Effects of 3% Boric Acid Solution on Cutaneous Candida albicans Infection and Microecological Flora Mice

Liu, Qing; Zhao, Liang; Zhang, Changlin; Xu, Yingjiang; Li, Xiaojing; Gao, Hongqi

doi:10.3389/fmicb.2021.709880

Cited by 8 publications

(8 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The effective rate of 3% BAS was 83%, and that of SWFI was 25%, which may be caused by discrepancies in drug treatment, operation during treatment, or other factors. Compared with SWFI, 3% BAS had a therapeutic effect and was statistically significant (Liu et al, 2021 ). However, the mechanism is not entirely clear.…”

Section: Discussionmentioning

confidence: 99%

“…From each group, we randomly selected 6 animals for model verification. Please refer to our published articles for details about the CA mycelium suspension configuration, model establishment, and verification method (Liu et al, 2021 ).…”

Section: Methodsmentioning

confidence: 99%

“…Active dendritic cells and macrophages secrete IL-23, which promotes the increase in the number of Th17 cells and promotes the production of IL-17 and IL-22 (Ge et al, 2019 ). Our previous studies showed that 3% boric acid solution (BAS) treats CA infections in mouse skin (Liu et al, 2021 ); however, the specific mechanism of action has not been thoroughly studied. Therefore, we hypothesized whether BAS increased IL-17, IL-22, and IL-23 in mice, helping the host resist CA infection.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Boric Acid Solution Inhibits Candida albicans Infections in Mouse Skin via the IL-23/Th17 Axis

Zhao

Liu

et al. 2022

Front. Microbiol.

Self Cite

View full text Add to dashboard Cite

The purpose of this study was to investigate the effect and mechanism of 3% boric acid solution (BAS) against Candida albicans (CA) infection via the interleukin-23 (IL-23)/T helper 17 cell (Th17) axis. 36 female mice were randomly divided into 3 groups, and 2 injection sites on the back of the mice were chosen at random. Group N was injected with sterile water for injection (SWFI), and Group M and Group B were injected with CA mycelium suspension. After successful model verification, the remaining mice entered the following treatments 5 days later. Group B was treated with 3% BAS, Group M was treated with SWFI, and Group N was not treated. Levels of interleukin-17 (IL-17), IL-22, and IL-23 in mouse blood were measured on days 1, 3, 5, and 7 of treatment. On day 7, IL-17, IL-22, and IL-23 in mouse skin were detected. Serum levels of IL-17, IL-22, and IL-23 in Group M were higher than in Group N on the first day of treatment (p < 0.05). Expression levels of IL-17, IL-22, and IL-23 in the epidermis of the skin lesions in Group M were higher than in Group N on day 7 (p < 0.05). The serum level of IL-17 in Group B was higher than in Group M on days 5 and 7 (p < 0.05). Serum levels of IL-22 in Group B on days 1, 5, and 7 were higher than in Group M (p < 0.05). Serum levels of IL-23 in Group B were higher than in Group M on days 3, 5, and 7 (p < 0.05). IL-17 and IL-23 in Group B reached a peak on day 5, significantly different on days 1, 3, and 7 (p < 0.05). The expression intensity of IL-17, IL-22, and IL-23 in the skin lesions of Group B was higher than that of Group M on day 7 (p < 0.05). We conclude that IL-17, IL-22, and IL-23 are involved in the anti-CA activity in mouse skin, and 3% BAS increased IL-17, IL-22, and IL-23 to mediate these effects.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Boric Acid Solution Inhibits Candida albicans Infections in Mouse Skin via the IL-23/Th17 Axis

Zhao

Liu

et al. 2022

Front. Microbiol.

Self Cite

View full text Add to dashboard Cite

show abstract

“…For assessment of colony formation, each group of the skin tissues was placed in 200 μL of double-distilled water, homogenized, and centrifugated at 6000× g at room temperature for 5 min [ 28 ]. The supernatants were collected, and adjusted with double-distilled water to 1 mL.…”

Section: Methodsmentioning

confidence: 99%

Fungicidal Activity of AP10W, a Short Peptide Derived from AP-2 Complex Subunit mu-A, In Vitro and In Vivo

