Effects of a Bacteria-Based Probiotic on Ruminal pH, Volatile Fatty Acids and Bacterial Flora of Holstein Calves

Qadis, Abdul Qadir; Goya, Satoru; Ikuta, Kentaro; Yatsu, Minoru; Kimura, Atsushi; Nakanishi, Shusuke; Satō, Shigeru

doi:10.1292/jvms.14-0028

Cited by 54 publications

(53 citation statements)

References 38 publications

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“…Numerous studies have reported beneficial effects of probiotics on the health status and performance of calves (Mudgal and Baghel 2010;Bayatkouhsar et al 2013;Qadis et al 2014;Soto et al 2014). Most of the experiments showed increased weight doi: 10.17221/8727-CJAS gain and improved feed conversion ratios, but few studies also monitored a long-term survival of the probiotics administered.…”

Section: Discussionmentioning

confidence: 99%

Persistence of bifidobacteria in the intestines of calves after administration in freeze-dried form or in fermented milk

Geigerová¹,

Vlková²,

Bunešová³

et al. 2016

CZECH J ANIM SCI

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ABSTRACT:In order to improve the gut microbiome of calves, probiotic bacteria can be fed as active livingcells (fermented milk), or as live but inactive (freeze-dried) cultures. Ten bifidobacterial strains with suitable probiotic properties (as determined in our previous study) were tested for survival during the freeze-drying process, and screened for their ability to ferment cow's milk. The viability of both freeze-dried and live-cell cultures during storage was also tested. All of the strains tested were able to ferment cow's milk, with average counts of 8.26 ± 0.62 log CFU/ml. Eight out of the ten strains were able to survive in milk for 2 months in counts higher than 10 6 CFU/ml. Bifidobacteria showed high viability following the freeze-drying process, with average numbers of 9.03 ± 0.22 log CFU/vial and did not decrease after 12 months of storage. The mixture of rifampicin-resistant variants of bifidobacteria (RRBs) was fed to 2-day-old dairy Charolais calves in the form of living-cells, or as freeze-dried bacteria. The control group was given no probiotics. Survival of the RRBs administered and the numbers of other bacterial groups in faecal samples was monitored by culturing. Bifidobacteria that were administered passed successfully through the upper parts of the gastrointestinal tract, and were found in numbers higher than 10 9 CFU/g for two weeks. RRBs colonized the intestines of calves for at least 63 days in both treatment groups. Significantly higher total counts of bifidobacteria were found in the treated groups, compared to the control group. Reduction in Escherichia coli and total coliforms numbers, and an increase in lactobacilli counts were observed in both experimental groups following the application of the probiotic mixtures. Our results show that both forms of administering probiotic bifidobacteria to calves are effective, but that the freeze-dried form is more suitable from a practical viewpoint.

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Section: Discussionmentioning

confidence: 99%

Persistence of bifidobacteria in the intestines of calves after administration in freeze-dried form or in fermented milk

Geigerová¹,

Vlková²,

Bunešová³

et al. 2016

CZECH J ANIM SCI

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“…Due to the strongly acidic and proteolytic conditions in animal stomachs [Bilik et al, 2014;Dono et al, 2014;Moesseler et al, 2010Moesseler et al, , 2012Mohammed et al, 2014;Olukosi and Dono 2014;Qadis et al, 2014], the stability and activity of phytase under gastric conditions can be critical to its catalytic efficacy. However, only several phytases reported to date were characterized as stable under gastric or acidic conditions Fugthong et al, 2010;Garrett et al, 2004;Huang et al, 2008;Singh and Satyanarayana, 2009].…”

Section: Introductionmentioning

confidence: 99%

A Novel Phytase Derived from an Acidic Peat-Soil Microbiome Showing High Stability under Acidic Plus Pepsin Conditions

Tan¹,

Wu²,

Xie³

et al. 2016

Microb Physiol

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Four novel phytases of the histidine acid phosphatase family were identified in two publicly available metagenomic datasets of an acidic peat-soil microbiome in northeastern Bavaria, Germany. These enzymes have low similarity to all the reported phytases. They were overexpressed in Escherichia coli and purified. Catalytic efficacy in simulated gastric fluid was measured and compared among the four candidates. The phytase named rPhyPt4 was selected for its high activity. It is the first phytase identified from unculturable Acidobacteria. The phytase showed a longer half-life than all the gastric-stable phytases that have been reported to date, suggesting a strong resistance to low pH and pepsin. A wide pH profile was observed between pH 1.5 and 5.0. At the optimum pH (2.5) the activity was 2,790 μmol/min/mg at the physiological temperature of 37°C and 3,989 μmol/min/mg at the optimum temperature of 60°C. Due to the competent activity level as well as the high gastric stability, the phytase could be a potential candidate for practical use in livestock and poultry feeding

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“…plantarum , E . faecium and Clostridium butyricum on the ruminal components of weaned calves [20]. In this study, we examined the effects of different doses of a multi-strain BP on ruminal pH and VFA, lactic acid, ammonia nitrogen (NH 3 -N) and plasma metabolite concentrations in cattle with SARA.…”

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confidence: 99%

Effects of a bacterial probiotic on ruminal pH and volatile fatty acids during subacute ruminal acidosis (SARA) in cattle

Goto

Qadis

Kim

et al. 2016

The Journal of Veterinary Medical Science

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Effects of a bacterial probiotic (BP) on ruminal fermentation and plasma metabolites were evaluated in four Holstein cattle (body weight, 645 ± 62 kg; mean ± SD) with induced subacute ruminal acidosis (SARA). SARA was induced by feeding a SARA-inducing diet, and thereafter, 20, 50 or 100 g per head of a commercial BP was administered for 7 consecutive days during the morning feeding. Cattle without BP served as the control. The 24-hr mean ruminal pH in the control was lower, whereas those in the BP groups administered 20 or 50 g were significantly higher compared to the control from days 2 to 7. Circadian patterns of the 1-hr mean ruminal pH were identical (6.4–6.8) among all cattle receiving BP. Although the mean minimum pH in the control on day –7 and day 0 was <5.8, the pH in the treatment groups on day 7 was >5.8 and significantly higher than that of the control group ( >5.2). Ruminal volatile fatty acid (VFA) concentrations were not affected by BP treatment; however, the BP groups had lower lactic acid levels compared with the control group at 20:00 on day 7. Additionally, non-esterified fatty acid levels decreased from 8:00 to 20:00 in all BP groups on day 7. These results suggest that administration of 20 to 50 g of a multi-strain BP for 7 days might improve the low pH and high lactic acid level of the ruminal fluid in SARA cattle.

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Effects of a Bacteria-Based Probiotic on Ruminal pH, Volatile Fatty Acids and Bacterial Flora of Holstein Calves

Cited by 54 publications

References 38 publications

Persistence of bifidobacteria in the intestines of calves after administration in freeze-dried form or in fermented milk

Persistence of bifidobacteria in the intestines of calves after administration in freeze-dried form or in fermented milk

A Novel Phytase Derived from an Acidic Peat-Soil Microbiome Showing High Stability under Acidic Plus Pepsin Conditions

Effects of a bacterial probiotic on ruminal pH and volatile fatty acids during subacute ruminal acidosis (SARA) in cattle

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