Effects of a functional agar surface on in vitro dentinogenesis induced in proteolytically isolated, agar-coated dental papillae in rat mandibular incisors

Kikuchi, Hiroshi; Sawada, Takashi; Yanagisawa, Takaaki

doi:10.1016/s0003-9969(96)00022-2

Cited by 19 publications

(36 citation statements)

References 56 publications

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“…The LLL interacts with different types of tissues and LLL therapy (LLLT) is known to cause varied reactions and modulate several biological processes. It has been demonstrated that the photo-stimulation mediated by the LLLT, in addition to increase ATP synthesis [6], produces an analgesic effect [7][8][9] and stimulates cell division [10], accelerating tissue healing [11][12][13]. Some specific laser types also have the capacity to stimulate the synthesis and deposition of collagen matrix by fibroblasts [14][15][16], and the increase of mitochondrial activity and cell metabolism [8] and proliferation [12].…”

Section: Introductionmentioning

confidence: 99%

Nutritional stress enhances cell viability of odontoblastlike cells subjected to low level laser irradiation

Tagliani¹,

Oliveira²,

Lins³

et al. 2010

Laser Phys. Lett.

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In spite of knowing that cells under stress are biostimulated by low level laser (LLL) irradiation, the ideal condition of stress to different cell lines has not yet been established. Consequently, the aim of the present in vitro study was to evaluate the effects of a defined parameter of LLL irradiation applied on stressed odontoblast-like pulp cells (MDPC-23). The cells were seeded (12500 cells/cm 2 ) in wells of 24-well plates using complete culture medium (DMEM) and incubated for 24 hours. Then, the DMEM was replaced by a new medium with low concentrations (nutritional stress condition) of fetal bovine serum (FBS) giving rise to the following experimental groups: G1: 2% FBS; G2: 5% FBS; and G3: 10% FBS. The cells were irradiated three times with LLL in specific parameters (808±3 nm, 100 mW, 1.5 J/cm 2 ) every 24 hours. No irradiation was carried out in groups G4 (2% FBS-Control), G5 (5% FBS-Control), and G6 (10% FBS-Control). For all groups, the cell metabolism (MTT assay) and morphology (SEM) was evaluated. The experimental groups showed enhanced cell metabolism and normal cell morphology regardless of FBS concentration. A slight increase in the cell metabolism was observed only in group G2. It was concluded that cell nutritional stress caused by reducing the concentration of FBS to 5% is the most suitable method to assess the biostimulation of LLL irradiated MDPC-23 cells.MDPC-23 cells adhered to the glass substrate exhibited a spindle-shaped morphology and some mitosis were observed (arrows); SEM ×500

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Section: Introductionmentioning

confidence: 99%

Nutritional stress enhances cell viability of odontoblastlike cells subjected to low level laser irradiation

Tagliani¹,

Oliveira²,

Lins³

et al. 2010

Laser Phys. Lett.

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“…Several studies have demonstrated that biostimulation by low-level laser therapy (LLLT) [1][2][3][4] in addition to increase ATP synthesis [5] also produces an analgesic effect [6], acceleration of tissue healing [7,8], triggering of acid nucleic production and increase of cell division, proliferation and metabolism [8][9][10]. Some specific types of laser have also the capacity to stimulate the synthesis and deposition of collagen matrix by fibroblasts [11][12][13] due to the primary action of infrared light on lysosomes and mitochondria [14].…”

Section: Introductionmentioning

confidence: 99%

“…Under physiological conditions, these cells synthesize proteins typical of odontoblasts, such as sialoprotein and phosphorine, which are associated to alkaline phosphatase (ALP) activity and dentin matrix protein synthesis (particularly collagen type I) [10,30,31]. Some immortalized cultures of odontoblast-like cell lines are effectively described in the literature [24,30,32,33].…”

