Effects of 2-hydroxy-4-methylthiobutyrate on portal plasma flow and net portal appearance of amino acids in piglets

Fang, Zhengfeng; Luo, Jie; Zhang, Qi; Huang, Feiruo; Zhao, Shengjun; Liu, M. Y.; Jiang, Siwen; Peng, Jian

doi:10.1007/s00726-008-0110-1

Cited by 27 publications

(44 citation statements)

References 31 publications

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“…In another part of this study, we reported that the fractional portal balance of L-methionine over the 6 h period was not different between the DL-HMTB and DL-MET groups (13) . However, because it was not known how much D-MET or DL-HMTB was converted to L-methionine when they were transported across the enterocytes, the input of L-methionine in the calculation of the fractional portal balance was estimated according to the total methionine (dietary L-methionine plus D-MET or DL-HMTB).…”

Section: Discussionmentioning

confidence: 58%

“…Particularly, compared with DL-MET-fed pigs, pigs fed the DL-HMTB diet had increased net portal balances of leucine, isoleucine, histidine, arginine and alanine, but had a decreased portal appearance of glutamate over a 6 h period (13) . Therefore, a second endpoint in the present study was to determine whether the changed metabolism of dietary amino acids was also reflected in the change of concentrations of metabolites deriving from the metabolism of these amino acids.…”

Section: Discussionmentioning

confidence: 89%

“…From 08.00 to 14.00 hours, pigs were offered meals at hourly intervals, and the meal was the equivalent of one twenty-fourth of the daily intake (45 g/kg BW). Immediately after the first meal, piglets received a constant infusion of [1-13 C]methionine, suspended in water via the gastric catheter at a rate of about 0·25 ml/min to provide [1][2][3][4][5][6][7][8][9][10][11][12][13] C]methionine at 7·0 mmol/kg BW per h. After the tracer infusion was started, the piglets continued to receive hourly meals. Arterial and portal blood samples were taken at hourly intervals until 6 h of tracer infusion, and all the blood samples were immediately placed on ice.…”

Section: Infusion Protocol and Blood Collectionmentioning

confidence: 99%

“…p-Aminohippurate analysis pAH in plasma samples was analysed as described (13) . The portal plasma flow (PPF) rate (litres/kg per h) was calculated as the following equation:…”

Section: Collection Of Tissue Samplesmentioning

confidence: 99%

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Effects ofdl-2-hydroxy-4-methylthiobutyrate on the first-pass intestinal metabolism of dietary methionine and its extra-intestinal availability

et al. 2010

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The present study was conducted in a one-factorial arrangement to determine the effects of DL-2-hydroxy-4-methylthiobutyrate (DL-HMTB) on the first-pass intestinal metabolism of dietary methionine and its extra-intestinal availability. Barrows (n 6; aged 35 d; weight 8·6 kg), implanted with arterial, portal, mesenteric and gastric catheters, were fed a diet containing DL-methionine (DL-MET) or DL-HMTB once hourly and infused intramesenterically with 1 % p-aminohippurate and intragastrically with [1-13 C]methionine at 7·0 mmol/kg body weight per h. Arterial and portal blood samples were taken at hourly intervals until 6 h of tracer infusion and pigs was then killed for collection of muscle, intestine, liver and kidney samples. The net portal appearance of methionine, expressed as the fraction of ingested directly available L-methionine, was higher (P,0·05) in the DL-HMTB than in the DL-MET diet, and there was no difference (P¼ 0·26) in the fractional portal balance of [1-13 C]methionine between the diets. [1-13 C]methionine enrichment (tracer:tracee ratio; mol/100 mol amino acid) in the jejunum, arterial and portal plasma, liver, kidney and muscle was also not different (P.0·05) between the groups. Over the 6 h period after the start of feeding, the average concentration of citrulline both in the arterial and portal plasma was higher (P,0·05) in the DL-HMTB than in the DL-MET group, and arterial plasma ornithine and taurine concentration was also higher (P,0·05) in the DL-HMTB than in the DL-MET group. However, plasma urea concentration both in the arterial and portal vein was lower (P, 0·05) in the DL-HMTB than in the DL-MET group. These results suggested that the potential difference in the first-pass use of methionine by the intestine between the DL-HMTB and DL-MET diets might affect intestinal and systemic metabolism of other amino acids, which may provide new important insights into nutritional efficiency of different methionine sources.DL-2-Hydroxy-4-methylthiobutyrate: First-pass intestinal metabolism: Extra-intestinal availabilityThe small intestine is a highly differentiated and complex organ, which is not only responsible for the terminal digestion and absorption of nutrients, but also plays an important role in the synthesis, conversion and catabolism of amino acids (1) . Because the apical and basolateral membranes of each enterocyte are chemically, biochemically and physically distinct (2) , an enterocyte can selectively receive nutrients from two sources: the arterial blood across its basolateral membrane and the intestinal lumen across its brush-border membrane. Historically, it is assumed that the primary fate of essential amino acids is to protein synthesis in the target organs; however, intriguing data have demonstrated that catabolism dominates the first-pass utilisation of these amino acids by the gut (3) . Because the extensive catabolism of dietary essential amino acids by the small intestine results in a decrease in their nutritional efficiency (1,3,4) , the question whether the catabolism ...

