Effects of a blend of essential oils on some end products of in vitro rumen fermentation

Spanghero, M.; Zanfi, C.; Fabbro, Elena; Scicutella, N.; Camellini, C.

doi:10.1016/j.anifeedsci.2007.05.048

Cited by 53 publications

(41 citation statements)

References 17 publications

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“…The author 46 further suggested that at pH 6-7 the total VFA concentration decreased and A: P ratio increased. A similar reduction in acetate proportion,A:P ratio and increased TVFA at lower pH (5-5.5) has been noted by earelier workers 48 . Decrease in TVFA with inclusion of essential oil without any change in pH has been found 49 .…”

Section: Total Volatile Fatty Acid (Tvfa) Concentration and Acetate: supporting

confidence: 50%

An in vitro trial to study the effect of inclusion of different levels of coconut oil on sheep rumen methanogenesis and fermentation processes

Mukharji¹,

Srivastava²,

Srivastava³

2014

PBJ

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An in vitro trial was conducted in fermentation vessels under anaerobic conditions using composite feed-1 and composite feed-2 with inclusion of different levels of Coconut Oil (CO) along with buffered rumen liquor of sheep to observe the effects of oil on rumen methanogenesis and fermentation. The Composite Feed-1(CF-1) consisted of 40% of concentrate and Composite Feed-2 (CF-2) had 25 % of concentrate. The different levels of CO used were 0.5,1 and 2 µl/ml. Among the gas production and methane concentration parameters the inclusion of CO showed a significant decrease of 38.67%. A non significant effect on gas production was noted with the inclusion of CO in CF-2. Further, a decrease in total gas production and methane concentration was observed which was highly pronounced in CF-1 as compared to CF-2. The rumen digestibility by inclusion of CO in CF-1 and CF-2 decreased significantly (P < 0.01) from control.The inclusion of CO in CF-2 did not affect Ammonia Nitrogen (NH3 -N) concentration, while in CF-1 the CO inclusion increased the NH3 -N concentration. The inclusion of CO decreased the Total volatile fatty acid (TVFA) concentration significantly (P < 0.01). In both the diets namely CF-1 and CF-2 the A:P ratio was unaffected by CO inclusion. The inclusion of CO in CF-1 had a non significant effect in pH. However, the inclusion of CO in CF-1 and CF-2 decreased the protozoa number significantly (P < 0.01). Inclusion of oil tended to decrease Gas production (GP), Metabolizable energy (ME), Short Chain Fatty Acid (SCFA), True Degradability of Dry Matter (TDDM) and Microbial Biomass Production (MBP). The effect was more pronounced when CO level was 1.0µl/ml or higher. From the above gas production and fermentation parameters noticed in two diets CF-1 and CF-2 with inclusion of different levels of CO, it can be envisaged that increasing the proportion of concentrate in diets and using CO as a feed additive increases the rumen efficiency by reducing the methane concentration and gas production.

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Section: Total Volatile Fatty Acid (Tvfa) Concentration and Acetate: supporting

confidence: 50%

An in vitro trial to study the effect of inclusion of different levels of coconut oil on sheep rumen methanogenesis and fermentation processes

Mukharji¹,

Srivastava²,

Srivastava³

2014

PBJ

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“…The inhibitory activity of functional oils in relation to the bacteria population involve ruminal ammonia production, which was found in vitro by McIntosh et al (2003) and Newbold et al (2004), but in this research, the inclusion of tCNSL did not influence the concentration of NH 3 -N concentration in rumen (table IV), in agreement with results in vitro (Spanghero et al, 2008) and in vivo (Khiaosa-Ard and Zebeli, 2013). Castillejos et al (2007) suggest that a long period of adaptation of rumen bacteria to functional oils (at least 4 weeks) may be required to achieve the depression in the concentration of NH 3 -N concentrations, which was not achieved in this experiment.…”

Section: Resultssupporting

confidence: 81%

“…Spanghero et al (2008) suggest the effects of functional oils may be pH dependent, acting more effectively when rumen pH is close to 5.5 which were not observed in the present study. Thus, this mechanism dependence on a more acidic ruminal pH to express the maximum effect of some plant extracts, can also affect the responses recorded for the use of tCNSL (table IV).…”

