Effects of a negative quaternary ion-sodium tetraphenylboron (TPB) on the rat phrenic nerve-diaphragm preparation

Guideri, Giancarlo; Seifter, Eli; Seifter, Joseph

doi:10.1016/0014-2999(72)90166-5

Cited by 8 publications

(2 citation statements)

References 11 publications

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“…For example hemicholinium with repetitive nerve stimulation produces a reduction of quantal size (Elmqvist & Quastel, 1965;Jones & Kwanbunbumpen, 1970). However, TPB does not inhibit choline acetyltransferase in vitro (Guideri, Seifter & Seifter, 1972) and the lack of antagonistic action of choline suggests that a competitive inhibition of choline uptake is unlikely. An alternative mechanism of action of TPB is an inhibition of vesicular storage of acetylcholine.…”

Section: Discussionmentioning

confidence: 99%

The Effects of Tetraphenylboron on Spontaneous Transmitter Release at the Frog Neuromuscular Junction

Marshall¹,

Parsons²

1975

British J Pharmacology

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1The effects of tetraphenylboron (TPB) on spontaneous transmitter release were studied in the frog sartonius muscle preparation. 2 TPB (0.001-1 mM) produced a time-dependent increase in miniature endplate potential (m.e.p.p.) activity that was not sustained. TPB (0.1 mM) produced similar effects on m.e.p.p. frequency in normal Ringer solutions, in the absence of Ca2+ or CF-and in the presence of excess Ca2+ and of tetrodotoxin. The effect of TPB (0.01 mM) was reduced but not abolished in the absence of Ca2+. probably by a combination of rapid release and concomitant inhibition of transmitter storage.

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Section: Discussionmentioning

confidence: 99%

The Effects of Tetraphenylboron on Spontaneous Transmitter Release at the Frog Neuromuscular Junction

Marshall¹,

Parsons²

1975

British J Pharmacology

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“…Among the substances which affect ACHE activity of whole erythrocytes is tetraphenylboron (TPB), a negative quaternary compound [6]. This substance does not inhibit the reac tion catalyzed by ACHE [6,7], but causes irreversibly enzyme inactivation when red cells are exposed to non-lytic concentrations (<600 [i.mol/1) of the reagent. Since other agents, such as l,5-difluoro-2,4-dinitrobenzene (DFDNB) and glutaraldehyde, not only inactivate red cell ACHE but also cause significant changes in the stability of the residual activity [8,9], it was of importance to ascertain the effect of TPB on this property of the enzyme.…”

Section: Introductionmentioning

confidence: 99%

Stability of Membrane Acetylcholinesterase in Human Erythrocytes Treated with Tetraphenylboron

Herz¹

1975

Enzyme

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Treatment of human erythrocytes with non-hemolytic concentrations of tetraphenylboron causes irreversible inactivation of acetylcholinesterase, an enzyme located at or near the outer cell surface. By contrast to other agents which also inactivate acetylcholinesterase activity such as 1,5-difluoro-2,4-dinitrobenzene and glutaraldehyde, tetraphenylboron does not affect the thermostability of the enzyme, alter its susceptibility to dénaturation by urea or hinder the proteolytic digestion of the erythrocyte membrane.

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Bor und spezielle Borverbindungen

Kliegel¹

1980

BOR in Biologie, Medizin Und Pharmazie

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Effects of a negative quaternary ion-sodium tetraphenylboron (TPB) on the rat phrenic nerve-diaphragm preparation

Cited by 8 publications

References 11 publications

The Effects of Tetraphenylboron on Spontaneous Transmitter Release at the Frog Neuromuscular Junction

The Effects of Tetraphenylboron on Spontaneous Transmitter Release at the Frog Neuromuscular Junction

Stability of Membrane Acetylcholinesterase in Human Erythrocytes Treated with Tetraphenylboron

Bor und spezielle Borverbindungen

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