Effects of 28-homobrassinolide on nickel uptake, protein content and antioxidative defence system in Brassica juncea

Abstract

The effects of 28-homobrassinolide (HBL) on nickel uptake, protein content and activities of antioxidative enzymes were determined in the seedlings of Brassica juncea L. The seeds were treated with different concentrations (0, 0.01, 1 and 100 nM) of HBL for 8 h and then sown in the Petri plates containing various concentrations (0, 25, 50 and 100 mg dm -3 ) of nickel. After 7 d, observations were made on shoot and root length, Ni uptake, protein content and activities of antioxidative enzymes (guaiacol peroxidase, catalase, glutathione reductase, ascorbate peroxidase and superoxide dismutase). The growth of seedlings was inhibited by Ni, however, less after HBL pre-treatment. The protein content and antioxidative enzyme activities were also increased by HBL treatment.Additional key words: antioxidative enzymes, brassinosteroids, heavy metal toxicity, Indian mustard.

Effect of 24-epibrassinolide on ROS content, antioxidant system, lipid peroxidation and Ni uptake in Solanum nigrum L. under Ni stress

“…However, this result contrast with previous observations of decreased SOD activity in wheat shoots (Gajewska et al, 2006b) and Alyssus bestolonii and Nicotiana tabacum subjected to Ni stress (Boominathan and Doran, 2002). In accordance to our results, where the pretreatment with 24-EBL also led to a higher SOD activity when compared to the control, Sharma et al (2008) reported that Ni caused an increment of SOD activity in Brassica juncea plants both in the presence and absence of 28-homobrassinolide. Although Ni did not alter the isoenzyme pattern of SOD in S. nigrum, it was possible to relate the rise in total SOD activity with the increase of all SOD isoenzymes in Ni exposed plants as it is quite clear by the analyses of SOD activity zymograms.…”

“…Mustard plants grown under Ni stress and treated with 28-homobrassinolide exhibited an enhanced activity of CAT and peroxidases (Alam et al, 2007), a result which has been confirmed by the works performed by Ali et al (2008b) and Sharma et al (2008) with Brassica juncea. However, overall our results revealed that plants exposed…”

Effect of Brassinosteroid and Selenium on Uptake and Accumulation of Chromium in Yellow Flag (Iris pseudacorus)

“…6). Many previous studies reported that Br24 and Br28 could reduce the concentrations of metal(loid)s (Ni, Zn, Cu and B) and alleviate their toxicity in plants (Sharma and Bhardwaj, 2007;Sharma et al, 2008;Kanwar et al, 2013;Ramakrishna and Rao, 2013;Surgun et al, 2016). Their results suggested that Br (Br24 and Br28) could decrease metal (loid)s uptake by plants via enhancing the activities of glutathione metabolism and biosynthesis and antioxidant enzymes.…”

Steroids: A Diverse Class of Secondary Metabolites

“…Brassinosteroids: Brassinosteroids (BRs), such as brassinolide 49, have been shown to elicit a broad spectrum of responses including the promotion of cell elongation and cell division, inhibition of deetiolation in the dark, repression of light-regulated genes in the dark and repression of stress-regulated genes [77]. In view of their structural similarities with animal steroids, it has been proposed that BRs might interact with a soluble receptor in order to regulate the expression of specific genes [78]. The BRs tend to counter biotic as well as abiotic stress in plants [79].…”