Section: Introductionmentioning

confidence: 99%

Effect of low-level laser irradiation on odontoblast-like cells

et al. 2008

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Low-level laser therapy (LLLT), also referred to as therapeutic laser, has been recommended for a wide array of clinical procedures, among which the treatment of dentinal hypersensitivity. However, the mechanism that guides this process remains unknown. Therefore, the objective of this study was to evaluate in vitro the effects of LLL irradiation on cell metabolism (MTT assay), alkaline phosphatase (ALP) expression and total protein synthesis. The expression of genes that encode for collagen type-1 (Col-1) and fibronectin (FN) was analyzed by RT-PCR. For such purposes, odontoblast-like cell line (MDPC-23) was previously cultured in Petri dishes (15000 cells/cm 2 ) and submitted to stress conditions during 12 h. Thereafter, 6 applications with a monochromatic near infrared radiation (GaAlAs) set at predetermined parameters were performed at 12-h intervals. Nonirradiated cells served as a control group. Neither the MTT values nor the total protein levels of the irradiated group differed significantly from those of the control group (Mann-Whitney test; p > 0.05). On the other hand, the irradiated cells showed a decrease in ALP activity (Mann-Whitney test; p < 0.05). RT-PCR results demonstrated a trend to a specific reduction in gene expression after cell irradiation, though not significant statistically (Mann-Whitney test; p > 0.05). It may be concluded that, under the tested conditions, the LLLT parameters used in the present study did not influence cell metabolism, but reduced slightly the expression of some specific proteins.

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“…1 LLLT using far-red to near-infrared (NIR) spectra has been found to modulate different biological processes in cell cultures such as: increasing ATP synthesis, 2 producing analgesic effects, 3 accelerating tissue healing, 4,5 and triggering acid nucleic production, increasing cell proliferation and metabolism. [5][6][7] Some specific types of laser can also stimulate fibroblasts to synthesize and deposit collagen matrix. [8][9][10] A number of in vitro investigations using a wide range of cell cultures have provided different data concerning cell proliferation 11,12 and differentiation, 13 DNA and bone protein synthesis, 1,[14][15][16] and bone tissue formation without genotoxic or cytotoxic damage.…”

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confidence: 99%

A Novel 785-nm Laser Diode-Based System for Standardization of Cell Culture Irradiation

Lins

Oliveira

Guimarães

et al. 2013

Photomedicine and Laser Surgery

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Objective: The purpose of this study was to develop a novel device that concatenates alignment of infrared lasers and parallel procedure of irradiation. The purpose of this is to seek standardization of in vitro cell irradiation, which allows analysis and credible comparisons between outcomes of different experiments. Background data: Experimental data obtained from infrared laser therapies have been strongly dependent upon the irradiation setup. Although further optical alignment is difficult to achieve, in contact irradiation it usually occurs. Moreover, these methods eventually use laser in a serial procedure, extending the time to irradiate experimental samples. Methods: A LASERTable (LT) device was designed to provide similar infrared laser irradiation in 12 wells of a 24 well test plate. It irradiated each well by expanding the laser beam until it covers the well bottom, as occurs with unexpanded irradiation. To evaluate the effectiveness of this device, the spatial distribution of radiation was measured, and the heating of plain culture medium was monitored during the LT operation. The irradiation of LT (up to 25 J/cm 2 -20 mW/cm 2 ; 1.250 sec) was assessed on odontoblast-like cells adhered to the bottom of wells containing 1 mL of plain culture medium. Cell morphology and metabolism were also evaluated. Results: Irradiation with LT presented a Gaussian-like profile when the culture medium was not heated > 1°C. It was also observed that the LT made it 10 times faster to perform the experiment than did serial laser irradiation. In addition, the data of this study revealed that the odontoblast-like cells exposed to low-level laser therapy (LLLT) using the LT presented higher metabolism and normal morphology. Conclusions: The experimental LASERTable assessed in this study provided parameters for standardization of infrared cell irradiation, minimizing the time spent to irradiate all samples. Therefore, this device is a helpful tool that can be effectively used to evaluate experimental LLLT protocols.

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Effects of a functional agar surface on in vitro dentinogenesis induced in proteolytically isolated, agar-coated dental papillae in rat mandibular incisors

Cited by 19 publications

References 56 publications

Nutritional stress enhances cell viability of odontoblastlike cells subjected to low level laser irradiation

Nutritional stress enhances cell viability of odontoblastlike cells subjected to low level laser irradiation

Effect of low-level laser irradiation on odontoblast-like cells

A Novel 785-nm Laser Diode-Based System for Standardization of Cell Culture Irradiation

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