Effects of a dental adhesive incorporating antibacterial monomer on the growth, adherence and membrane integrity of Streptococcus mutans

Li, Fang; Chen, Ji Hua; Chai, Zhiguo; Zhang, Ling; Xiao, Yuhong; Fang, Ming; Ma, Sai

doi:10.1016/j.jdent.2008.12.004

Cited by 167 publications

(192 citation statements)

References 28 publications

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“…It is desirable for bonding agents to be antibacterial, to inhibit recurrent caries at the toothcomposite margins (Imazato, 2003;Imazato et al, 2007;Li et al, 2009). Dentin primer has direct contact with tooth structure; hence it would be beneficial to use antibacterial primers.…”

Section: Introduction Cmentioning

confidence: 99%

Anti-biofilm Dentin Primer with Quaternary Ammonium and Silver Nanoparticles

et al. 2012

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Antibacterial bonding agents could combat recurrent caries at the tooth-composite margins. The objectives of this study were to develop novel antibacterial dentin primers containing quaternary ammonium dimethacrylate (QADM) and nanoparticles of silver (NAg), and to investigate the effects on dentin bond strength and dental plaque microcosm biofilms for the first time. Scotchbond Multi-Purpose ("SBMP") bonding agent was used. QADM and NAg were incorporated into SBMP primer, yielding 4 primers: SBMP primer (control), control + 10% QADM (mass), control + 0.05% NAg, and control + 10% QADM + 0.05% NAg. Human saliva was collected to grow microcosm biofilms. The NAg particle size (mean ± SD; n = 100) was 2.7 ± 0.6 nm. Dentin shear bond strengths (n = 10) with human third molars were approximately 30 MPa for all groups (p > 0.1). QADMNAg-containing primer increased the bacteria inhibition zone by 9-fold, compared with control primer (p < 0.05). QADM-NAg-containing primer reduced lactic acid production and colony-forming units of total micro-organisms, total streptococci, and mutans streptococci by an order of magnitude. In conclusion, novel QADM-NAg-containing primers were strongly antibacterial without compromising dentin bond strength, and hence are promising to inhibit biofilms and secondary caries. The processing method of incorporating QADM and NAg together into the same primer produced the strongest antibacterial effect, which could have a wide applicability to other bonding systems.KEY WOrDs: antibacterial dentin primer, quaternary ammonium salt, dental biomaterials, bond strength, dental plaque microcosm biofilm, caries inhibition.

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Section: Introduction Cmentioning

confidence: 99%

Anti-biofilm Dentin Primer with Quaternary Ammonium and Silver Nanoparticles

et al. 2012

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“…Biofilm acids could lead to caries (ten Cate, 2006); hence antibacterial resins were developed to inhibit biofilms (Imazato et al, 1999;Imazato, 2003;Beyth et al, 2006;Namba et al, 2009;Cheng et al, 2012a). Indeed, previous studies investigated the copolymerization of quaternary ammonium methacrylates (QAMs) to develop antibacterial resins (Imazato, 2003;Li et al, 2009;Xie et al, 2011;Antonucci et al, 2012;Xu et al, 2012;Zhang et al, 2012).Composites are the principal materials for tooth-cavity restorations (Ferracane, 2011) which are bonded in cavities via adhesives (Ferracane et al, 2010;Spencer et al, 2010;Pashley et al, 2011). Residual bacteria could exist in the prepared cavity, and microleakage could allow bacteria to invade the tooth-restoration interfaces.…”

