Effects of a Megasphaera elsdenii oral drench on reticulorumen pH dynamics in lactating dairy cows under subacute ruminal acidosis challenge

Mazon, Gustavo; Campler, Magnus R.; Holcomb, Chris; Bewley, J.M.; Costa, J.H.C.

doi:10.1016/j.anifeedsci.2020.114404

Cited by 10 publications

(7 citation statements)

References 19 publications

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“…In the rumen , LUB such as S. ruminantium and M. elsdenii can utilize lactic acid to produce VFA [ 29 ], and thus LUB-based DFMs are expected to aid in preventing the accumulation of lactate in the rumen and maintaining a higher ruminal pH as well as increasing VFA production. M. elsdenii is one of the species to prevent ruminal acidosis in dairy cows fed a high-concentrate diet, especially for the postpartum period [ 41 , 61 ]. This species seems to be the major lactic acid utilizer in the rumen when animals are fed readily fermentable carbohydrates, since other lactate-fermenting bacteria ( S. ruminantium ) undergo catabolite repression [ 62 , 63 ].…”

Section: Introductionmentioning

confidence: 99%

Implication and challenges of direct-fed microbial supplementation to improve ruminant production and health

Ban

Guan

2021

J Animal Sci Biotechnol

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Direct-fed microbials (DFMs) are feed additives containing live naturally existing microbes that can benefit animals’ health and production performance. Due to the banned or strictly limited prophylactic and growth promoting usage of antibiotics, DFMs have been considered as one of antimicrobial alternatives in livestock industry. Microorganisms used as DFMs for ruminants usually consist of bacteria including lactic acid producing bacteria, lactic acid utilizing bacteria and other bacterial groups, and fungi containing Saccharomyces and Aspergillus. To date, the available DFMs for ruminants have been largely based on their effects on improving the feed efficiency and ruminant productivity through enhancing the rumen function such as stabilizing ruminal pH, promoting ruminal fermentation and feed digestion. Recent research has shown emerging evidence that the DFMs may improve performance and health in young ruminants, however, these positive outcomes were not consistent among studies and the modes of action have not been clearly defined. This review summarizes the DFM studies conducted in ruminants in the last decade, aiming to provide the new knowledge on DFM supplementation strategies for various ruminant production stages, and to identify what are the potential barriers and challenges for current ruminant industry to adopt the DFMs. Overall literature research indicates that DFMs have the potential to mitigate ruminal acidosis, improve immune response and gut health, increase productivity (growth and milk production), and reduce methane emissions or fecal shedding of pathogens. More research is needed to explore the mode of action of specific DFMs in the gut of ruminants, and the optimal supplementation strategies to promote the development and efficiency of DFM products for ruminants.

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Section: Introductionmentioning

confidence: 99%

Implication and challenges of direct-fed microbial supplementation to improve ruminant production and health

Ban

Guan

2021

J Animal Sci Biotechnol

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“…This reduction could be attributed to the additional organic acid production potential that the finishing diet provided compared with the adaptation diet ( NASEM, 2016 ). As aforementioned, to the best of our knowledge, Mazon et al (2020) is the only study that reported daily DMI following MEG oral drench, but for a short period of time (7 to 10 d).…”

Section: Discussionmentioning

confidence: 84%

“…Conversely, no effects were observed when MEG was inoculated 1 d prior to the SARA challenge, indicating that in high-risk SARA animals, MEG should be administered at least 4 d prior to the challenge in order to improve DMI and production. These authors also reported that DMI was greater for MEG-administered animals 1 d following the SARA challenge, but only when MEG was inoculated 4 d prior to the challenge ( Mazon et al, 2020 ).…”

Section: Discussionmentioning

confidence: 97%

“…The lack of statistical significance on mean DMI for animals dosed with MEG corroborate with other studies in beef ( Drouillard et al, 2012 ; DeClerck et al, 2020a ; DeClerck et al, 2020b ; DeClerck et al, 2020c ) and dairy cattle ( Aikman et al, 2011 ; Henning et al, 2011 ). Evaluating a subacute ruminal acidosis ( SARA ) model, Mazon et al (2020) demonstrated that MEG administration 4 d prior to the challenge reduced the time-period and area at which rumen pH remained below 5.8 and 5.6, as well as improved DMI and milk production of mid-lactation dairy animals. Conversely, no effects were observed when MEG was inoculated 1 d prior to the SARA challenge, indicating that in high-risk SARA animals, MEG should be administered at least 4 d prior to the challenge in order to improve DMI and production.…”

Section: Discussionmentioning

confidence: 99%

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Effects of Megasphaera elsdenii administration on performance and carcass traits of finishing Bos indicus feedlot cattle

