Effects of 2-methoxyestradiol on hydrogen peroxide induced neuronal cell death and tau hyperphosphorylation

Zacharia, Lefteris C.; Eleftheriou, Constantina; Gkretsi, Vasiliki

doi:10.1016/j.lfs.2022.121047

Cited by 2 publications

(1 citation statement)

References 26 publications

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“…Renewed the media every 2 days. Approximately 80% of cells confluence was detached by using 0.25% trypsin–EDTA for further cell culture and biochemical assay (Zacharia et al., 2022).…”

Section: Methodsmentioning

confidence: 99%

Synthesis, characterization, and anti‐inflammatory properties of novel ethyl 3‐benzoyl‐7‐(trifluoromethyl)indolizine‐1‐carboxylate derivatives: In silico and in vitro analysis

Singh,

Kushwaha,

Tandon

et al. 2024

Chem Biol Drug Des

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Series of 7‐(Trifluoromethyl) substituted indolizine 4a‐g was synthesized using the one‐pot method. Spectroscopic techniques such as IR, 1H‐NMR, 13C‐NMR, and HRMS were used for the structure confirmation of newly synthesized compounds. These 4a‐g compounds were tested for their anti‐inflammatory activity. In this study, we identified novel indolizine derivative compounds 4a‐g selectively targeting COX‐2 enzyme, tumor necrosis factor‐α (TNF‐α) and, interleukin‐6 (IL‐6). The in silico docking studies of 4a‐g showed that these compounds have a higher affinity for COX‐2 enzyme, TNF‐ α, and IL‐6. In silico ADME profile analysis predicts that these compounds have good gastrointestinal tract and blood–brain barrier absorption. In vitro studies showed that compound 4d significantly reduces the level of COX‐2 enzymes as compared to indomethacin. Compounds 4e, 4f, and 4a were also found to significantly reduce the level of TNF‐α, while compounds 4f, 4g, and 4d, showed a reduction in the level of IL‐6 when compared to indomethacin. Compounds 4a, 4d, and 4f also reduces nitric oxide (NO) level, compared to indomethacin. Overall, the current study illustrates significant anti‐inflammatory activities of these novel 7‐(Trifluoromethyl) substituted indolizine derivatives.

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“…Renewed the media every 2 days. Approximately 80% of cells confluence was detached by using 0.25% trypsin–EDTA for further cell culture and biochemical assay (Zacharia et al., 2022).…”

Section: Methodsmentioning

confidence: 99%

Synthesis, characterization, and anti‐inflammatory properties of novel ethyl 3‐benzoyl‐7‐(trifluoromethyl)indolizine‐1‐carboxylate derivatives: In silico and in vitro analysis

Singh,

Kushwaha,

Tandon

et al. 2024

Chem Biol Drug Des

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Beyond boundaries: Neuroprotective effects of steroids and ecdysteroids in SH‐SY5Y cells ‐ A systematic review

Tayeb,

Njangiru,

Minorics

2024

Neuroprotection

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Steroids and ecdysteroids have been shown to exhibit a range of biological effects, including anti‐inflammatory, anticancer, and neuroprotective. This systematic review aims to highlight the evidence‐based neuroprotective and antioxidant effects of steroids and ecdysteroids in SH‐SY5Y neuroblastoma cells. A comprehensive literature search was conducted on May 11, 2023, without publication source restrictions, using various electronic databases, including PubMed, Web of Science (WoS), Scopus, and Cumulative Index to Nursing and Allied Health Literature. Of 103 articles, only 20 studies were included for investigating the neuroprotective effects of steroids and ecdysteroids in SH‐SY5Y cells exposed to oxidative stress or neurotoxic agents. The risk of bias and quality assessment of the included studies were evaluated in accordance with the Nature Publication Quality Improvement Project specific criteria. The selected studies reported the antioxidant effects of the tested compounds on SH‐SY5Y cells and demonstrated their ability to scavenge free radicals and prevent lipid peroxidation. These findings suggest that neurosteroids have potential therapeutic applications for the prevention and treatment of neurodegenerative diseases characterized by oxidative stress and neuronal damage. Some studies have investigated the molecular mechanisms underlying the neuroprotective and antioxidant effects of steroids and ecdysteroids in SH‐SY5Y cells. These mechanisms include the activation of antioxidant enzymes, such as superoxide dismutase and glutathione peroxidase, and the modulation of various signaling pathways, including the phosphoinositide 3‐kinase/protein kinase B and mitogen‐activated protein kinase/extracellular signal‐regulated kinase pathways. This review provides evidence that the tested compounds have remarkable neuroprotective and antioxidant effects in human neuroblastoma SH‐SY5Y cells.

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Effects of 2-methoxyestradiol on hydrogen peroxide induced neuronal cell death and tau hyperphosphorylation

Cited by 2 publications

References 26 publications

Synthesis, characterization, and anti‐inflammatory properties of novel ethyl 3‐benzoyl‐7‐(trifluoromethyl)indolizine‐1‐carboxylate derivatives: In silico and in vitro analysis

Synthesis, characterization, and anti‐inflammatory properties of novel ethyl 3‐benzoyl‐7‐(trifluoromethyl)indolizine‐1‐carboxylate derivatives: In silico and in vitro analysis

Beyond boundaries: Neuroprotective effects of steroids and ecdysteroids in SH‐SY5Y cells ‐ A systematic review

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