Effects of a mixture of low doses of atrazine and benzo[a]pyrene on the rat seminiferous epithelium either during or after the establishment of the blood-testis barrier in the rat seminiferous tubule culture model

Durand, Philippe; Blondet, Antonine; Martin, Guillaume; Carette, Diane; Pointis, Georges; Perrard, Marie-Hélène

doi:10.1016/j.tiv.2019.104699

Cited by 14 publications

(7 citation statements)

References 74 publications

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“…However, the decrease of the number of round spermatids was faster and more marked in the 8-day-than in the 20-22-day-old rat seminiferous tubule cultures. Hence, our study emphasizes the possible influence of the age of an individual on the effect of (a) toxicant(s) on spermatogenesis [32].…”

Section: We Then Tested Pesticides Selected On the Basis Of Their Per...mentioning

confidence: 76%

“…Similarly, by using Sertoli cell conditional Cx43 knock-out mice recent findings have reported that Cx43 is essential for control of Sertoli cell proliferation and differentiation [22,31]. Recently, we showed that our seminiferous tubule culture model, in bicameral chambers, allowed the settlement of the blood-testis barrier (BTB) in 8-day-old male rat cultures and the differentiation of spermatogonia into round spermatids [32]. Taken together, such data support the view that our co-culture systems enable to highlight mechanisms pertinent to the physiological processes.…”

Section: Scientific Validation Of These Systems (Physiology)mentioning

confidence: 87%

“…Our model can also respond to the need of some eco-toxycology studies. A large number of environmental factors can pollute air, water and food and may potentiate each other when present as a mixture [32,64,65]. Numerous studies suggest a decline in semen quality in some parts of the world [49,[66][67][68][69][70][71][72][73].…”

Section: Scientific Validation Of These Systems (Toxicology)mentioning

confidence: 99%

See 2 more Smart Citations

An In Vitro Method to Study Male Reproductive Toxicology: A Review on the Bio-AlteR® Technology Allowing a “Physio-Toxicology” Approach

2022

J Pharmacol Pharm Res

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Section: We Then Tested Pesticides Selected On the Basis Of Their Per...mentioning

confidence: 76%

Section: Scientific Validation Of These Systems (Physiology)mentioning

confidence: 87%

Section: Scientific Validation Of These Systems (Toxicology)mentioning

confidence: 99%

See 1 more Smart Citation

An In Vitro Method to Study Male Reproductive Toxicology: A Review on the Bio-AlteR® Technology Allowing a “Physio-Toxicology” Approach

2022

J Pharmacol Pharm Res

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“…Two blood-testis barrier proteins, Claudin-11 and Connexin 43, were impaired by treatment with a mixture of 1 μg/L streptozotocin and 1 μg/L BaP ( 93 ). The function of blood brain barrier was assessed by measuring the transendothelial electrical resistance of mouse brain microvascular endothelial cells, and the viability of cells was altered in the presence of BaP ( 94 ).…”

Section: Vascular Toxicity Effects and Underlying Mechanism Of Bapmentioning

confidence: 99%

Benzo(a)pyrene and cardiovascular diseases: An overview of pre-clinical studies focused on the underlying molecular mechanism

et al. 2022

Front. Nutr.

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Benzo(a)pyrene (BaP) is a highly toxic and carcinogenic polycyclic aromatic hydrocarbon (PAH) whose toxicological effects in the vessel-wall cells have been recognized. Many lines of evidence suggest that tobacco smoking and foodborne BaP exposure play a pivotal role in the dysfunctions of vessel-wall cells, such as vascular endothelial cell and vascular smooth muscle cells, which contribute to the formation and worsening of cardiovascular diseases (CVDs). To clarify the underlying molecular mechanism of BaP-evoked CVDs, the present study mainly focused on both cellular and animal reports whose keywords include BaP and atherosclerosis, abdominal aortic aneurysm, hypertension, or myocardial injury. This review demonstrated the aryl hydrocarbon receptor (AhR) and its relative signal transduction pathway exert a dominant role in the oxidative stress, inflammation response, and genetic toxicity of vessel-wall cells. Furthermore, antagonists and synergists of BaP are also discussed to better understand its mechanism of action on toxic pathways.

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“…A large number of environmental factors can pollute air, water and food and may potentiate each other when present as a mixture (Cotton et al 2016;Durand et al 2017Durand et al , 2020. Numerous studies suggest a decline in semen quality in some parts of the world (Carlsen et al 1992;Auger et al 1995;Adamopoulos et al 1996;Van Waeleghem et al 1996;Lackner et al 2005;Sripada et al 2007;Shine et al 2008;Mukhopadhyay et al 2010;Geoffroy-Siraudin et al 2012).…”

Section: Introductionmentioning

confidence: 99%

An in vitro bioassay to assess the potential global toxicity of waters on spermatogenesis: a pilot study

Blondet

Martin

Paulic

et al. 2021

Environ Sci Pollut Res

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Many toxicants are present in water as a mixture. Male infertility is one of the environmental impacts in developed countries. Using our rat seminiferous tubule culture model, we evaluated the effects of waters of different origins, on several parameters of the seminiferous epithelium. Concentrated culture medium was diluted with the waters to be tested (final concentrations of the tested waters were between 8 and 80%). The integrity of the blood-testis barrier was assessed by the trans-epithelial electric resistance (TEER). The levels of mRNAs specific of Sertoli cells, of cellular junctions, of each population of germ cells, of androgen receptor, of estrogen receptor α and of aromatase were also studied. We report, here, the results obtained with ten waters, some of them possessing a negative effect on spermatogenesis. The results showed that according to the tested waters, their effects on the parameters studied might be quite different indicating many different mechanisms of toxicity, including some endocrine disrupting effects. It has been reported that men with impaired semen parameters have an increased mortality rate suggesting semen quality may provide a fundamental biomarker of overall male health. Hence, we have developed a relevant in vitro bioassay allowing to evaluate the potential toxicity of different types of waters on male fertility and to assess some aspects of their mechanism of action. In addition to the TEER measure, the number and/or the identity of the studied mRNAs can be largely increased and/or modified, thus enhancing the possibility of using this model as a "warning system".

show abstract

Effects of a mixture of low doses of atrazine and benzo[a]pyrene on the rat seminiferous epithelium either during or after the establishment of the blood-testis barrier in the rat seminiferous tubule culture model

Cited by 14 publications

References 74 publications

An In Vitro Method to Study Male Reproductive Toxicology: A Review on the Bio-AlteR® Technology Allowing a “Physio-Toxicology” Approach

An In Vitro Method to Study Male Reproductive Toxicology: A Review on the Bio-AlteR® Technology Allowing a “Physio-Toxicology” Approach

Benzo(a)pyrene and cardiovascular diseases: An overview of pre-clinical studies focused on the underlying molecular mechanism

An in vitro bioassay to assess the potential global toxicity of waters on spermatogenesis: a pilot study

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