mentioning

confidence: 99%

Effects of Quaternary Ammonium Chain Length on Antibacterial Bonding Agents

2013

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The objectives of this study were to synthesize new quaternary ammonium methacrylates (QAMs) with systematically varied alkyl chain lengths (CL) and to investigate, for the first time, the CL effects on antibacterial efficacy, cytotoxicity, and dentin bond strength of bonding agents. QAMs were synthesized with CL of 3 to 18 and incorporated into Scotchbond Multi-Purpose (SBMP) bonding agent. The cured resins were inoculated with Streptococcus mutans. Bacterial early attachment was investigated at 4 hrs. Biofilm colonyforming units (CFU) were measured after 2 days. With CL increasing from 3 to 16, the minimum inhibitory concentration and minimum bactericidal concentration were decreased by 5 orders of magnitude. Incorporating QAMs into SBMP reduced bacterial early attachment, with the least colonization at CL = 16. Biofilm CFU for CL = 16 was 4 log lower than SBMP control (p < .05). All groups had similar dentin bond strengths (p > .1). The new antibacterial materials had fibroblast/ odontoblast viability similar to that of commercial controls. In conclusion, increasing the chain length of new QAMs in bonding agents greatly increased the antibacterial efficacy. A reduction in Streptococcus mutans biofilm CFU by 4 log could be achieved, without compromising bond strength and cytotoxicity. New QAM-containing bonding agents are promising for a wide range of restorations to inhibit biofilms.KEY WOrDs: antibacterial activity, Streptococcus mutans, quaternary ammonium compounds, dentin bonding, fibroblasts, odontoblasts. (Mjör and Toffeneti, 2000;Sakaguchi, 2005). Replacing failed restorations accounts for 50% to 70% of all restorations performed (Frost, 2002;Bagramian et al., 2009). Biofilm acids could lead to caries (ten Cate, 2006); hence antibacterial resins were developed to inhibit biofilms (Imazato et al., 1999;Imazato, 2003;Beyth et al., 2006;Namba et al., 2009;Cheng et al., 2012a). Indeed, previous studies investigated the copolymerization of quaternary ammonium methacrylates (QAMs) to develop antibacterial resins (Imazato, 2003;Li et al., 2009;Xie et al., 2011;Antonucci et al., 2012;Xu et al., 2012;Zhang et al., 2012).Composites are the principal materials for tooth-cavity restorations (Ferracane, 2011) which are bonded in cavities via adhesives (Ferracane et al., 2010;Spencer et al., 2010;Pashley et al., 2011). Residual bacteria could exist in the prepared cavity, and microleakage could allow bacteria to invade the tooth-restoration interfaces. Therefore, it is beneficial for primer/ adhesive to be antibacterial (Imazato et al., 1998;Imazato, 2003;Li et al., 2009;Hiraishi et al., 2010;Cheng et al., 2012b). Quaternary ammonium can cause bacteria lysis by binding to membranes (Beyth et al., 2006). A previous study revealed that long polymeric chains with positive charges and hydrophobicity effectively killed bacteria (Lin et al., 2002). When the alkyl chain length (CL) was increased, the hydrophobicity increased, thereby enhancing its propensity to penetrate the hydrophobic bacterial membrane (Tiller...

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“…Furthermore, the relative level of gtf B and gtf C expression by S. mutans in the biofilm was quantified by real-time reversetranscription polymerase chain-reaction and found that they were suppressed. Therefore, modified adhesive could decrease the cariogenicity of S. mutans 51) . In addition, the cytotoxicity of modified adhesive eluents were tested with 3-(4, 5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide cleavage assay (MTT assay).…”

Section: Adhesive Systemmentioning

confidence: 99%

“…The antibacterial effect against two E. faecalis strains (ATCC and an endodontic isolate) were assessed, and results had shown that addition of QPEI nanoparticles improved the killing ability of PCS against biofilms of both E. faecalis strains and the effects of AH Plus on the biomass of biofilms from the ATCC strain. Moreover, there were no relevant changes in physicochemical and 95) quaternary ammonium polyethyleneimine QPEI unpolymerizable composite resin 24) , endodontic materials [89][90][91] quaternary ammonium dimethacrylate QADM/IMQ polymerizable composite resin [25][26][27]32) , adhesive system 42) 2-methacryloxylethyl dodecyl methyl ammonium bromide MAE-DB polymerizable composite resin 28,29) 2-methacryloxylethyl hexadecyl methyl ammonium bromide MAE-HB polymerizable composite resin 28,29) 2-Dimethyl-2-dodecyl-1-methacryloxyethyl ammonium iodine DDMAI polymerizable composite resin 30) quaternary ammonium methacrylate QAM polymerizable composite resin 31) , adhesive system 5,53,54) 2-methacryloyloxyethyl phosphorylcholine MPC polymerizable composite resin 33) dimethylaminododecyl methacrylate DMADDM polymerizable adhesive system [43][44][45] quaternary ammonium methacrylate polymer QAMP unpolymerizable adhesive system [46][47][48] methacryloxylethyl cetyl ammonium chloride DMAE-CB polymerizable adhesive system [49][50][51][52] benzalkonium chloride BAC unpolymerizable adhesive system [55][56][57] , glass ionomer cement 73,74) , endodontic materials 87) poly-2-methacry...…”

Section: Endodontic Materialsmentioning

confidence: 99%

Quaternary ammonium compounds in dental restorative materials

Zhang

Chen

et al. 2018

Dent. Mater. J.

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Quaternary ammonium compounds (QACs) have been used widely in medicine, owing to their antimicrobial properties. They also have strong permeability, stable performance, low skin irritation, low toxicity, low corrosion, lasting biological effects and so on, comparing with other antimicrobial agents. At the end of last century, polymerizable quaternary ammonium antimicrobial monomers had been synthesized and applied in dentistry, in order to reduce or prevent microbial biofilm formation on dental materials surfaces. This review aims to discuss the current progress of QACs applied in composite resin, adhesive systems, acrylic resin, glass ionomer cement and endodontic materials, regarding to their antimicrobial potential, mechanical properties, and biocompatibility.

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Effects of a dental adhesive incorporating antibacterial monomer on the growth, adherence and membrane integrity of Streptococcus mutans

Cited by 167 publications

References 28 publications

Anti-biofilm Dentin Primer with Quaternary Ammonium and Silver Nanoparticles

Anti-biofilm Dentin Primer with Quaternary Ammonium and Silver Nanoparticles

Effects of Quaternary Ammonium Chain Length on Antibacterial Bonding Agents

Quaternary ammonium compounds in dental restorative materials

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