EFFECTS OF 5,6‐DIHYDROXYTRYPTAMINE AND 5,7‐DIHYDROXYTRYPTAMINE ON THE RAT CENTRAL NERVOUS SYSTEM AFTER INTRAVENTRICULAR OR INTRACEREBRAL APPLICATION AND ON BLOOD PLATELETS <i>IN VITRO</i>

Prada, M. Da; Pieri, L.; Keller, H. H.; Pieri, Margherita; Bonetti, E. P.

doi:10.1111/j.1749-6632.1978.tb31551.x

Cited by 27 publications

(9 citation statements)

References 43 publications

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“…Conversely, drugs that reduce 5-HT neurotransmission by antagonizing postsynaptic 5-HT receptors or acting as agonists at 5-HT 1A autoreceptors facilitate sex behavior (Ahlenius, Larsson, & Arvidsson, 1989;Da Prada, Bonetti, Scherschlicht, & Bondiolotti, 1985;Fernandez-Guasti & Escalante, 1991). Furthermore, the administration of 5,7-dihydroxytryptamine or p-chloroamphetamine (drugs that selectively destroy 5-HT nerve terminals) also stimulates sexual behavior (Da Prada, Pieri, Keller, Pieri, & Bonetti, 1978;Fernandez-Guasti & Escalante, 1991;Sodersten, Berge, & Hole, 1978). However, it has been noted that an extensive lesion is necessary to achieve this behavioral effect (Sodersten et al, 1978).…”

Section: Discussionmentioning

confidence: 99%

Treatment with a serotonin-depleting regimen of MDMA prevents conditioned place preference to sex in male rats.

Straiko¹,

Gudelsky²,

Coolen³

2007

Behavioral Neuroscience

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Among young adults, 3,4-methylenedioxymethamphetamine (MDMA, "ecstasy") is a popular drug of abuse, and anecdotal evidence indicates that repeated use of MDMA may result in impairments in sexual function and decreased sex drive in human users. There has been little investigation of the effects of MDMA on sexual function in rodents. In the present study, the authors determined that in male rats (Rattus novegicus) tested in a sexually naïve or a sexually experienced state, administration of a serotonin (5-HT)-depleting regimen of MDMA did not produce a change in mount, intromission, and ejaculation latency or in mount and intromission frequency compared with such latency and frequency in vehicle-treated control rats. In contrast to vehicle-treated rats, MDMA-treated rats did not form a conditioned place preference (CPP) to sex. Failure of MDMA-treated rats to form CPP to sex may be due to MDMA-induced impairments in circuits mediating sexual reward.

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Section: Discussionmentioning

confidence: 99%

Treatment with a serotonin-depleting regimen of MDMA prevents conditioned place preference to sex in male rats.

Straiko¹,

Gudelsky²,

Coolen³

2007

Behavioral Neuroscience

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“…Albumin binding capacity was not affected at ymolar concentrations; however, a higher concentration (10 y~) inhibited the binding of serotonin to albumin (not shown). Other drugs, known to deplete serotonin from platelets, such as the indole neurotoxins (Da Prada et al, 1978) and chlorpromazine (Laubscher and Pletscher, 1979), showed no discrimination between the two proteins and inhibited the binding of serotonin to brain and platelet proteins to the same extent. Dopamine, a component of platelet granules and a potent inhibitor of the binding of serotonin to SBP, was an order of magnitude less effective in inhibiting the binding of serotonin to platelet proteins.…”

Section: Effect Of Inhibitors Of Serotonin Binding To Proteins Isolatmentioning

confidence: 98%

“…35, (1980. Numerous investigators use blood platelets as a model for serotonergic neurons (Pletscher, 1968;Sneddon, 1973;Stahl, 1977;Da Prada et al, 1978). The model is thought to be especially valuable for the study of brain disease in humans because of the availability of platelets.…”

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confidence: 99%

Differences Between Intracellular Platelet and Brain Proteins that Bind Serotonin

