Effects of a Monocomponent Protease on Performance Parameters and Protein Digestibility in Broiler Chickens

Kamel, N.F.; Naela,; M., Ragaa,; El-Banna, R.A.; Mohammed, Faten F.

doi:10.1016/j.aaspro.2015.08.062

Cited by 33 publications

(36 citation statements)

References 25 publications

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“…Protease supplementation also increases hydrolysis of antinutritional protein-origin factors such as trypsin and lectin inhibitors, enhancing the use efficiency of AA (Ghazi et al, 2002), even as supplied as "on top" (Fru-Nji et al, 2011;Kamel et al, 2015).…”

Section: Resultsmentioning

confidence: 99%

Metabolizable energy and digestible amino acids of full-fat soybean without or with protease supplementation in diets for broilers

et al. 2016

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Two experiments were carried out to determine the energy values and the true ileal digestibility of amino acids of nine toasted full-fat soybeans (FFSB), without or with the addition of monocomponent protease. In the first experiment, to determine the energy values, the method of excreta collection was used with 1120 broiler chicks at 14 days old, distributed in randomized blocks in a 10x2 factorial arrangement with nine full-fat soybeans (30% included) plus a control diet (70%) without or with the addition of protease, totaling 20 treatments with 8 replicates and 7 birds per cage. In the second experiment, 1120 broiler chickens from 24 to 28 days of age were distributed in randomized blocks in a 10x2 factor arrangement, with nine full-fat soybeans (40% included) + a protein free diet (60%), without or with the addition of protease, totaling 20 treatments with 8 replicates and 7 birds per cage. Ileal digesta collection was used to determine the true digestibility coefficients and the digestible amino acid content of full-fat soybean. The average values of nitrogen-corrected apparent metabolizable energy (AMEn) and their respective metabolization coefficients (CAMEn) were 3207 kcal/kg and 62.57%, respectively. The average values of digestibility coefficients of crude protein and essential and nonessential amino acids were 86.79, 87.90 and 84.34%, respectively. The inclusion of protease improved (P<0.05) all evaluated parameters. Therefore, its use is recommended in diets containing full-fat soybeans for broiler chickens.Index terms: Digestibility coefficients; metabolizability; monocomponent enzyme. RESUMOForam realizados dois experimentos para determinar os valores energéticos e os coeficientes de digestibilidade ileal verdadeiros de aminoácidos de nove sojas integrais tostadas, sem ou com a adição de protease monocomponente. No primeiro experimento, para a determinação dos valores energéticos, foi utilizado o método de coleta total de excretas, com 1120 pintos de corte com 14 dias de idade, distribuídos em blocos casualizados, em arranjo fatorial 10x2, com nove sojas integrais (30% de inclusão) + uma ração referência (70%), sem ou com adição de protease, totalizando 20 tratamentos, com 8 repetições e 7 aves por gaiola. No segundo experimento foram utilizados 1120 frangos dos 24 aos 28 dias de idade, distribuídos em blocos casualizados, em arranjo fatorial 10x2, com nove sojas integrais (40% de inclusão) + uma ração isenta de proteína (60%), sem ou com adição de protease, totalizando 20 tratamentos, com 8 repetições e 7 aves por gaiola. A coleta da digesta ileal foi utilizada para determinar os coeficientes de digestibilidade verdadeiros e o teor de aminoácidos digestíveis da soja integral. Os valores médios de energia metabolizável aparente corrigida para balanço de nitrogênio (EMAn) e o seu respectivo coeficiente de metabolizabilidade (CEMAn) foram de 3207 kcal/kg e 62,57%, respectivamente. Os valores médios dos coeficientes de digestibilidade da proteína bruta e dos aminoácidos, essenciais e não essen...

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Section: Resultsmentioning

confidence: 99%

Metabolizable energy and digestible amino acids of full-fat soybean without or with protease supplementation in diets for broilers

et al. 2016

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“…Researchers (Bedford & Schulze, ; Erdaw, Perez‐Maldonado, Bhuiyan, & Iji, ) suggested also that supplementation with microbial enzymes could reduce the negative impacts of ANFs in feedstuffs and thereby increase the bioavailability of nutrients (Martinez‐Amezcua, Parsons, & Baker, ), by reducing nutrient excretion (Adeola & Cowieson, ). Kamel, Ragaa, El‐Banna, and Mohamed () reported that supplementation with monocomponent protease improved protein digestibility. In a review, Nahm () concluded that supplementation of broiler diets with exogenous enzymes could reduce N excretion by up to 40%.…”

