Effects of 25-hydroxycholecalciferol and soy isoflavones supplementation on bone mineralisation of quail

Abstract

1. The effects of 25-hydroxycholecalciferol (25-OH-D(3)) and soy isoflavones supplementation on performance, carcase recovery, bone mineral density, and tibia ash, Ca, P, and serum vitamin D concentrations and alkaline phosphatase activity in the Japanese quail (Coturnix coturnix japonica) exposed to high ambient temperature were evaluated. 2. A total of 270 ten-d-old Japanese quail were randomly assigned to 9 treatment groups, 6 replicates of 5 birds each in a 2 x 3 x 3 factorial arrangement of treatments. Birds were kept in a temperature-controlled room at either 22 degrees C (thermo-neutral) or 34 degrees C (heat stress) for 8 h/d (09:00-17:00 h) and given a basal (control) diet or the basal diet supplemented with one of three levels of 25-OH-D(3) (0, 250 and 500 IU/kg of diet) combined with one of three levels of soy isoflavones (0, 400 and 800 mg/kg of diet). 3. Birds kept at 34 degrees C consumed less feed and gained less weight than control birds. An increase in body weight, feed intake (and improvement in feed efficiency and carcase recovery were found in soy isoflavones and 25-OH-D(3)-supplemented quail reared under heat stress conditions. Bone mineral density, tibia ash, Ca, and P were linearly improved by 25-OH-D(3) and soy isoflavones supplementation in both thermoneutral and heat stress groups. Serum vitamin D levels and alkaline phosphatase activity were improved by 25-OH-D(3) and soy isoflavones supplementation in both thermoneutral and heat stress groups in quail. 4. In conclusion, a combination of 25-OH-D(3) and soy isoflavones supplementation to basal diet significantly improved bone mineralisation in quail.

Effect of different vitamin D sources and calcium levels in the diet of layers in the second laying cycle

“…This result may be attributed to the fact that the activity of cholecalciferol, 25(OH)D 3 , and 1,25(OH) 2 D 3 depend on the biological response of birds (Aburto et al, 1998). Sahin et al (2009) obtained better bone mineralization in laying quails when 25(OH)D 3 was added to the diet.…”

Quercetin Regulates Calcium and Phosphorus Metabolism Through the Wnt Signaling Pathway in Broilers

“…Calcium concentration in tibia was determined by an atomic absorption spectrophotometer, and P was determined by the vanadium molybdate colorimetric method. The ash content of tibia was expressed as ash per unit tibial length, and expressed as a percentage of dry weight (28). BMD in tibia was measured using a Qdr-4500w dual energy X-ray bone mineral density detector.…”

The clinical use of vitamin D metabolites and their potential developments: a position statement from the European Society for Clinical and Economic Aspects of Osteoporosis and Osteoarthritis (ESCEO) and the International Osteoporosis Foundation (IOF)

“…A recent study carried out in young healthy Gambian men to asses the effect on serum 25(OH)D after administration of a single dose of calcidiol Recent animal studies have shown that calcidiol is better absorbed in the intestine than cholecalciferol when added to animal feed (i.e., nutrient and/or supplement). This is further underlined by greater effects of calcidiol in reproductive performance, immune protection against infections, and skeletal turnover markers as compared to parental vitamin D3 in vivo in animals [77][78][79]. Recently, calcidiol supplementation in pregnant gilts, in addition to the control diet containing cholecalciferol, has been shown to improve feral skeletal muscle development and myoblast activity, likely improving postnatal muscle growth [80].…”