Effects of a dairy–yeast prebiotic and water hardness on the growth performance, mineral composition and gut microflora of fathead minnow (Pimephales promelas) in recirculating systems

Lochmann, Rebecca; Phillips, Harold; Xie, Lei

doi:10.1016/j.aquaculture.2011.08.004

Cited by 12 publications

(8 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Both technical differences (cultured vs. sequenced organisms, clones vs. short amplicons, choice of variable region sequenced, primer bias, sequencing depth, analysis) as well as differences related to the fish (age, diet, fresh caught vs. domesticated, water quality, husbandry, habitat) limit the ability to make direct comparisons of relative abundances across studies of other freshwater omnivorous fish gut microbiomes. Despite these caveats, these fathead minnow core microbiome OTUs are generally similar to the most common genera reported previously for zebrafish [ 23 ], the common carp [ 54 ] (both also family Cyprinidae), other freshwater omnivorous fish [ 24 , 25 , 80 ], and, in a limited fashion, for the fathead minnow [ 27 ]. There are also some differences between the fathead minnow core microbiome and that of other related fishes.…”

Section: Discussionsupporting

confidence: 76%

“…The eight co-housed baseline day 0 samples show low inter-individual variation in microbial community composition (mean pairwise Pearson r = 0.80), as estimated based on the relative abundance of 16S rRNA gene sequences observed. Among the most abundant taxa inferred in the fathead minnow gut are many bacteria previously observed in other freshwater fish [ 23 - 27 ]: Aeromonadaceae (mean abundance: 8.5% baseline, 57.5% day 7 to 21 control), Shewanellaceae (mean abundance: 2.2% baseline, 9.1% day 7 to 21 control), as well as Flavobacteriaceae, Fusobacteriaceae , Deefgea , Pseudomonadaceae , Plesiomonas , and Cetobacterium . Some of these taxa are included in the set of 20 OTUs that were present across at least 95% of the 103 sampled fish across all time points and exposures, representing a potential fathead minnow ‘core’ microbiome (Table 2 ; Additional file 5 : Figure S5).…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Perturbation and restoration of the fathead minnow gut microbiome after low-level triclosan exposure

et al. 2015

View full text Add to dashboard Cite

BackgroundTriclosan is a widely used antimicrobial compound and emerging environmental contaminant. Although the role of the gut microbiome in health and disease is increasingly well established, the interaction between environmental contaminants and host microbiome is largely unexplored, with unknown consequences for host health. This study examined the effects of low, environmentally relevant levels of triclosan exposure on the fish gut microbiome. Developing fathead minnows (Pimephales promelas) were exposed to two low levels of triclosan over a 7-day exposure. Fish gastrointestinal tracts from exposed and control fish were harvested at four time points: immediately preceding and following the 7-day exposure and after 1 and 2 weeks of depuration.ResultsA total of 103 fish gut bacterial communities were characterized by high-throughput sequencing and analysis of the V3-V4 region of the 16S rRNA gene. By measures of both alpha and beta diversity, gut microbial communities were significantly differentiated by exposure history immediately following triclosan exposure. After 2 weeks of depuration, these differences disappear. Independent of exposure history, communities were also significantly structured by time. This first detailed census of the fathead minnow gut microbiome shows a bacterial community that is similar in composition to those of zebrafish and other freshwater fish. Among the triclosan-resilient members of this host-associated community are taxa associated with denitrification in wastewater treatment, taxa potentially able to degrade triclosan, and taxa from an unstudied host-associated candidate division.ConclusionsThe fathead minnow gut microbiome is rapidly and significantly altered by exposure to low, environmentally relevant levels of triclosan, yet largely recovers from this short-term perturbation over an equivalently brief time span. These results suggest that even low-level environmental exposure to a common antimicrobial compound can induce significant short-term changes to the gut microbiome, followed by restoration, demonstrating both the sensitivity and resilience of the gut flora to challenges by environmental toxicants. This short-term disruption in a developing organism may have important long-term consequences for host health. The identification of multiple taxa not often reported in the fish gut suggests that microbial nitrogen metabolism in the fish gut may be more complex than previously appreciated.Electronic supplementary materialThe online version of this article (doi:10.1186/s40168-015-0069-6) contains supplementary material, which is available to authorized users.

show abstract

Section: Discussionsupporting

confidence: 76%

Section: Resultsmentioning

confidence: 99%

Perturbation and restoration of the fathead minnow gut microbiome after low-level triclosan exposure

et al. 2015

View full text Add to dashboard Cite

show abstract

“…Lochmann et al . () investigated the effect of GroBiotic ® ‐A (consisting of a mixture of partially autolysed brewer's yeast, dairy ingredient components and dried fermentation products) (0 and 20 g kg −1 ) on gut microbiota of fathead minnow ( Pimephales promelas ) in recirculating systems with low or moderate water hardness. Only Acinetobacter spp.…”

Section: Effect Of Prebioticsmentioning

confidence: 99%

Effect of dietary components on the gut microbiota of aquatic animals. A never‐ending story?

et al. 2015

View full text Add to dashboard Cite

It is well known that healthy gut microbiota is essential to promote host health and well‐being. The intestinal microbiota of endothermic animals as well as fish are classified as autochthonous or indigenous, when they are able to colonize the host's epithelial surface or are associated with the microvilli, or as allochthonous or transient (associated with digesta or are present in the lumen). Furthermore, the gut microbiota of aquatic animals is more fluidic than that of terrestrial vertebrates and is highly sensitive to dietary changes. In fish, it is demonstrated that [a] dietary form (live feeds or pelleted diets), [b] dietary lipid (lipid levels, lipid sources and polyunsaturated fatty acids), [c] protein sources (soybean meal, krill meal and other meal products), [d] functional glycomic ingredients (chitin and cellulose), [e] nutraceuticals (probiotics, prebiotics, synbiotics and immunostimulants), [f] antibiotics, [g] dietary iron and [h] chromic oxide affect the gut microbiota. Furthermore, some information is available on bacterial colonization of the gut enterocyte surface as a result of dietary manipulation which indicates that changes in indigenous microbial populations may have repercussion on secondary host–microbe interactions. The effect of dietary components on the gut microbiota is important to investigate, as the gastrointestinal tract has been suggested as one of the major routes of infection in fish. Possible interactions between dietary components and the protective microbiota colonizing the digestive tract are discussed.

show abstract

“…Najveća je koncentracija kalcijuma, zbog njegove zastupljenosti u Zemljinoj kori u obliku minerala kalcita, dolomita, i dr. [5,24]. Tvrdoća vode je određivana kompleksometrijskom metodom.…”

Section: Slika 4 -Satelitski Snimak Pogona Filtracije Rudnika Bakra Munclassified

pH value and concentrations of total dry residue and suspended matter in the wastewater of the Majdanpek Copper mine

Serbula¹,

Ristić²,

Manasijević³

et al. 2014

Zas Mat

View full text Add to dashboard Cite

show abstract

Effects of a dairy–yeast prebiotic and water hardness on the growth performance, mineral composition and gut microflora of fathead minnow (Pimephales promelas) in recirculating systems

Cited by 12 publications

References 30 publications

Perturbation and restoration of the fathead minnow gut microbiome after low-level triclosan exposure

Perturbation and restoration of the fathead minnow gut microbiome after low-level triclosan exposure

Effect of dietary components on the gut microbiota of aquatic animals. A never‐ending story?

pH value and concentrations of total dry residue and suspended matter in the wastewater of the Majdanpek Copper mine

Contact Info

Product

Resources

About