Effects of a muramidase on a mixed bacterial community

Mercier, Carine; Domakova, Elena; Tremblay, Josselyne; Kulakauskas, Saulius

doi:10.1111/j.1574-6968.2000.tb09135.x

Cited by 22 publications

(15 citation statements)

References 20 publications

(21 reference statements)

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“…However, alterations in PG remodeling could also affect another C. jejuni factor required for biofilm development. Mutants in PG hydrolases or PG biosynthetic enzymes have also been shown to exhibit biofilm defects in several other organisms, such as E. coli (50, 51), Burkholderia cepacia (52), Streptococcus gordonii (53), and L. lactis (54); however, it also remains to be shown in these organisms what specific cell surface organelle or adhesin is affected. The Δ pgp2 biofilm defect along with the hyporeactivity this mutant displayed on CFW further supports the link between CFW reactivity and key C. jejuni pathogenic properties, as described previously (11, 14, 26).…”

Section: Discussionmentioning

confidence: 99%

Peptidoglycan ld-Carboxypeptidase Pgp2 Influences Campylobacter jejuni Helical Cell Shape and Pathogenic Properties and Provides the Substrate for the dl-Carboxypeptidase Pgp1

Frirdich

Vermeulen

Biboy

et al. 2014

Journal of Biological Chemistry

186

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Background: C. jejuni helical shape is important to pathogenesis.Results: Deletion of pgp2 results in loss of C. jejuni helical shape and change in peptidoglycan structure and pathogenic properties.Conclusion: Pgp2 is a ld-carboxypeptidase cleaving peptidoglycan tetrapeptides to tripeptides.Significance: Characterization of enzymes involved in C. jejuni peptidoglycan and cell shape maintenance is crucial to the understanding of fundamental properties of this organism.

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Section: Discussionmentioning

confidence: 99%

Peptidoglycan ld-Carboxypeptidase Pgp2 Influences Campylobacter jejuni Helical Cell Shape and Pathogenic Properties and Provides the Substrate for the dl-Carboxypeptidase Pgp1

Frirdich

Vermeulen

Biboy

et al. 2014

Journal of Biological Chemistry

186

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“…1998), specific for Lact. helveticus , and St4‐Cy3‐labelled probe specific for Streptococcus thermophilus (Mercier et al. 2000) have been used.…”

Section: Methodsmentioning

confidence: 99%

Natural whey starter for Parmigiano Reggiano: culture-independent approach

Bottari

Santarelli

Neviani

et al. 2010

Journal of Applied Microbiology

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Aims: The aim of this work was to obtain a deeper insight into the knowledge of microbial composition of Parmigiano Reggiano natural whey starters through different culture‐independent methods. Methods and Results: Eighteen different Parmigiano Reggiano natural whey starters sampled from three different provinces of this cheese production area and the nonacidified wheys from which they arose have been studied by length heterogeneity polymerase chain reaction (LH‐PCR) and fluorescent in situ hybridization (FISH). A high microbial composition variability between different samples has been observed. Conclusions: Revealing different images of the same community, LH‐PCR and FISH have given a more accurate view of the not well‐known Parmigiano Reggiano whey starter ecosystem. Significance and Impact of the Study: New lights have been shed on Parmigiano Reggiano natural whey starters microbial composition, highlighting how culture‐independent approach could be used and improved to study this and other food ecosystems.

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“…All three LysM sequences are required for AcmA to function optimally [69], but a single LysM suffices for PG binding [172]. AcmA also binds to PG in other bacteria, and even to the cells of different Gram-positive species in mixed communities [173]. …”

Section: Cell Wall Proteinsmentioning

confidence: 99%

Cell wall structure and function in lactic acid bacteria

2014

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The cell wall of Gram-positive bacteria is a complex assemblage of glycopolymers and proteins. It consists of a thick peptidoglycan sacculus that surrounds the cytoplasmic membrane and that is decorated with teichoic acids, polysaccharides, and proteins. It plays a major role in bacterial physiology since it maintains cell shape and integrity during growth and division; in addition, it acts as the interface between the bacterium and its environment. Lactic acid bacteria (LAB) are traditionally and widely used to ferment food, and they are also the subject of more and more research because of their potential health-related benefits. It is now recognized that understanding the composition, structure, and properties of LAB cell walls is a crucial part of developing technological and health applications using these bacteria. In this review, we examine the different components of the Gram-positive cell wall: peptidoglycan, teichoic acids, polysaccharides, and proteins. We present recent findings regarding the structure and function of these complex compounds, results that have emerged thanks to the tandem development of structural analysis and whole genome sequencing. Although general structures and biosynthesis pathways are conserved among Gram-positive bacteria, studies have revealed that LAB cell walls demonstrate unique properties; these studies have yielded some notable, fundamental, and novel findings. Given the potential of this research to contribute to future applied strategies, in our discussion of the role played by cell wall components in LAB physiology, we pay special attention to the mechanisms controlling bacterial autolysis, bacterial sensitivity to bacteriophages and the mechanisms underlying interactions between probiotic bacteria and their hosts.

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Effects of a muramidase on a mixed bacterial community

Cited by 22 publications

References 20 publications

Peptidoglycan ld-Carboxypeptidase Pgp2 Influences Campylobacter jejuni Helical Cell Shape and Pathogenic Properties and Provides the Substrate for the dl-Carboxypeptidase Pgp1

Peptidoglycan ld-Carboxypeptidase Pgp2 Influences Campylobacter jejuni Helical Cell Shape and Pathogenic Properties and Provides the Substrate for the dl-Carboxypeptidase Pgp1

Natural whey starter for Parmigiano Reggiano: culture-independent approach

Cell wall structure and function in lactic acid bacteria

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