Effects of a combined mechanical stimulation protocol: Value for skeletal muscle tissue engineering

Boonen, Kjm Kristel; Langelaan, Mlp Marloes; Polak, RB Roderick; Schaft, Dwj Daisy van der; Baaijens, Fpt Frank; Post, MJ Mark

doi:10.1016/j.jbiomech.2010.01.039

Cited by 88 publications

(79 citation statements)

References 25 publications

(32 reference statements)

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“…We chose to work with a combination of collagen and matrigel, because collagen allows for cell alignment and because the myoblast progenitor cells require the presence of basement membrane derived proteins as determined in previous 2D studies 12 . Moreover, fibrin gels have been tested in our laboratory and seem not to support the C2C12 and myoblast progenitor cells as does the mixture of collagen and Matrigel™ and especially do not lead to tissue compaction 14 . The model as described here has been used already in studies on pressure ulcer damage while using a cell line 15 .…”

Section: Discussionmentioning

confidence: 99%

Engineering Skeletal Muscle Tissues from Murine Myoblast Progenitor Cells and Application of Electrical Stimulation

Schaft

Spreeuwel

Boonen

et al. 2013

JoVE

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Engineered muscle tissues can be used for several different purposes, which include the production of tissues for use as a disease model in vitro, e.g. to study pressure ulcers, for regenerative medicine and as a meat alternative 1 . The first reported 3D muscle constructs have been made many years ago and pioneers in the field are Vandenburgh and colleagues 2,3 . Advances made in muscle tissue engineering are not only the result from the vast gain in knowledge of biochemical factors, stem cells and progenitor cells, but are in particular based on insights gained by researchers that physical factors play essential roles in the control of cell behavior and tissue development. State-of-the-art engineered muscle constructs currently consist of cell-populated hydrogel constructs. In our lab these generally consist of murine myoblast progenitor cells, isolated from murine hind limb muscles or a murine myoblast cell line C2C12, mixed with a mixture of collagen/Matrigel and plated between two anchoring points, mimicking the muscle ligaments. Other cells may be considered as well, e.g. alternative cell lines such as L6 rat myoblasts 4

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Section: Discussionmentioning

confidence: 99%

Engineering Skeletal Muscle Tissues from Murine Myoblast Progenitor Cells and Application of Electrical Stimulation

Schaft

Spreeuwel

Boonen

et al. 2013

JoVE

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“…Mechanical conditioning, or stretch, is an important physiological stimulus that has been applied to skeletal myofiber cultures to mimic in vivo exercise (4,23,26,34). The duration and percentage of strain modulate the effects on muscle in vitro: 10% strain at 1 Hz for 1 h alternated with 23 h of relaxation effectively inhibited differentiation and promoted myoblast proliferation (18), while 10% strain at 0.5 Hz for 1 h alternated with 5 h of relaxation induced myoblast differentiation (45).…”

mentioning

confidence: 99%

Conditions that promote primary human skeletal myoblast culture and muscle differentiation in vitro