Tamir

Bebirian

Casper

1980

Journal of Neurochemistry

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Two proteins with binding capacity highly specific for serotonin are present in the 100,000 g supernatant obtained from rat platelets: a glycoprotein and albumin. They were purified by means of (NH4)2SO4 fractionation, Sephadex sieve chromatography, and affinity column chromatography. The two proteins differed in most of their physical and chemical properties: (a) migration on 7.5% acrylamide gel of the complex (protein-Fe+2-[3H]serotonin); (b) molecular weight (sodium dodecyl sulfate gels); (c) carbohydrate reaction; (d) binding capacity and binding constants; (e) effect of reserpine; (f) heat stability; and (g) isoelectric point. However, they showed two similar properties: sensitivity to trypsin and dependence on Fe+2 for serotonin binding. The properties of both the glycoprotein and albumin differ considerably from those of serotonin binding protein of brain (SBP), which was not detected in platelets. Since brain serotonin binding protein is thought to be involved in storage of the amine, these results suggest that the storage form of serotonin in rat platelets is different from that in rat brain. These results also imply that the storage of serotonin in platelets may not serve as a model for the storage of serotonin in nerve terminals of the brain.

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“…Results from different laboratories have shown that blood platelets of rats with SPD have a low content of 5-HT and adenosine 5'-triphosphate (ATP) (Holmsen & Weiss, 1970;Tschopp & Weiss, 1975) and that these abnormal platelets when seen with the electron microscope appear to contain only a few atypical 5-HT organelles (Da Prada, Pieri, Keller, Pieri & Bonetti, 1978;Da Prada, Richards & Lorez, 1978). In addition, previous data from our laboratory have shown that platelets from humans with SPD (Lorez, Richards, Da Prada, Picotti, Pareti, Capitanio & Mannucci, 1978) and from Fawn-hooded rats have a low storage capacity not only for 5-HT but also for mepacrine, a fluorescent probe which selectively accumulates in the 5-HT organelles (Lorez, Da Prada, Rendu & Pletscher, 1977).…”

Section: Catecholamines and 5-hydroxytryptamine In Spd Rat Plateletsmentioning

confidence: 99%

Content and Subcellular Localization of Catecholamines and 5‐hydroxytryptamine in Human and Animal Blood Platelets: Monoamine Distribution Between Platelets and Plasma

Prada

Picotti

1979

British J Pharmacology

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150

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The content of adrenaline (Ad), noradrenaline (NA) and dopamine was measured in human, guinea‐pig, cat, rabbit and rat blood platelets by a highly sensitive and specific radioenzymatic method. In all platelet specimens analyzed, the content of the three catecholamines (CA) was several thousand times lower than that of 5‐hydroxytryptamine (5‐HT). In basal conditions, the NA concentration in platelets and plasma always exceeded that of Ad and dopamine. In rat and rabbit platelets, Ad, NA and dopamine were present only in the free (unconjugated) form. Platelets of rats with storage pool deficiency (Fawn‐hooded) contained much less 5‐HT and CA than normal rat platelets. Following restraint stress, platelets of Fawn‐hooded rats, in contrast to normal rat platelets, did not accumulate CA in spite of a dramatic rise in plasma CA. Reserpine, a monoamine depletor, released CA as well as 5‐HT from rabbit platelets in vivo. Subcellular fractionation experiments with rabbit platelets indicate that both CA and 5‐HT are most concentrated in the fraction consisting of pure 5‐HT organelles. Both in humans and rabbits the concentration gradient between platelets and plasma was much lower for CA than for 5‐HT, indicating that a high affinity transport mechanism operates in vivo for 5‐HT but not for CA. In conclusion, the present data show that both human and animal platelets contain Ad, NA and dopamine. The bulk of the CA seems to be stored as unconjugated amines together with 5‐HT, histamine and p‐octopamine in a multitransmitter storage site, namely the 5‐HT organelle.

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EFFECTS OF 5,6‐DIHYDROXYTRYPTAMINE AND 5,7‐DIHYDROXYTRYPTAMINE ON THE RAT CENTRAL NERVOUS SYSTEM AFTER INTRAVENTRICULAR OR INTRACEREBRAL APPLICATION AND ON BLOOD PLATELETS IN VITRO

Cited by 27 publications

References 43 publications

Treatment with a serotonin-depleting regimen of MDMA prevents conditioned place preference to sex in male rats.

Treatment with a serotonin-depleting regimen of MDMA prevents conditioned place preference to sex in male rats.

Differences Between Intracellular Platelet and Brain Proteins that Bind Serotonin

Content and Subcellular Localization of Catecholamines and 5‐hydroxytryptamine in Human and Animal Blood Platelets: Monoamine Distribution Between Platelets and Plasma

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