Objectives: To investigate whether docosahexaenoic acid (DHA) supplementation was able to ameliorate attention-deficit/ hyperactivity disorder(AD/HD) symptoms in AD/HD children. Design and subjects: A placebo-controlled double-blind study with 40 AD/HD (including eight AD/HD-suspected) children of 6-12 y of age who were mostly without medication. Subjects of a DHA group (n ¼ 20) took active foods containing fish oil (fermented soybean milk, bread rolls and steamed bread; 3.6 g DHA/week from these foods) for 2 months, whereas those of a control group (n ¼ 20) took indistinguishable control foods without fish oil. The following items were measured at the start and end of the study: (1) attention deficit, hyperactivity and impulsivity (AD/HD-related symptoms according to DSM-IV criteria); (2) aggression assessed by both parents and teachers; (3) visual perception (finding symbols out of a table); (4) visual and auditory short-term memory; (5) development of visual-motor integration; (6) continuous performance; (7) impatience. Results: Changes in tests 1, 2, 3, 5 and 7 over time did not significantly differ between the two groups. However, visual shortterm memory and errors of commission (continuous performance) significantly improved in the control group compared with the changes over time in the DHA group (P ¼ 0.02 and 0.001, respectively). Recalculation without AD/HD-suspected subjects (n ¼ 4 each group) showed similar P-values with regard to both measures. Conclusion: DHA supplementation did not improve AD/HD-related symptoms. Treatment of ADHD with fatty acids deserves further investigation, but careful attention should be paid as to which fatty acid(s) is used.