Effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin on vitamin A metabolism in mice

Yang, Yanmei; Huang, Dongyang; Liu, Gefei; Zhong, Jiuchang; Du, Kun; Li, Yifan; Song, Xuhong

doi:10.1002/jbt.20097

Cited by 13 publications

(3 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Interestingly ziprasidone was reported to have favorable effects on total cholesterol, LDL, and HDL levels in patients [50] , which, in context with our results, may be due to altered ABCA1 / AOX1 interaction related modulation of HDL-cholesterol levels. On the other hand, AOX1 was shown to be a dioxin inducible gene [51] . This effect may be due to AHR-mediated downregulation of PPAR [52] , as AOX1 mRNA and protein expression were recently shown do be suppressed by PPAR agonists [53] .…”

Section: Discussionmentioning

confidence: 99%

Human Aldehyde Oxidase 1 Interacts with ATP-binding Cassette Transporter-1 and Modulates its Activity in Hepatocytes

Sigrüener¹,

Buechler²,

Orsó³

et al. 2007

Horm Metab Res

View full text Add to dashboard Cite

AOX1, a member of the cytosolic molybdenum hydroxylase family, has been identified by us earlier as an ABCA1-interacting protein. AOX1 is well-described as xenobiotic metabolizing enzyme, which upon oxidation of acetaldehyde and retinaldehyde to acetic acid and retinoic acid generates reactive oxygen species. Here we show that knock-down of AOX1 in HepG2 by small interfering RNA significantly reduced ABCA1-dependent lipid efflux and enhanced phagocytic uptake of microspheres similar to ABCA1 deficiency, without affecting ABCA1 mRNA and protein levels. ABCA1 and AOX1 are coexpressed in human hepatocytes, kidney proximal tubular epithelial cells, Leydig, and adrenocortical cells. Expression of ABCA1 and AOX1 was investigated by immunohistochemistry in liver tissue arrays. A strong AOX1 expression was found in normal liver, and in cirrhosis. In contrast, hepatocellular carcinomas showed either a complete loss or reduced expression of AOX1. Significant correlations were found between reduced AOX1 expression and tumor stage, or metastatic or regional lymph node states. Deregulation was also observed for ABCA1 expression but to a lesser extent. Our findings show that the interaction of ABCA1 with AOX1 modulates ABCA1-linked cellular functions such as lipid efflux and phagocytosis in hepatocytes, and the reduced expression of AOX1 in malignant transformed hepatocytes supports the differentiation dependent upregulation of AOX1.

show abstract

Section: Discussionmentioning

confidence: 99%

Human Aldehyde Oxidase 1 Interacts with ATP-binding Cassette Transporter-1 and Modulates its Activity in Hepatocytes

Sigrüener¹,

Buechler²,

Orsó³

et al. 2007

Horm Metab Res

View full text Add to dashboard Cite

show abstract

“…Residues were dissolved in 1 ml acetonitrile for each tube. Resuspended samples were analyzed immediately by HPLC using conditions as previously described ( 47 , 48 ). Briefly, HPLC was carried out on the Shimadzu LC solution system with Inertsil ® ODS-SP column (5 µ m, 4.6×150 mm) and mobile phase comprising 30 mmol/l ammonium acetate/acetonitrile (15:85, v/v).…”

Section: Methodsmentioning

confidence: 99%

Regulation of retinoic acid synthetic enzymes by WT1 and HDAC inhibitors in 293 cells

Wang

et al. 2017

International Journal of Molecular Medicine

View full text Add to dashboard Cite

All-trans retinoic acid (atRA), which is mainly generated endogenously via two steps of oxidation from vitamin A (retinol), plays an indispensible role in the development of the kidney and many other organs. Enzymes that catalyze the oxidation of retinol to generate atRA, including aldehyde dehydrogenase 1 family (ALDH1)A1, ALDH1A2 and ALDH1A3, exhibit complex expression patterns at different stages of renal development. However, molecular triggers that control these differential expression levels are poorly understood. In this study, we provide in vitro evidence to demonstrate that Wilms' tumor 1 (WT1) negatively regulates the expression of the atRA synthetic enzymes, ALDH1A1, ALDH1A2 and ALDH1A3, in the 293 cell line, leading to significant blockage of atRA production. Furthermore, we demonstrate that the suppression of ALDH1A1 by WT1 can be markedly attenuated by histone deacetylase inhibitors (HDACis). Taken together, we provide evidence to indicate that WT1 and HDACs are strong regulators of endogenous retinoic acid synthetic enzymes in 293 cells, indicating that they may be involved in the regulation of atRA synthesis.

show abstract

“…Procedures of extracting retinoid from liver, plasma, and serum were carried out in dark room under red light to avoid retinoid degradation. Retinol and retinoic acid in liver and serum were analyzed according to previously reported method (Yang and others 2005). The linear range of retinol was 0.25 to 8 μg/mL ( r > 0.999) for the retinol in liver homogenate and 0.05 to –1 μg/mL ( r > 0.999) for the retinol in serum.…”

Section: Methodsmentioning

confidence: 99%

Increased Exposure of Vitamin A by Chrysanthemum morifolium Ramat Extract in Rat Was Not via Induction of CYP1A1, CYP1A2, and CYP2B1

Wang

Pan

Chen

et al. 2012

Journal of Food Science

View full text Add to dashboard Cite

The aim of this study was to investigate the effect of Chrysanthemum morifolium Ramat (CM) extract on the pharmacokinetics of retinol and activities of cytochrome P450s (CYP450s) related to retinoid metabolism. Rats were treated with CM extract for 15 d. Plasma concentrations of retinol were measured following oral administration of retinol (45 mg/kg). Basal levels of retinol and retinoic acid in serum and liver were also measured. 7-Ethoxyresorufin-O-deethylase activity, phenacetin-O-deethylase activity, and 7-pentoxyresorufin-O-deethylase activities were used to assay the activities of CYP1A1, CYP1A2, and CYP2B1 in hepatic microsomes of rats, respectively. Protein expressions of the 3 CYP450s were measured by western blot. Our studies demonstrated that CM extract dose-dependently increased basal level of retinol in serum. In pharmacokinetic experiment, CM extract dose-dependently increased plasma concentrations of retinol after oral administration of retinol to rats treated with CM extract. But activities and expressions of CYP1A1, CYP1A2, and CYP2B1 in hepatic microsomes of rats were also induced by CM extract.

show abstract

Effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin on vitamin A metabolism in mice

Cited by 13 publications

References 28 publications

Human Aldehyde Oxidase 1 Interacts with ATP-binding Cassette Transporter-1 and Modulates its Activity in Hepatocytes

Human Aldehyde Oxidase 1 Interacts with ATP-binding Cassette Transporter-1 and Modulates its Activity in Hepatocytes

Regulation of retinoic acid synthetic enzymes by WT1 and HDAC inhibitors in 293 cells

Increased Exposure of Vitamin A by Chrysanthemum morifolium Ramat Extract in Rat Was Not via Induction of CYP1A1, CYP1A2, and CYP2B1

Contact Info

Product

Resources

About