been determined for many biostimulant products [20]. The acquisition of a detailed knowledge of the molecular mechanisms underlying biostimulants helps to understand the biological functions of such substances.…”

Section: Introductionmentioning

confidence: 99%

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Expression Profiling of Candidate Genes in Sugar Beet Leaves Treated with Leonardite-Based Biostimulant

et al. 2019

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Leonardite-based biostimulants are a large class of compounds, including humic acid substances. Foliar application of biostimulants at field level improves plant growth, yield and quality through metabolic changes and stimulation of plant proton pumps. The present study aimed at identifying optimum dosage of BLACKJAK, a humic acid-based substance, which is able to modify genes involved in sugar beet growth. Thirty-three genes belonging to various biochemical pathway categories were tested in leaves of treated sugar beet (Beta vulgaris L.) samples to assess gene expression profiling in response to BLACKJAK. Seedlings of a diploid and multigerm variety were grown in plastic pots and sprayed with two dilutions of BLACKJAK (dilution 1:500–1.0 mg C L−1 and dilution 1:1000–0.5 mg C L−1). Leaf samples were collected after 24, 48, and 72 h treatment with BLACKJAK for each dilution. RNA was extracted and the quantification of gene expression was performed while using an OpenArray platform. Results of analysis of variance demonstrated that, 15 genes out of a total of 33 genes tested with OpenArray qPCR were significantly affected by treatment and exposure time. Analysis for annotation of gene products and pathways revealed that genes belonging to the mitochondrial respiratory pathways, nitrogen and hormone metabolisms, and nutrient uptake were up-regulated in the BLACKJAK treated samples. Among the up-regulated genes, Bv_PHT2;1 and Bv_GLN1 expression exerted a 2-fold change in 1:1000 and 1:500 BLACKJAK concentrations. Overall, the gene expression data in the BLACKJAK treated leaves demonstrated the induction of plant growth–related genes that were contributed almost to amino acid and nitrogen metabolism, plant defense system, and plant growth.

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Exploring Natural Resources for Biostimulants

Povero¹

2020

The Chemical Biology of Plant Biostimulants

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Effects of a new biostimulant on gene expression and metabolic responses of tomato plants

Cited by 15 publications

References 15 publications

Transcriptome Analyses and Antioxidant Activity Profiling Reveal the Role of a Lignin-Derived Biostimulant Seed Treatment in Enhancing Heat Stress Tolerance in Soybean

Transcriptome Analyses and Antioxidant Activity Profiling Reveal the Role of a Lignin-Derived Biostimulant Seed Treatment in Enhancing Heat Stress Tolerance in Soybean

Expression Profiling of Candidate Genes in Sugar Beet Leaves Treated with Leonardite-Based Biostimulant

Exploring Natural Resources for Biostimulants

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