Effects of a 980-Nm Diode Laser’s Activation of 2.5% Naocl and 2% Chlorhexidine Antifungal Irrigation Solutions on Candida Albicans Biofilms

Iselinni, Chitra; Meidyawati, Ratna; Djauharie, RA Nilakusuma

doi:10.22159/ijap.2017.v9s2.04

Cited by 5 publications

(3 citation statements)

References 11 publications

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“…Previous literature has not found an explanation of how the diode laser of a wavelength of 980 nm has affected the biofilm of C. albicans. Several researches have examined how bacteria are affected by lasers, the results suggest to be applied to C. albicans [ 27 ].…”

Section: Discussionmentioning

confidence: 99%

Different wavelengths of laser: are they significant for treatment of denture stomatitis?: an in-vitro study

El-Din,

El-Sharkawy,

Abdelrahman

et al. 2024

BMC Oral Health

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Background Denture stomatitis (DS) is an inflammatory disorder that affects the mucosal surface underneath the dentures and frequently causes oral mucosal irritation, discomfort, and altered taste perception, which prevents people from consuming enough nutrients. One of the main causes of DS is an overgrowth of the fungus Candida albicans (C. albicans). A possible alternative treatment for Candida infections is thought to be laser therapy. The aim of this study was to evaluate how different wavelengths of laser would affect growth and pathogenic properties of Candida albicans. Methods A concentration of 106 viable cells/ml of Candida albicans were used in the preparation process. Four groups were created from the specimens. Culturing of the control group was completed with no intervention. The other 3 groups received laser radiation for 60 seconds at a power of 1W. The 2nd and 3rd groups were irradiated with diode laser at a wavelength of 940 nm and 980 nm respectively. The 4th group was irradiated with Nd-YAG laser at a wavelength of 1064 nm. Turbidimetric growth was defined as variations in the optical density of fungal growth. These measures were made at three different times: baseline, 48 hours, and 72 hours. Results In both groups of diode laser, the growth of Candida albicans showed no remarkable differences at baseline, after 48 and 72 hours using a power of 1 W and duration of 60 seconds. The Nd-YAG group showed significant increase in optical density after 48 hrs then significant decrease after 72 hrs. The optical density values in the control group showed no notable difference between the control and diode study groups at different time periods. However, the Nd:YAG group showed a statistically significant difference compared to the control and the 2 diode laser groups. Conclusions Different laser parameters have a different effect on growth and pathogenic properties of Candida albicans. Diode laser therapy with wavelengths 940 and 980 nm used in continuous mode with power of 1 W for duration of 60 seconds can result in proliferation of Candida albicans instead of destroying them. Nd:YAG laser, used in pulsed mode, with power of 1 W for a duration of 60 seconds can be used to destroy Candida albicans and therefore, can be used as an effective treatment for denture stomatitis.

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Section: Discussionmentioning

confidence: 99%

Different wavelengths of laser: are they significant for treatment of denture stomatitis?: an in-vitro study

El-Din,

El-Sharkawy,

Abdelrahman

et al. 2024

BMC Oral Health

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“…Nevertheless, diode Laser activation of EDTA enhances removing the smear layer from the apical third of the root canal [52]. Moreover, diode laser activation of CHX increased its penetration through dentinal tubes [53]. The previous enhancement in irrigants features, which increased in disinfecting and cleaning of the root canal system rather than the healing characters of the diode laser itself [54], may re ect in pain decreasing in patients of diode laser as an IAS group in comparison with the control group through all follow-up periods.…”

Section: Discussionmentioning

confidence: 99%

Can Diode Laser 810 decrease Post Endodontic Pain in Patients with Asymptomatic Necrotic Maxillary Incisors? A Four-Arm Randomized Controlled Trial

Abbara,

Tolibah,

Akil

et al. 2023

Preprint

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Background and aim: In light of the development of modern technologies and the insertion of laser in the context of endodontic treatment, this study aimed to find the best method for applying the 810 diode laser to relieve post-endodontic pain on necrotic maxillary incisors with periapical lesions within a single-visit treatment. Methods: Eighty patients with a necrotic incisor, diagnosed with asymptomatic periodontitis, received standardized cleaning and shaping procedures, then divided equally into four groups: G1: control group with no laser application, G2: applying the diode laser as an irrigation activation system (IAS), G3: applying the diode laser from the buccal and palatal mucosa, G4: applying the diode laser as an IAS and from buccal and palatal mucosa. The postoperative pain (PP) was assessed using the visual analog scale (VAS) 1, 3, 7, and 14 days after the treatment. The mean values of the VAS score were statistically analyzed. Results: During 14 days after treatment, there was a statistically significant difference between mean values of VAS scores in the four groups (P value < 0.05); G1 scored the highest score, whereas G4 showed the lowest one. Moreover, G4 showed favorable outcomes compared with G2 and G3 during the first three days after treatment. Conclusion: Diode laser reduced the PP after necrotic teeth with large-sized apical lesion treatment, whereas using diode laser either as an IAS or LLLT reduced the PP compared with the control group. Moreover, the usage of a diode laser in both previous techniques represents the best protocol for PP relieve during 14 days of treatment.

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“…Among other techniques, laser based methods have been studied as relevant techniques for bacterial management with a potential in wound treatment applications. [1,2]. A comparison of the cell lysis method mentioned above is given in several review articles [3][4][5][6].…”

Section: Introductionmentioning

confidence: 99%

Application of Laser-Induced Breakdown Cavitation Bubbles for Cell Lysis in Vitro

2019

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Objective: Understanding the basic mechanism of the cavitation bubble action on living cells as a crucial step of development and application ofsophisticated methods based on controlled cavitation in cell behaviour manipulation. Optimisation of parameters in order to expand cell lysis regioncreated by a single bubble.Methods: The cavitation bubbles are generated by the laser-induced breakdown method. The impact of controlled cavitation bubble on thebiological system is synchronously monitored under a microscope and recorded. Visualization of the cavitation bubble course is monitored by a highspeedcamera. The impact of technology on the healthy confluent cell layer is verified. Evaluation of the cavitation bubbles´ effect on cells in real timeand by subsequent analysis of the cell lysis region and impact of the cavitation bubble on cell viability is carried out by optical visualization and life/dead fluorescence staining.Results: Cavitation bubble induced in distance of 1.5 mm from the cell surface overcomes properties of sessile bubble and enables to create cell lysisregion over 1000 μm in diameter due to transient shear stress produced by liquid displaced by the bubble expansion.Conclusion: Cell lysis region is strongly dependent on the spot laser energy (SLE) and the bubble induction distance from cells. This knowledge iscrucial for application in chemical free cell lysis in vitro, wound induction for experimental purposes and cell layers patterning in desired scale.

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Effects of a 980-Nm Diode Laser’s Activation of 2.5% Naocl and 2% Chlorhexidine Antifungal Irrigation Solutions on Candida Albicans Biofilms

Cited by 5 publications

References 11 publications

Different wavelengths of laser: are they significant for treatment of denture stomatitis?: an in-vitro study

Different wavelengths of laser: are they significant for treatment of denture stomatitis?: an in-vitro study

Can Diode Laser 810 decrease Post Endodontic Pain in Patients with Asymptomatic Necrotic Maxillary Incisors? A Four-Arm Randomized Controlled Trial

Application of Laser-Induced Breakdown Cavitation Bubbles for Cell Lysis in Vitro

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