Effects of 17β-Estradiol on growth-related genes expression in female and male spotted scat (Scatophagus argus)

Ru, Xiaoying; Shi, Hongjuan; Wang, Tuo; Liu, Qian-Qing; Jiang, Dongneng; Peng, Yuhao; Huang, Yang; Zhu, Chunhua; Li, Shui-Sheng; Dong, Ran; Li, Guangli

doi:10.1016/j.cbpb.2020.110492

Cited by 12 publications

(9 citation statements)

References 78 publications

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“…Furthermore, the synthesis and secretion of the type 1 insulin-like growth factor in the liver will be promoted once the gh protein binds to the ghr . The type 1 insulin-like growth factor reaches to the body tissues through blood circulation and promotes the proliferation and differentiation of cells and growth and development of individuals. − Several studies reported that high expression of genes of GH/IGF axis was associated with the more rapid growth of fishes, and the greater abundance of gh mRNA in pituitary could result in faster growth in fishes. − The recent two studies reported that in fish, the abundance of gh mRNA in the female pituitary was higher than that of the male, so the faster growth rate was observed in females. , In zebrafish, it was found that TDCIPP could downregulate the genes expression of GH/IGF axis and inhibit growth, although the expression of some genes were measured in the whole brain. , …”

Section: Resultsmentioning

confidence: 99%

“…30−33 The recent two studies reported that in fish, the abundance of gh mRNA in the female pituitary was higher than that of the male, so the faster growth rate was observed in females. 34,35 In zebrafish, it was found that TDCIPP could downregulate the genes expression of GH/IGF axis and inhibit growth, although the expression of some genes were measured in the whole brain. 24,27 In this study, after exposure to 5 or 50 μg/ L TDCIPP for 90 days, the expressions of gh1 and gh2 in the pituitary were significantly downregulated (Figure 2B).…”

Section: Treatment With Tdcipp Inhibited the Expression Of Genesmentioning

confidence: 99%

“…It was reported that the growth of fish was regulated by the GH/IGF axis, , and greater expressions of genes involved in GH/IGF axis (e.g., gh ) could result in more rapid growth. − Previous studies have reported that in the GH/IGF axis of fish, growth hormone releasing hormone (GHRH) is actively expressed in the hypothalamus, whereas the growth hormone releasing hormone receptor (Ghrhr) and growth hormone ( gh ) are highly expressed in the pituitary. , Therefore, distinction and separation of the hypothalamus and pituitary is of great significance to evaluate the effect of TDCIPP on tissue-specific expression of GH/IGF axis genes in fish. However, due to the small size of zebrafish, it was rather difficult to dissect the hypothalamus and pituitary gland from the whole brain tissue, and thus the effects of TDCIPP on the expression of genes of GH/IGF axis was evaluated in the whole brain tissue in our previous study .…”

Section: Introductionmentioning

confidence: 99%

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Tris(1,3-dichloro-2-propyl)phosphate Reduces Growth Hormone Expression via Binding to Growth Hormone Releasing Hormone Receptors and Inhibits the Growth of Crucian Carp

Zhang

Huang

et al. 2021

Environ. Sci. Technol.

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Tris(1,3-dichloro-2-propyl)phosphate (TDCIPP) has commonly been used as an additive flame retardant and frequently detected in the aquatic environment and in biological samples worldwide. Recently, it was found that exposure to TDCIPP inhibited the growth of zebrafish, but the relevant molecular mechanisms remained unclear. In this study, 5 day-old crucian carp (Carassius auratus) larvae were treated with 0.5, 5, or 50 μg/L TDCIPP for 90 days; the effect on growth was evaluated; and related molecular mechanisms were explored. Results demonstrated that 5 or 50 μg/L TDCIPP treatment significantly inhibited the growth of crucian carp and downregulated the expression of growth hormones (ghs), growth hormone receptor (ghr), and insulin-like growth factor 1 (igf1). Molecular docking, dual-luciferase reporter gene assay, and in vitro experiments demonstrated that TDCIPP could bind to the growth hormone releasing hormone receptor protein of crucian carp and disturb the stimulation of growth hormone releasing hormone to the expression of ghs, resulting in the decrease of the mRNA level of gh1 and gh2 in pituitary cells. Our findings provide new perceptions into the molecular mechanisms of developmental toxicity of TDCIPP in fish.

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Section: Resultsmentioning

confidence: 99%

Section: Treatment With Tdcipp Inhibited the Expression Of Genesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Tris(1,3-dichloro-2-propyl)phosphate Reduces Growth Hormone Expression via Binding to Growth Hormone Releasing Hormone Receptors and Inhibits the Growth of Crucian Carp

Zhang

Huang

et al. 2021

Environ. Sci. Technol.