Section: Resultscontrasting

confidence: 48%

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Aumento das doses dietéticas de líquido de casca de castanha de caju na digestibilidade ruminal e intestinal de nutrientes em novilhos alimentados com uma dieta rica em grãos

et al. 2017

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sUMMAryThe objective was to evaluate the inclusion of technical cashew nut shell liquid (tCNSL) in the concentrate for cattle fed on a high-grain diet on feed intake, ruminal and total apparent digestibility coefficients of nutrients, fermentation characteristics and microbial efficiency. Four Holstein steers (445 ± 23.39 kg of body weight) fitted with ruminal and duodenal cannulae were used. The diets had (in DM basis) 144 g kg-1 of crude protein and 799 g kg-1 of total digestible nutrients, with a forage to concentrate ratio of 15:85. The experimental design was a 4 x 4 Latin square with 10-day experimental periods. The diets consisted of 0; 300; 600 and 1200 mg of tCNSL in DM of the concentrate. Intake and total apparent digestibility coefficient of nutrients, on DM basis, were not affected by experimental diets. However, ruminal digestibility of ether extract showed quadratic response in which animals fed 0 and 300 mg tCNSL kg-1 of concentrate had the lowest values for ruminal digestibility. Ruminal ammonia nitrogen, plasma urea nitrogen and microbial efficiency were not affected by treatments. However, ruminal pH tended to increase when animals were fed with tCNSL. Objetivou-se avaliar os efeitos da inclusão do líquido da casca da castanha de caju (tCNSL) na dieta de bovinos alimentados com dietas ricas em grão, sobre a ingestão e o coeficiente de digestibilidade ruminal e total dos nutrientes; as características de fermentação ruminal, eficiência de síntese microbiana no rúmen e parâmetro sanguíneo, através do nitrogênio ureico plasmático. Foram utilizados quatro novilhos da raça Holandesa (445 ± 23,39 kg de peso vivo) providos de cânula ruminal e duodenal, recebendo uma dieta com 144 g kg-1 de proteína bruta e 799 g kg-1 de nutrientes digestíveis totais, com uma relação volumoso:concentrado de 15:85. O delineamento experimental foi o quadrado latino 4 x 4 com 10 dias de períodos experimentais. As dietas foram compostas de 0, 300, 600 e 1200 mg de tCNSL na matéria seca (MS) do concentrado. A ingestão e os coeficientes de digestibilidade aparente total dos nutrientes, com base na MS, não foram influenciados pela inclusão de tCNSL na dieta. O coeficiente de digestibilidade ruminal (CDR) do extrato etéreo apresentou resposta quadrática, em que os animais que consumiram 0 e 300 mg tCNSL d-1 apresentaram menores valores de CDR. O uso do LCCt não influenciou o nitrogênio amoniacal no rúmen, o nitrogênio ureico plasmático e a eficiência de síntese microbiana. No entanto, houve tendência de maior pH ruminal quando os animais consumiram o LCCt. PAlAvrAs-chAve AdicionAisAditivo. Cardol. Cardanol. Digestibilidade. Fermentação ruminal.

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“…In our previous studies, it was observed that a combination of four EO active components at different ratios had a varied potential of reducing ruminal methane production (Lin et al, 2011). It is still unclear, however, what the effect on rumen fermentation and methane production will be when the combined natural essential oils are added, though there are reports that combining essential oils (CEO) may enhance their ability to reduce rumen methane and ammonia production (Newbold et al, 2004;Spanghero et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

The effects of combined essential oils along with fumarate on rumen fermentation and methane production <i>in vitro</i>

Lin¹,

Lü²,

Wang³

et al. 2012

J. Anim. Feed Sci.

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Two trials were conducted to investigate the effect of a combination of essential oils (CEO) along with fumarate on in vitro rumen fermentation. In trial 1, the essential oil (EO) from thyme, oregano, cinnamon and lemon were mixed at five different ratios. The CEO were applied at levels of 0-500 mg/l. Addition of CEO decreased gas, methane, total volatile fatty acid (VFA) production at 24 h incubation in a dose-dependent manner. Methane tended to decrease much more than total VFA and gas at the same EO level. The mixture of oils at an equal ratio at 500 mg/l that decreased methane much more than VFA was chosen as the optimal combination. In trial 2, the optimal combination was used with 0, 5, 10 or 15 mmol/l of monosodium fumarate. Addition of fumarate further decreased methane production, with 10 mmol/l fumarate resulting in the largest reduction (80.2%) and the smallest decrease in total VFA (5.7%) and gas production (16.7%). Quantification of several ruminal microbe populations by RT-PCR showed that the optimal combination sharply decreased ruminal protozoa; the populations of fungi and fibrolytic bacteria were also decreased. In summary, at an appropriate level, CEO can inhibit methane production. Inclusion of fumarate can further decrease it, which is attributed mainly to inhibition of protozoa and methanogens.

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Effects of a blend of essential oils on some end products of in vitro rumen fermentation

Cited by 53 publications

References 17 publications

An in vitro trial to study the effect of inclusion of different levels of coconut oil on sheep rumen methanogenesis and fermentation processes

An in vitro trial to study the effect of inclusion of different levels of coconut oil on sheep rumen methanogenesis and fermentation processes

Aumento das doses dietéticas de líquido de casca de castanha de caju na digestibilidade ruminal e intestinal de nutrientes em novilhos alimentados com uma dieta rica em grãos

The effects of combined essential oils along with fumarate on rumen fermentation and methane production <i>in vitro</i>

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