Effects of 4-acetamido-4′-isothiocyano-2,2′-disulfonic stilbene on ion transport in turtle bladders

Ehrenspeck, Gerhard; Brodsky, William A.

doi:10.1016/0005-2736(76)90268-6

Cited by 78 publications

(34 citation statements)

References 12 publications

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“…Similar conclusions have been suggested by a recent study of in vitro, isolated, perfused, rabbit proximal convoluted tubules (57). The turtle bladder also has a conductive pathway for bicarbonate diffusion across the serosal membrane (32,33), and SITS inhibits anion transport on that side of either tissue (32,33,36). We cannot rule out an effect on the pertibular membrane in our studies because large concentrations of the drugs were used, and some fraction may have been reabsorbed and affected peritubular anion transport.…”

Section: Neutral Sodium Chloride Transportsupporting

confidence: 78%

“…The effects of furosemide upon electrogenic sodium reabsorption were examined in the last series of (27) found that 1 mM furosemide reduced volume reabsorption by 50% in the perfused rat ileum. Similar doses of furosemide (1)(2)(3)(4)(5) mM) also inhibit chloride transport across the erythrocyte membrane (28,29), primarily by inhibiting an anion exchange and transport in many physiological systems (30)(31)(32)(33)(34)(35)(36) …”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Effects of Anion-Transport Inhibitors on NaCl Reabsorption in the Rat Superficial Proximal Convoluted Tubule

Lucci¹,

Warnock²

1979

J. Clin. Invest.

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A B S T R A C T The effects of anion-transport inhibitors on volume reabsorption, and total CO2 concentrations were examined by in vivo microperfusion of superficial proximal convoluted tubules of rats. The luminal perfusion solution was a high-chloride, lowbicarbonate solution like that in the in vivo late proximal tubule. The anion-transport inhibitors were only added to the luminal perfusion solutions.In tubules perfused with the control high-chloride solution, the rate of volume reabsorption (J,) was 2.3±0.2 nl/mm min (n = 18), and the collected total CO2 concentration was 4.0±0.3 mM. Furosemide (3 mM) caused a marked reduction in volume reabsorption to 0.8±0.3 nl/mmmin (n = 20) and only a slight increase in the total CO2 concentration of collected samples of perfusate (7.8±0.5 mM). 0.8 mM acetazolamide caused a more pronounced rise in the collected total CO2 concentrations to 10.7±0.5 mM but only a slight fall in J, to 1.7±0.3 nl/mm-min (n = 19). Hence, we inferred that inhibition of carbonic anhydrase only partially accounted for the inhibition of J, by furosemide. 4-acetamido-4'-iso-thiocyanato-stilbene-2,2'-disulphonic acid (0.1 mM), a well-characterized inhibitor of erythrocyte anion exchange mechanisms, also reduced J, to 1.6±0.3 nl/mm min (n = 15) without changing the total CO2 concentrations of the collected perfusates (3.6±0.4 mM). The effect of 4-acetamidoA4'-iso-thiocyanato-stilbene-2,2'-disulphonicPortions of this work were presented at the 10th Annual

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Section: Neutral Sodium Chloride Transportsupporting

confidence: 78%

Section: Methodsmentioning

confidence: 99%

Effects of Anion-Transport Inhibitors on NaCl Reabsorption in the Rat Superficial Proximal Convoluted Tubule

Lucci¹,

Warnock²

1979

J. Clin. Invest.

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“…brane, due to the presence of band III (the anion channel) which has a very high affinity for DIDS (K i, 0.04/aM [2]) and is ~100-times more abundant than the Ca 2+ ATPase. It is of interest that SITS, an inhibitor of the anion channel very similar to DIDS, has been found to inhibit the Na ÷ + K+-ATPase of microsomal preparations of both turtle bladder and eel electric organ [5] at/aM levels. DIDS has also been found to inhibit the transport of Ca 2+ into liposomes reconstituted with the purified sarcoplasmic reticulum Ca2+-ATPase [6], and to bind the ATPase molecule.…”

Section: Resultsmentioning

confidence: 99%

Inhibition of the purified and reconstituted calcium pump of erythrocytes by μM levels of dids and nap‐taurine

Niggli¹,

Sigel²,

Carafoli³

1982

FEBS Letters

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“…In SITS-treated halves the inhibition of the net sodium absorption was significant (P < 0.01) and increased from 20% at 60 min to 30% at 90 min. Ehrenspeck and Brodsky (14) found no significant inhibition of the net sodium absorption under somewhat different conditions.…”

Section: Sits)mentioning

confidence: 88%

“…Inhibition of anion transport by SITS has been demonstrated in a variety of other tissues as well (19)(20)(21). Ehrenspeck and Brodsky (14) reported that SITS added to the serosal side of the turtle bladder inhibits anion transport as judged from the short-circuit current in sodium-free media. Furthermore, a preliminary report by Ehrenspeck et al (22) suggests that the stilbenes bind to a protein located in the serosal cell membrane.…”

Section: Discussionmentioning

confidence: 99%

Inhibition of the Bicarbonate Exit Step in Urinary Acidification by a Disulfonic Stilbene

Cohen¹,

Mueller²,

Steinmetz³

1978

J. Clin. Invest.

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A B S T R A C T Acidification of the luminal solution by the isolated turtle bladder involves H+ secretion by a pump at the luminal membrane. The OH-dissociated in this process reacts with CO2 and forms HCO-which moves passively out of the cell across the serosal cell membrane. In the present study, this exit step for HCO3 was inhibited by serosal addition of the disulfonic stilbene, SITS, an agent which is thought to bind to a transport protein at the serosal cell membrane. 90 min after serosal addition of 0.5 mM SITS, H+ secretion decreased by > 80%. In contrast, luminal addition of SITS had no effect. During inhibition of H+ secretion by serosal SITS, overall cell pH, measured by the 5,5-dimethyl-2,3-oxazolidinedione method, increased from 7.48+0.03 to 7.61+0.02. This increase of0. 13+0.02 pH U was associated with a much larger regional pH increase as judged from the decrement in the attainable pH gradient across the epithelium. After serosal SITS, this gradient was reduced from 2.88+0.06 to 2.09±0.11 pH U. In the absence of evidence for increased H+ permeability or a change in the force of the H+ pump, the gradient decrement of 0.79±0.08 U reflects a similar pH increment on the cytoplasmic side of the pump.SITS inhibits the exit of bicarbonate across the serosal cell membrane and, thereby, creates a compartment of high alkalinity in series with the pump.

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Effects of 4-acetamido-4′-isothiocyano-2,2′-disulfonic stilbene on ion transport in turtle bladders

Cited by 78 publications

References 12 publications

Effects of Anion-Transport Inhibitors on NaCl Reabsorption in the Rat Superficial Proximal Convoluted Tubule

Effects of Anion-Transport Inhibitors on NaCl Reabsorption in the Rat Superficial Proximal Convoluted Tubule

Inhibition of the purified and reconstituted calcium pump of erythrocytes by μM levels of dids and nap‐taurine

Inhibition of the Bicarbonate Exit Step in Urinary Acidification by a Disulfonic Stilbene

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