Effects of 50Hz electromagnetic fields on voltage-gated Ca2+ channels and their role in modulation of neuroendocrine cell proliferation and death

Grassi, Claudio; D’Ascenzo, Marcello; Torsello, Angela; Martinotti, Giovanni; Wolf, Federica I.; Cittadini, Achille; Azzena, Gian Battista

doi:10.1016/j.ceca.2003.09.001

Cited by 190 publications

(131 citation statements)

References 38 publications

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“…Our FCM results showed that the cells treated by paclitaxel were blocked at G2/M phase (Figure 3), and this phenomenon also highlighted the especial effects of paclitaxel. Furthermore, DNA can be damaged in a variety of ways, for example, most antitumor drugs or an external source of ionizing radiation can cause DNA damage (Grassi et al 2004;Wolf et al 2005). Our results of comet assay showed that DNA damage was increased after the cells were treated with SMF and/or paclitaxel.…”

Section: Discussionmentioning

confidence: 48%

Biologic effects of SMF and paclitaxel on K562 human leukemia cells

Sun

Chen²,

Qi³

et al. 2012

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Abstract. In this study, we evaluated the ability of 8.8 mT static magnetic fields (SMF) to enhance the in vitro action of a chemotherapeutic agent, paclitaxel, against K562 human leukemia cells. We analyzed the cell proliferation, cell cycle distribution, DNA damage and alteration of cell surface and cell organelle ultrastructure after K562 cells were exposed to paclitaxel in the presence or absence of 8.8 mT SMF. The results showed that in the presence of SMF, the efficient concentration of paclitaxel on K562 cells was decreased from 50 to 10 ng/ml. Cell cycle analysis indicated that K562 cells treated with SMF plus paclitaxel were arrested at the G2 phase, which was mainly induced by paclitaxel. Through comet assay, we found that the cell cycle arrest effect of paclitaxel with or without SMF on K562 cells was correlated with DNA damage. The results of atomic force microscopy and transmission electron microscopy observation showed that the cell ultrastructure was altered in the group treated with the combination of SMF and paclitaxel, holes and protuberances were observed, and vacuoles in cytoplasm were augmented. Our data indicated that the potency of the combination of SMF and paclitaxel was greater than that of SMF or paclitaxel alone on K562 cells, and these effects were correlated with DNA damage induced by SMF and paclitaxel. Therefore, the alteration of cell membrane permeability may be one important mechanism underlying the effects of SMF and paclitaxel on K562 cells.

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Section: Discussionmentioning

confidence: 48%

Biologic effects of SMF and paclitaxel on K562 human leukemia cells

Sun

Chen²,

Qi³

et al. 2012

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“…Cell systems, like mammalian cells, possess the potential for EMF interaction. For example, the depolarization of a neuron produces an influx of Ca2+ ions through the membrane, which is related to the intensity of a magnetic field (Grassi et al, 2004;Pall, 2013). If a physiologicallypatterned, intensity-adjusted EMF is applied to the membrane with the exact resonant frequency of Ca2+ gated channels, then this EMF application can activate or stimulate the particular cell.…”

Section: Introductionmentioning

confidence: 99%

Phonon Guided Biology. Architecture of Life and Conscious Perception Are Mediated by Toroidal Coupling of Phonon, Photon and Electron Information Fluxes at Discrete Eigenfrequencies.

Meijer

Geesink

2016

Neuroquantology

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Recently, a novel biological principle, revealing specific electromagnetic (EM) radiation frequencies that sustain life, was presented by us on the basis of an evaluation of 175 biological articles concerning beneficial effects of electromagnetic waves on the state of living cells. This concept was also based on a very similar range of frequencies emitted by a clay-mineral catalyst of RNA synthesis that may have been instrumental in the evolutionary initiation of first life, and therefore was tentatively designated as "Algorithm of Life". The particular spectrum of frequency bands indicate that nature seems to employ discrete eigenfrequencies or standing waves that match precisely with an acoustic scale, with frequency ratios of 1:2, and closely approximated by 2:3, 3:4, 3:5, 4:5 and higher partials, allowing the discrete frequencies to be expressed in scalars. Our further studies clearly indicate now that this "life algorithm" pattern matches very well with the mathematical calculations of W. Ritz (1909) to compute eigenfrequencies of the sound induced geometric patterns. These have been earlier demonstrated through membrane vibration experiments of E. Chladni (1787), as well as several follow up studies from 1970-2013. Our findings, therefore, touch upon the science of acoustics, also since we show that the discrete frequencies could be modeled by music torus geometry. We postulate that the spectrum of EM frequencies detected, exhibit a quantum ordering effect on life cells on the basis of induction of geometric wave patterns. These constitute phonon/photon and electron wave energies, and quantum oscillations at far-infrared frequencies, that are communicated through toroidal constructive interference into scalar wave information. This idea is supported through our identification of potential intrinsic toroidal eigenfrequencies and minimal energy levels. The particular torus topology for information processing may also provide quantum error correction and protection against decoherence. Finally, we propose a phonon guided organization of cells and integral brain function by three elementary processes: 1) A phonon mediated geometric organization of coherent arrangement of water molecules in cellular plasma, leading to instructive functional organization of cellular structures and metabolic processes and enabling the origination and sustainment of life processes. 2) Toroidal phonon/photon/electron coupling, protecting standing wave coherency of resonant cell components such as proteins and DNA. 3) A toroidal integration of electromagnetic and phononic fluxes of information into scalar standing waves, promoting quantum flux of informational excitons such as Ca2+-ions and electrons (polaron and polariton formation). Our brain, therefore, can be placed in a 4+1 geometry, supported by internal and external quantum states and makes use of geometrical defined information fluxes, that are converted to standing waves. The integration of these interrelated processes is considered to be instrumental in the creatio...

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“…The experiment of Grassi, et al [15] showed that the Ca 2+ current through a calcium channel is almost unaffected by electromagnetic fields. And the enhancement of Ca 2+ current density was due to the increased number of voltage-gated Ca 2+ channels.…”

Section: The Modelmentioning

confidence: 99%

Noise induced calcium oscillations in a cell exposed to electromagnetic fields

Zhang

Chen

Yuan

et al. 2015

BME

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Abstract. The effects of noise on the calcium oscillations in a cell exposed to electromagnetic fields are described by a dynamic model. Noise is a very important factor to be considered in the dynamic research on the calcium oscillations in a cell exposed to electromagnetic fields. Some meaningful results have been obtained here based on the discussion. The results show that the pattern of intracellular calcium oscillations exposure to electromagnetic fields can be influenced by noise. Furthermore, the intracellular calcium oscillations exposure to electromagnetic fields can also be induced by noise. And the work has also studied the relationships between the voltage sensitive calcium channel's open probability and electromagnetic field. The result can provide new insights into constructive roles and potential applications of selecting appropriate electromagnetic field frequency during the research of biological effect of electromagnetic field.

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Effects of 50Hz electromagnetic fields on voltage-gated Ca2+ channels and their role in modulation of neuroendocrine cell proliferation and death

Cited by 190 publications

References 38 publications

Biologic effects of SMF and paclitaxel on K562 human leukemia cells

Biologic effects of SMF and paclitaxel on K562 human leukemia cells

Phonon Guided Biology. Architecture of Life and Conscious Perception Are Mediated by Toroidal Coupling of Phonon, Photon and Electron Information Fluxes at Discrete Eigenfrequencies.

Noise induced calcium oscillations in a cell exposed to electromagnetic fields

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