Gong

et al. 2022

Biomolecules

View full text Add to dashboard Cite

With the increase in the incidence of fungal infections, and the restrictions of existing antifungal drugs, the development of novel antifungal agents is urgent. Here we prove that AP10W, a short peptide derived from AP-2 complex subunit mu-A, displays conspicuous antifungal activities against the main fungal pathogens of human infections Candida albicans and Aspergillus fumigatus. We also show that AP10W suppresses the fungal biofilm formation, and reduces the pre-established fungal biofilms. AP10W appears to exert its fungicidal activity through a mode of combined actions, including interaction with the fungal cell walls via laminarin, mannan and chitin, enhancement of cell wall permeabilization, induction of membrane depolarization, and increase in intracellular ROS generation. Importantly, we demonstrate that AP10W exhibits little toxicity towards mammalian fibroblasts, and effectively promotes the healing of wounded skins infected by C. albicans. These together indicate that AP10W is a new member of fungicidal agents. It also suggests that AP10W has a considerable potential for future development as a novel antifungal drug.

show abstract

“…Denitrification is a gradual reduction of nitrate nitrogen into nitrite nitrogen, and eventually into gaseous nitrogen, thus reducing the N stock in the system [29]. The addition of nitrifying bacteria (living bacteria at 3.67 × 10 7 Cfu/mL) to water can significantly reduce the ammonia nitrogen content in water body [31]. The application of denitrifying bacteria (10 5 Cfu/mL) exhibited a good removal effect on nitrogen [32].…”

Section: Introductionmentioning

confidence: 99%

Nitrogen Removal of Water and Sediment in Grass Carp Aquaculture Ponds by Mixed Nitrifying and Denitrifying Bacteria and Its Effects on Bacterial Community

Han

et al. 2022

Water

View full text Add to dashboard Cite

Nitrification and denitrification are important for nitrogen (N) cycling in fish ponds culture, but the effects of nitrifying and denitrifying bacteria concentrations on pond water and sediments remain largely unknown. Here, we used 0, 0.15, 0.30, 0.60 mg/L different concentrations of mixed nitrifying and denitrifying bacteria to repair the pond substrate through an enclosure experiment lasting 15 days. The results showed that the purification effect of nitrifying and denitrifying bacteria was most obvious on pond nitrogen from day 4 to day 7. The optimal relative concentration was 0.60 mg/L for nitrifying and denitrifying bacteria; NH4+-N (ammonia nitrogen) decreased by 75.83%, NO2−-N (nitrite) by 93.09%, NO3−-N (nitrate) by 38.02%, and TN (total nitrogen) by 45.16% in this concentration group on pond water. In one cycle, C/N (carbon/nitrogen) ratio of both water body and bottom sediment significantly increased, but C/N ratio of water body increased more significantly than that of sediment. Water C/N ratio increased by 76.00%, and sediment C/N ratio increased by 51.96% in the 0.60 mg/L concentration group. Amplicon sequencing of pond sediment showed that the change in nitrifying and denitrifying bacterium diversity was consistent with that in water quality index. Dominant nitrifying bacteria had a relatively high percentage, with significant differences in dominant bacterium percentage across different bacterial addition groups, while dominant denitrifying bacterium percentage was not high without significant differences among different groups. The dominant species of nitrifying bacteria were, respectively, Nitrosomonas, Nitrosovibrio, Nitrosospira, and Aeromonas, and the dominant species of denitrifying bacteria were Thauera, Azoarcus, Magnetospirillum, Azospira, and Idiomarina. The correlation analyses showed an aerobic nitrification and facultative anaerobic denitrification in pond sediments. Research shows that the addition of exogenous nitrifying and denitrifying bacteria can effectively reduce the nitrogen load of pond water and sediment. At the concentration of 0.6 mg/L, the nitrogen load of pond water and sediment decreased most obviously, which had the best effect on pond purification.

show abstract

Effects of 3% Boric Acid Solution on Cutaneous Candida albicans Infection and Microecological Flora Mice

Cited by 8 publications

References 28 publications

Boric Acid Solution Inhibits Candida albicans Infections in Mouse Skin via the IL-23/Th17 Axis

Boric Acid Solution Inhibits Candida albicans Infections in Mouse Skin via the IL-23/Th17 Axis

Fungicidal Activity of AP10W, a Short Peptide Derived from AP-2 Complex Subunit mu-A, In Vitro and In Vivo

Nitrogen Removal of Water and Sediment in Grass Carp Aquaculture Ponds by Mixed Nitrifying and Denitrifying Bacteria and Its Effects on Bacterial Community

Contact Info

Product

Resources

About