Cheng

El-Abd

Bui

et al. 2014

American Journal of Physiology-Cell Physiology

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Conditions under which skeletal myoblasts are cultured in vitro are critical to growth and differentiation of these cells into mature skeletal myofibers. We examined several culture conditions that promoted human skeletal myoblast (HSkM) culture and examined the effect of microRNAs and mechanical stimulation on differentiation. Culture conditions for HSkM are different from those that enable rapid C2C12 myoblast differentiation. Culture on a growth factor-reduced Matrigel (GFR-MG)-coated surface in 2% equine serum-supplemented differentiation medium to promote HSkM differentiation under static conditions was compared with culture conditions used for C2C12 cell differentiation. Such conditions led to a Ͼ20-fold increase in myogenic miR-1, miR-133a, and miR-206 expression, a Ͼ2-fold increase in myogenic transcription factor Mef-2C expression, and an increase in sarcomeric ␣-actinin protein. Imposing Ϯ10% cyclic stretch at 0.5 Hz for 1 h followed by 5 h of rest over 2 wk produced a Ͼ20% increase in miR-1, miR-133a, and miR-206 expression in 8% equine serum and a Ͼ35% decrease in 2% equine serum relative to static conditions. HSkM differentiation was accelerated in vitro by inhibition of proliferationpromoting miR-133a: immunofluorescence for sarcomeric ␣-actinin exhibited accelerated development of striations compared with the corresponding negative control, and Western blotting showed 30% more ␣-actinin at day 6 postdifferentiation. This study showed that 100 g/ml GFR-MG coating and 2% equine serum-supplemented differentiation medium enhanced HSkM differentiation and myogenic miR expression and that addition of antisense miR-133a alone can accelerate primary human skeletal muscle differentiation in vitro. skeletal muscle; microRNA; myogenesis; differentiation; tissue engineering THE DYNAMICS OF PRIMARY HUMAN skeletal myoblast (HSkM) growth and differentiation into mature myofibers are critical to their use for applications in regenerative medicine, such as repair of severe muscle injuries, congenital defects, and muscular dystrophy. Most studies have focused on rodent myoblasts, and the bulk of the research has been carried out with the widely used immortalized murine C2C12 myoblast line, which is used for ease of culture, differentiation potential, and accessibility (7). Culture conditions for these cells have been optimized to ensure rapid growth and effective differentiation in vitro. These cells do not require protein-coated substrates and differentiate well in a range of equine serum-supplemented differentiation media (DM) (14,24,28,29,32). While C2C12 cells have provided invaluable information about key mechanistic steps in differentiation (31), extensive in vitro cultivation may lead to deviations from normal biological processes that are important for differentiation of myoblasts in a normal in vivo environment. Less is known about the dynamics of key differentiation factors in primary human myoblasts and how culture conditions affect the changes in these factors (3).In vitro, rodent myoblast differentiati...

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“…The methods currently used for engineering such muscle-mimetic structures in vitro range from scaffold anchoring [41,125] or micropatterning of biomaterials to apply myotube formation [126 -128] to mechanical or electrical stimulation within bioreactors in two-dimensional or 3D settings [129][130][131][132][133][134][135][136].…”

Section: Hydrogels For In Vitro Engineering Skeletal Muscle Tissuesmentioning

confidence: 99%

Hydrogel biomaterials and their therapeutic potential for muscle injuries and muscular dystrophies

Lev¹,

Seliktar²

2018

J. R. Soc. Interface.

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Muscular diseases such as muscular dystrophies and muscle injuries constitute a large group of ailments that manifest as muscle weakness, atrophy or fibrosis. Although cell therapy is a promising treatment option, the delivery and retention of cells in the muscle is difficult and prevents sustained regeneration needed for adequate functional improvements. Various types of biomaterials with different physical and chemical properties have been developed to improve the delivery of cells and/or growth factors for treating muscle injuries. Hydrogels are a family of materials with distinct advantages for use as cell delivery systems in muscle injuries and ailments, including their mild processing conditions, their similarities to natural tissue extracellular matrix, and their ability to be delivered with less invasive approaches. Moreover, hydrogels can be made to completely degrade in the body, leaving behind their biological payload in a process that can enhance the therapeutic process. For these reasons, hydrogels have shown great potential as cell delivery matrices. This paper reviews a few of the hydrogel systems currently being applied together with cell therapy and/or growth factor delivery to promote the therapeutic repair of muscle injuries and muscle wasting diseases such as muscular dystrophies.

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Effects of a combined mechanical stimulation protocol: Value for skeletal muscle tissue engineering

Cited by 88 publications

References 25 publications

Engineering Skeletal Muscle Tissues from Murine Myoblast Progenitor Cells and Application of Electrical Stimulation

Engineering Skeletal Muscle Tissues from Murine Myoblast Progenitor Cells and Application of Electrical Stimulation

Conditions that promote primary human skeletal myoblast culture and muscle differentiation in vitro

Hydrogel biomaterials and their therapeutic potential for muscle injuries and muscular dystrophies

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