Lopes

Santos

Meschiatti

et al. 2021

Translational Animal Science

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This study evaluated the effects of Megasphaera elsdenii administration at the beginning of the feedlot period on performance of Bos taurus indicus bulls. On d 0, 383 Nellore bulls (initial shrunk body weight 384 ± 29.2 kg; initial age = 24 ± 2 mo) were assigned to treatments in a randomized complete block design. Treatments consisted of 1) 14 d adaptation diet and transition to a finishing diet (CONT), 2) CONT plus oral administration of 20 mL of Lactipro-NXT (M. elsdenii) on d 0 of the study (MEG-14), 3) CONT diet, consisting of 6 d of adaptation diet plus oral administration of 20 mL of Lactipro-NXT on d 0 of the study (MEG-6), and 4) No adaptation diet and oral administration of 20 mL of Lactipro-NXT on d 0 of the study (MEG-0). Experimental period lasted 119 d. No treatment effects were observed for any of the performance parameters evaluated herein (P ≥ 0.15). Nonetheless, a treatment × wk interaction was observed for DM, NEm, and NEg intakes (P < 0.0001). For all these parameters, MEG-0 and MEG-6 had a reduced intake vs. MEG-14 and CONT in the first wk of the study (P ≤ 0.05). For the carcass traits, no effects were observed for HCW (P ≥ 0.24), whereas MEG-6 had a greater REA when compared with MEG-0 and MEG-14 (quadratic effect; P = 0.04) and MEG-administered bulls tended to have a greater BFT vs. CONT (P = 0.08). In summary, M. elsdenii administration at the beginning of the feedlot period did not improve performance, whereas reducing the length of the adaptation period for 6 d improved REA of finishing Bos taurus indicus bulls.

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“…Since rumen microbiota is an integral part of feed digestion and nutrient metabolism, it is imperative to maintain an optimum population of multifarious rumen microbes (Liu et al 2021). When bacterial culture in the form of probiotic or directfed microbial (DFM) are supplemented, betterment in performance outcome and health status has been obtained through a better gut microbial balance (Mazon et al 2020;Mahesh et al 2021;Singh et al 2021).…”

mentioning

confidence: 99%

Effect of oral dosing of live and autoclaved culture of Ruminococcus flavefaciens FD-1 on rumen bacterial and fungal populations in Murrah buffaloes

Kumar¹,

Kumar²,

M³

et al. 2021

IJDS

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Dietary interventions aiming at increasing the number and activities of beneficial gut microbes could enhance digestive functions as well as the long-term welfare of dairy animals. In the present experiment, the effect of supplementation of a novel bacterial culture Ruminococcus flavefaciens FD-1 isolated from the rumen liquor of Murrah buffaloes was studied for its effect on rumen microbial populations enumerated using the most probable number technique. Three permanently fistulated buffaloes maintained on a high fibre diet were used as the source of rumen fluid for bacterial isolation that was subsequently tested in vivo. Twelve healthy mid-to-late lactating buffaloes in their second to third parity were divided into two similar groups of six each with a mean body weight of 601.5 kg. One group (LBC) was dosed with 300 mL of live bacterial culture, whereas an equal volume of autoclaved bacterial culture was dosed in another group (ABC) for a period of one month. The treatment-wise effect of supplementation of R. flavefaciens FD-1 culture was evident in an increase (P<0.05) in total rumen fungal population by 2.7 times in LBC than ABC in the post-dosing period. However, the period-wise comparison revealed that the population of bacteria augmented (P<0.05) by 2.16 and 3.17 times in both the groups ABC and LBC, respectively; whereas the magnitude of increase in fungal population was 5.04 and 10.3 times, respectively for both the groups. Post-dosing effects were evident for only group LBC in both bacteria and fungi, respectively increasing (P<0.05) the population by 2.16 and 8.28 times than pre-dosing period. These preliminary results may foster scope for developing species-specific (autochthonous) bacterial probiotic/direct-fed microbial based on R. flavefaciens FD-1 for large ruminants maintained on fibrous diets under tropical production systems.

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Effects of a Megasphaera elsdenii oral drench on reticulorumen pH dynamics in lactating dairy cows under subacute ruminal acidosis challenge

Cited by 10 publications

References 19 publications

Implication and challenges of direct-fed microbial supplementation to improve ruminant production and health

Implication and challenges of direct-fed microbial supplementation to improve ruminant production and health

Effects of Megasphaera elsdenii administration on performance and carcass traits of finishing Bos indicus feedlot cattle

Effect of oral dosing of live and autoclaved culture of Ruminococcus flavefaciens FD-1 on rumen bacterial and fungal populations in Murrah buffaloes

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