Effects of a collagen matrix containing basic fibroblast growth factor on wound contraction

Abstract

We evaluated the effectiveness of basic fibroblast growth factor (bFGF) in inhibiting wound contraction, both alone and in combination with collagen matrix, using a simulated in vivo delayed healing type model. We also studied the mechanisms involved in this inhibition in in vitro experiments using fibroblast populated collagen gels. As a result, we were able to demonstrate that both collagen matrix and bFGF significantly inhibited wound contraction; especially, bFGF acted in a dose-dependent fashion. Interestingly, their combination was much more effective than either collagen matrix or bFGF alone, a finding that was supported by the histopathological data. Wounds treated with collagen matrix, but not control wounds, showed horizontal rearrangement of collagen fibers in dermis as well as evidence of fibroblast proliferation, which was not observed in scar regions surrounded by normal dermis. Using fibroblast-populated collagen gel contraction as an in vitro model, we found that bFGF significantly inhibited contraction. Taking all these results together, it was concluded that collagen matrix is useful not only as a carrier of cytokines such as bFGF, but also for the quick closure of chronic wounds, thereby preventing contracture, which remains one of the most challenging problems in treating this type of wound. Application of bFGF-treated collagen matrix to chronic wounds such as decubitus, and diabetic and leg ulcers may prove to be highly beneficial in clinical practice.

Effect of Porcine Small Intestinal Submucosa on Acute Full‐Thickness Wounds in Dogs

“…There are numerous studies that report inhibition of wound contraction after implanting a collagenous ECM into full-thickness wounds. 6,[77][78][79][80][81] This effect is used, and indeed desired, in human wound treatment, where contraction is usually unwanted. In veterinary applications, however, contraction is often a welcome and important attribute of open wound management, unless the wounds are over flexor and extensor aspects of joints, and adjacent to orifices.…”

Cell colonization in degradable 3D porous matrices

“…Thus, one of the approaches to minimize scarring is to increase the tensile strength via wound dressing materials. 3D collagen sponges have been used alone 145,146 or in conjunction with basic fibroblast growth factor, 147 fibronectin or hyaluronic acid. 148 Exogenous collagen increased wound tensile strength and increased degree of reepithelialization i.e., early dermal and epidermal wound healing.…”

Derivation of Functional Smooth Muscle Cells from Multipotent Human Hair Follicle Mesenchymal Stem Cells

“…18,19 In addition, application of bFGFcontaining collagen sponges in full-thickness wounds reduced aSMA expression and wound contraction in a rat model. 29,30 In agreement, we showed that treatment of HFCs with bFGF decreased aSMA fibers significantly and diminished the ability of these cells to contract fibrin. Interestingly, the effect of bFGF on P-aSMA was less dramatic.…”