Effects of 3 diets with various calcium contents on 24-h energy expenditure, fat oxidation, and adipose tissue message RNA expression of lipid metabolism–related proteins

Boon, Niels; Hul, Gabby B.; Viguerie, Nathalie; Sicard, Audrey; Langin, Dominique; Saris, Wim H. M.

doi:10.1093/ajcn/82.6.1244

Cited by 40 publications

(57 citation statements)

References 51 publications

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“…18,38 However, strikingly, we observed significant differences in the serum concentration of 1,25-OH 2 D 3 , whereas this was not the case in the present investigation. A possible explanation for this discrepancy could be that the baseline values of serum 1,25-OH 2 D 3 in this investigation were markedly lower than those in our previous investigations.…”

Section: Discussioncontrasting

confidence: 89%

“…To verify the results of our two previous investigations, 18,22 we also determined resting fat oxidation, EE and adipose tissue mRNA expression of genes involved in fat metabolism as suggested by Zemel.…”

Section: Introductionmentioning

confidence: 74%

“…The smaller fat binding effect of calcium in all four diets might be the result of the high protein content of the diets. To compare the measurements of EE and fat oxidation and the mRNA expression data of the present investigation to our previous experiment, 18 we choose to use the same relatively high protein content as in that study. This high protein content of the diets was chosen to equalize the macronutrient composition of the experimental diets.…”

Section: Discussionmentioning

confidence: 99%

“…18,38 However, in those studies [35][36][37] the subjects were in a negative energy balance, 36 or the postprandial or acute effects of calcium and dairy intake were studied. 35,37 During energy restriction, EE is decreased and the potential stimulatory effects of a high calcium intake on energy metabolism 39 could counteract this decrease.…”

Section: Discussionmentioning

confidence: 99%

“…15,17 Moreover, no effects of increasing dietary calcium intake on energy expenditure (EE) or fat oxidation were observed in a number of investigations studying humans or rodents in energy balance. 18,19 Interestingly, in one of these investigations, a marked increase in faecal fat excretion (from 6.0 to 14.2 g/day, when calcium intake was increased by B1250 mg/day) was observed on a diet with a normal protein content. 19 An increased calcium intake could lead to an increased calcium concentration in the intestine, which in turn causes an increased binding of fatty acids and bile acids.…”

Section: Introductionmentioning

confidence: 95%

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An intervention study of the effects of calcium intake on faecal fat excretion, energy metabolism and adipose tissue mRNA expression of lipid-metabolism related proteins

et al. 2007

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Context:In various observational studies, an inverse relation between calcium intake and body weight has been observed. A possible explanation could be an increased calcium excretion through the faeces caused by an increased dietary calcium intake. Objective: To examine whether an increased calcium intake could lead to changes in faecal fat and energy excretion. Design: Four different isocaloric diets with various calcium contents (400, 1200 and 2500 mg from dairy and 1200 mg from calcium carbonate (1200S)) were administered in a crossover design for 7 days each. Subjects: Five healthy men and five healthy women (age ¼ 2872, body mass index ¼ 24.170.4, body fat% ¼ 25.672.4) were recruited by local announcement. Measurements: At the end of every intervention period, faecal samples were collected for determination of fat, energy and calcium content, blood samples were obtained for determination of relevant blood parameters; and fat samples were obtained for measurement of the mRNA expression. Furthermore, resting energy expenditure and fat oxidation were measured with the ventilated-hood technique. Results: We observed a non-significant 56% increase in fat excretion (P ¼ 0.159) on the 2500 mg diet, compared to the 400 mg diet. The 2500 mg diet significantly reduced the expression of fatty acid synthase (FAS) mRNA (Po0.05) and the calcium content of the diets significantly affected calcium excretion. Furthermore, we saw a significant decrease of serum triglycerides on the 1200S diet (Po0.05). Conclusion: In this study, we observed a trend towards a higher fat excretion on the high-calcium diet, but this difference failed to reach statistical significance. It is possible that the relatively high protein content of the experimental diets increased calcium absorption from the intestine, thus decreasing the amount of calcium available for binding to fat and eliminating possible effects of dietary calcium on fat excretion. Furthermore, we observed decreases in FAS mRNA expression and serum triglycerides as a result of a high calcium intake.

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Section: Discussioncontrasting

confidence: 89%

Section: Introductionmentioning

confidence: 74%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 95%

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An intervention study of the effects of calcium intake on faecal fat excretion, energy metabolism and adipose tissue mRNA expression of lipid-metabolism related proteins

et al. 2007

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The Role of Dairy Products and Dietary Calcium in Weight Management

Spence

Narasimmon

2009

Weight Control and Slimming Ingredients in Food Technology

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Calcium ameliorates obesity induced by high-fat diet and its potential correlation with p38 MAPK pathway

et al. 2011

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To investigate whether and on which pathway dietary calcium influence the obesity induced by high-fat diet, thirty male Kunming mice were fed in six groups for 4 weeks and mouse preadipocytes were divided into eight groups for different treatment. Body weight gain was measured each week. Calcium in serum and tissues, intracellular free Ca(2+) concentration ([Ca(2+)]i), blood fat and intracellular lipid content were also measured. The expression of Lipid metabolism-related genes were measured by q RT-PCR. Compared with control group, body weight gain (P < 0.05) and fat pad weight (P < 0.01) in Low calcium group decreased. Triglycerides (TG) and total Cholesterol (TC) level decreased (P < 0.01), while HDL-Cholesterol (HDL) level increased (P < 0.01). And calcium supply increased calcium content in blood serum and tissues. In tissues, adipogenesis and vitamin D receptor (VDR) genes expression decreased but lipoclasis genes expression increased. These anti-obesity effects were more obvious when supplying with 2.8% calcium, but the effects were reduced while supplying Nifedipine at the same time. The results in preadipocytes indicated that calcium-treated can reduce intracellular lipid content, along with adipogenesis and lipoclasis genes expression decrease, promoted the expression levels of p38 MAPK pathway upstream gene MKK6 (P < 0.01) and downstream gene MAPKAPK2 (P < 0.01). Treated with SB203580 could increase adipogenesis genes expression, decrease lipoclasis genes expression and ([Ca(2+)]i) (P < 0.01). These results implied that dietary calcium had remarkable effect on anti-obesity effect and p38 MAPK pathway potentially participated in calcium-mediated lipid accumulation and lipolysis in mouse preadipocytes.

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Effects of 3 diets with various calcium contents on 24-h energy expenditure, fat oxidation, and adipose tissue message RNA expression of lipid metabolism–related proteins

Abstract: Altering the dietary calcium content for 7 d does not influence substrate metabolism, energy metabolism, or gene expression in proteins related to fat metabolism, despite significant changes in 1,25(OH)(2)D(3) concentrations.

Cited by 40 publications

References 51 publications

An intervention study of the effects of calcium intake on faecal fat excretion, energy metabolism and adipose tissue mRNA expression of lipid-metabolism related proteins

An intervention study of the effects of calcium intake on faecal fat excretion, energy metabolism and adipose tissue mRNA expression of lipid-metabolism related proteins

The Role of Dairy Products and Dietary Calcium in Weight Management

Calcium ameliorates obesity induced by high-fat diet and its potential correlation with p38 MAPK pathway

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