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Section: Discussionmentioning

confidence: 58%

Section: Discussionmentioning

confidence: 89%

Section: Infusion Protocol and Blood Collectionmentioning

confidence: 99%

“…p-Aminohippurate analysis pAH in plasma samples was analysed as described (13) . The portal plasma flow (PPF) rate (litres/kg per h) was calculated as the following equation:…”

Section: Collection Of Tissue Samplesmentioning

confidence: 99%

See 2 more Smart Citations

Effects ofdl-2-hydroxy-4-methylthiobutyrate on the first-pass intestinal metabolism of dietary methionine and its extra-intestinal availability

et al. 2010

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“…pAH in plasma samples was analyzed as described (15). The portal plasma flow rate was calculated as the following equation: PPF = C i Â IR Â [(pAH p -pAH a ) 3 BW] -1 where PPF is portal plasma flow rate (L 3 kg -1 3 h -1 ), C i is the concentration of infused pAH solution (g/L), IR is the infusion rate (L/h) of pAH, pAH p , and pAH a are the concentration (g/L) of pAH detected in the portal vein and artery, respectively, and BW is the bodyweight (kg).…”

Section: Methodsmentioning

confidence: 99%

Methionine Metabolism in Piglets Fed dl-Methionine or Its Hydroxy Analogue Was Affected by Distribution of Enzymes Oxidizing These Sources to Keto-Methionine

Fang

Luo

Wei

et al. 2010

J. Agric. Food Chem.

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Previous evidence shows that the extensive catabolism of dietary essential amino acids (AA) by the intestine results in decreased availability of these AA for protein synthesis in extraintestinal tissues. This raises the possibility that extraintestinal availability of AA may be improved by supplying the animal with an AA source more of which can bypass the intestine. To test this hypothesis, six barrows (35-day-old, 8.6 +/- 1.4 kg), implanted with arterial, portal, and mesenteric catheters, were fed a DL-methionine (DL-MET) or DL-2-hydroxy-4-methylthiobutyrate (DL-HMTB) diet once hourly and infused intramesenterically with 1% p-amino hippurate. Although the directly available L-MET in DL-MET diet was about 1.2-fold that in DL-HMTB diet, the net portal appearance of L-MET was not different between the two diets. Compared with the low mRNA abundance and low activity of D-2-hydroxy acid dehydrogenase (D-HADH) and l-2-hydroxy acid oxidase (L-HAOX) in the intestine, the high mRNA abundance and high activity of D-AA oxidase (D-AAOX) indicated that the intestine had a relatively higher capacity of D-MET utilization than of dl-HMTB utilization to L-MET synthesis and its subsequent metabolism. However, in contrast to the much lower D-AAOX activity (nmol/g tissue) in the stomach than in the liver and kidney, both d-HADH and L-HAOX activity in the stomach was comparable with those in the liver and/or kidney, indicating the substantial capacity of the stomach to convert DL-HMTB to L-MET. Collectively, the difference in distribution of activity and mRNA abundance of D-AAOX, D-HADH, and L-HAOX in the piglets may offer a biological basis for the similar portal appearance of L-MET between DL-MET and DL-HMTB diets, and thus may provide new important insights into nutritional efficiency of different L-MET sources.

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Effects of maternal exposure to procymidone on hepatic metabolism in the offspring of mice

Wang

Jin

et al. 2023

Environmental Toxicology

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As an effective fungicide widely used in agricultural production, the excessive procymidone (PRO) residue has been detected in the environment and food. Our previous study demonstrated that PRO could destroy the intestinal barrier in mice and has a joint toxic effect. To explore the cross-generational impact of maternal exposure, 10-week-old C57BL/6 female mice were orally administrated to 10 and 100 mg/kg body weight/day of PRO during pregnancy and lactation. The offspring obtained nutrients from the maternal through the placenta and breast milk, and PRO residues were detected in the liver, intestine, and feces of F1 generation. Fecal examination found that the residual PRO had been completely metabolized when the offspring mice grew to 35 days. The drug residue of F1 generation male mice was higher than that of female mice. We attributed this result to the difference in cytochrome P450 (CYP450) enzyme expression between male and female mice. The transcriptional levels of CYP1A1, CYP1A2, CYP2D9, and CYP3A4, and CYP450 protein expression levels, were higher in female mice. Furthermore, targeted MS of plasma revealed abnormal amino acid levels. In addition, PRO-induced hepatic metabolite changes in F0 and F1-7w mice. KEGG pathway analysis further showed that PRO jointly changed the amino acid biosynthesis pathway of the maternal and offspring. In summary, these results indicated that maternal exposure to PRO during a special period would interfere with self metabolism, and offspring will also have metabolic disorders.

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Effects of 2-hydroxy-4-methylthiobutyrate on portal plasma flow and net portal appearance of amino acids in piglets

Cited by 27 publications

References 31 publications

Effects ofdl-2-hydroxy-4-methylthiobutyrate on the first-pass intestinal metabolism of dietary methionine and its extra-intestinal availability

Effects ofdl-2-hydroxy-4-methylthiobutyrate on the first-pass intestinal metabolism of dietary methionine and its extra-intestinal availability

Methionine Metabolism in Piglets Fed dl-Methionine or Its Hydroxy Analogue Was Affected by Distribution of Enzymes Oxidizing These Sources to Keto-Methionine

Effects of maternal exposure to procymidone on hepatic metabolism in the offspring of mice

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