Effects of 200 Gy 60Co-γ Radiation on the Regulation of Antioxidant Enzymes, Hsp70 Genes, and Serum Molecules of Plutella xylostella (Linnaeus)

Abstract

The diamondback moth, Plutella xylostella (Linnaeus), is one of the notorious pests causing substantial loses to many cruciferous vegetables across the nations. The effects of 60Co-γ radiation on physiology of P. xylostella were investigated and the results displayed that 200 Gy irradiation significantly alters the antioxidant enzyme regulation in six-day-old male pupae of P. xylostella. First, in our research, we detected Oxidase system and stress response mechanism of irradiated pupae, the results displayed that 200 Gy irradiation significantly alters the antioxidant enzyme regulation in six-day-old male pupae of P. xylostella. The levels of superoxide dismutase (SOD) and catalase (CAT) were increased significantly in contrast the level of peroxidase (POD) and glutathione S-transferase (GST) were decreased in 12–24 h post-treatment. The heat shock proteins (Hsps) gene expression level was significant increasing, maximum > 2-folds upregulation of genes were observed in peak. However, they also had a trend of gradual recovery with development. Second, we detected the testis lactate dehydrogenase (LDH) and acid phosphatase (ACP) activity found that in male adults testis they increased significantly than control during its development. Thus the present research investigation highlights that the 60Co-γ radiation treatments alters the physiological development of diamondback moth. The results showed that 200 Gy dosage resulted in stress damage to the body and reproductive system of the diamondback moth.

Crocetin Mitigates Irradiation Injury in an In Vitro Model of the Pubertal Testis: Focus on Biological Effects and Molecular Mechanisms

“…The data obtained using various models including cells, animals, and recently humans suggest that radioprotective agents working through a variety of mechanisms have the potential to decrease free radical damage produced by IR [ 15 ]. Therefore, recently, much interest has been ignited to discover antioxidants which would counteract or minimize radiation-induced testicular toxicity [ 16 , 17 , 18 , 19 , 20 , 21 , 22 ].…”

Study of the dual biological impacts of aqueous extracts of normal and gamma-irradiated Galleria mellonella larvae

“…36 In the present study, the protease, lipase, and acid phosphatase enzymes produced by the G. mellonella larvae reached the maximal level at 6 Gy. 37 Wenlong et al. reported that 200 Gy irradiation disrupted the enzyme levels of P. xylostella , leading to body damage, while long-wave ultraviolet light was reported to increase oxidative enzymes in Dendrolimus tabulaeformis .…”

Schizophyllum commune induced oxidative stress and immunosuppressive activity in Spodoptera litura

“…GST as a detoxifying enzyme effectively metabolizes the exogenous toxic compounds and plays a crucial role in providing defense as well as maintaining the normal physiological activities in the body [52][53][54]. The enzyme catalyzes the harmful compounds with glutathione and assists their discharge from the body in a non-enzymatic fashion [55].…”