Section: Introductionmentioning

confidence: 99%

Physiological and health‐related response of broiler chickens fed diets containing raw, full‐fat soya bean meal supplemented with microbial protease

Erdaw

Perez-Maldonado²,

Iji

2017

Animal Physiology Nutrition

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A 2 × 3 factorial study (protease: 0 or 1,5000 PROT/kg and raw full-fat soya bean meal [RSBM] replacing the commercial SBM at 0, 45 and 75 g/kg of diet) was conducted to examine the performance of broilers. Phytase (2000 FYT/kg) was uniformly added to each diet, each also replicated six times, with eight birds per replicate. Birds were raised in climate-controlled rooms using sawdust as the bedding material and offered starter, grower and finisher diets. Feed intake (FI) and body weight gain (BWG) were reduced (p < .05) due to increasing levels of RSBM, but feed conversion ratio (FCR; 0-35 days) was unaffected. Over the first 24 days, neither RSBM nor protease supplementation affected (p > .05) mortality, footpad dermatitis or intestinal lesions in birds. At day 24, the weight, length, width and strength of tibia bone were reduced in chickens that received an elevated level of RSBM (75 g/kg of diet), but this was not significant at day 35. At day 24 (p < .05) and 35 (p < .01), Ca concentration in the litter was reduced when the RSBM level was increased in the diet, but P content was not affected. On days 24 (p < .05) and 35 (p < .01), the N content in litter was also increased with increase in dietary RSBM. Protease supplementation increased (p < .05) the uric acid concentration in the litter (at day 35), but the reverse was the case for ammonia concentration. Overall, the results of this study indicate that there are no major health-related risks, associated with the replacement of commercial SBM with RSBM (≤25%) in broiler diets.

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“…However, this fact did not occur in the present study, where the diets were based on corn, soybean meal and meat-and-bone meal. Kamel et al (2015) reported that the highest level of the major crystalline amino acids (methionine, lysine and threonine) provided in the diets associated with protease supplementation for broilers were the factors that most affected the carcass characteristics, regardless of metabolizable energy level and crude protein. This may led to an increase in the weight of the broiler's viscera, without altering the carcass yield and noble cuts.…”

Section: Resultsmentioning

confidence: 99%

“…Protease supplementation in diets for broilers is a relevant tool to improve the efficiency in utilizing vegetable and animal protein (Stefanello et al, 2016;Vieira et al, 2016;Cowieson et al, 2018). Protease promotes a higher degradation of antinutritional factors present in feedstuffs improves protein digestibility and decreases synthesis of endogenous enzymes, resulting in higher availability of amino acids for protein deposition (Angel et al, 2011;Kamel et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Protease Supplementation under Amino Acid Reduction in Diets Formulated with Different Nutritional Requirements for Broilers

Dessimoni

Dalólio

Moreira

et al. 2019

Braz. J. Poult. Sci.

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This study aimed to evaluate the effect of different reductions in digestible amino acids content (lysine, methionine, and threonine), according to two nutritional requirements in corn, soybean meal, and meat and bone meal based diets, with protease supplementation, on performance parameters and carcass characteristics. A total of 1080 day-old chicks, male, Cobb 500, were allotted to a completely randomized design, in a factorial arrangement 3 x 2, three reductions in digestible amino acids content (lysine, methionine, and threonine) and two nutritional requirements (Rostagno et al. 2005 and Cobb-Vantress Guidelines 2008), and all diets were supplemented with protease (200 ppm) with 6 replicates of 30 birds per pen. There was no significant interaction (p<0.05) between digestible amino acid reductions and both nutritional requirements for the performance variables and carcass yield and cuts. There was an effect of amino acid reduction and protease supplementation only on slaughter weight (p<0.05). Broilers fed according to the nutritional requirements of Rostagno et al. (2005) showed better (p<0.05) performance when compared to broilers fed as specified by the nutritional requirements of Cobb-Vantress (2008) with no significant differences in carcass characteristics. Protease supplementation of corn, soybean meal, and meat and bone meal based diets allows a reduction in the inclusion of crystalline amino acids (lysine, methionine, and threonine).

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Effects of a Monocomponent Protease on Performance Parameters and Protein Digestibility in Broiler Chickens

Cited by 33 publications

References 25 publications

Metabolizable energy and digestible amino acids of full-fat soybean without or with protease supplementation in diets for broilers

Metabolizable energy and digestible amino acids of full-fat soybean without or with protease supplementation in diets for broilers

Physiological and health‐related response of broiler chickens fed diets containing raw, full‐fat soya bean meal supplemented with microbial protease

Protease Supplementation under Amino Acid Reduction in Diets Formulated with Different Nutritional Requirements for Broilers

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