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“…The expression pattern of differentially expressed genes (DEGs) in RNA-seq analysis was verified by RT-qPCR ( 34 ). Total RNA was extracted from liver tissues of the control group and sbGnRH peptides treated group using Trizol according to manufacturer’s instructions (Invitrogen, CA, USA).…”

Section: Methodsmentioning

confidence: 99%

Physiological Effects and Transcriptomic Analysis of sbGnRH on the Liver in Pompano (Trachinotus ovatus)

Xilin

Liu²,

Ndandala

et al. 2022

Front. Endocrinol.

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Pompano (Trachinotus ovatus) is one of the important economic marine fishes in the south coast of China. At present, the research on the basic biology of pompano is relatively weak, which has seriously affected the development of this economic important fish. The liver is an important digestive and metabolic organ of fish which plays an important regulatory role in its growth and development. It is necessary to clarify the effects of sea bream gonadotropin releasing hormone (sbGnRH) on liver physiology and metabolic enzyme activity. The effects of sbGnRH peptides (10 ng/gbw) on the physiological and biochemical indices and metabolic enzyme activities of pompano liver were studied. It was found that after injection of 10 ng/gbw sbGnRH peptides, the contents of albumin, high-density lipoprotein cholesterol, low-density lipoprotein cholesterol, glucose, creatine kinase, iron, magnesium, aspartate aminotransferase, alanine aminotransferase and creatinine increased, while of cholesterol and calcium contents decreased. The activities of amylase, lipase, pyruvate kinase, acyl CoA oxidase, superoxide dismutase, phospholipid hydroperoxide glutathione peroxidase, catalase, glucose-6-phosphate dehydrogenase, fatty acid synthase and lipoprotein lipase increased, while the activities of malic enzyme, carnitine acyl, carnitine translocation, acetyl CoA carboxylase and malondialdehyde decreased. Three hours after the injection of different concentrations of sbGnRH peptides (0 and 10 ng/gbw), the transcriptome sequences of the two groups of livers were sequenced. After quality control and removal of some low-quality data, clean reads of 21,283,647、19,427,359、21,873,990、21,732,174、23,660,062 and 21,592,338 were obtained respectively. In this study, 99 genes were screened and identified as differentially expressed genes, including 77 up-regulated genes and 22 down-regulated genes. According to the Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Ontology (GO) pathway analyses, these pathways and the typical genes involved can be divided into cellular processes, environmental information processing, genetic information processing, diseases, metabolism and organismal systems. The results from this study provide a the oretical basis for studying the effects of sbGnRH on the physiology, biochemistry and metabolic enzyme activities of liver in pompano.

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“…S. argus is also known to exhibit a typical SSD at commercial size under either cultural or natural conditions [ 11 , 30 ], making it an excellent candidate model to elucidate the mechanisms of sexually dimorphic growth. Our previous studies have revealed the sex-biased expressions of ghrh , gh , and igf1 as well as the influences of exogenous estradiol-17β (E 2 ) on them [ 8 , 11 , 16 , 31 ], but no report on the regulatory roles of liver tissue is available and the exact physiologic and molecular mechanisms responsible for SSD is still far from clear. In this study, the differences of liver physiological indicators between male and female S. argus were examined and a comparative transcriptome analysis was further performed to characterize the responsive genes in the liver, aiming to provide a global view of the sexual differences in hepatic biological processes and to explore the potential pathways responsible for inducing SSD.…”

Section: Introductionmentioning

confidence: 99%

Comparative Physiological and Transcriptomic Profiling Offers Insight into the Sexual Dimorphism of Hepatic Metabolism in Size-Dimorphic Spotted Scat (Scatophagus argus)

Chen

Jiang

et al. 2021

Life

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The spotted scat (Scatophagus argus) is an economically important cultured marine fish that exhibits a typical sexual size dimorphism (SSD). SSD has captivated considerable curiosity for farmed fish production; however, up till now the exact underlying mechanism remains largely unclear. As an important digestive and metabolic organ, the liver plays key roles in the regulation of fish growth. It is necessary to elucidate its significance as a downstream component of the hypothalamic-pituitary-liver axis in the formation of SSD. In this study, the liver physiological differences between the sexes were evaluated in S. argus, and the activity of several digestive and metabolic enzymes were affected by sex. Females had higher amylase, protease, and glucose-6-phosphate dehydrogenase activities, while males exhibited markedly higher hepatic lipase and antioxidant enzymes activities. A comparative transcriptomics was then performed to characterize the responsive genes. Illumina sequencing generated 272.6 million clean reads, which were assembled into 79,115 unigenes. A total of 259 differentially expressed genes were identified and a few growth-controlling genes such as igf1 and igfbp1 exhibited female-biased expression. Further analyses showed that several GO terms and pathways associated with metabolic process, particularly lipid and energy metabolisms, were significantly enriched. The male liver showed a more active mitochondrial energy metabolism, implicating an increased energy expenditure associated with reproduction. Collectively, the female-biased growth dimorphism of S. argus may be partially attributed to sexually dimorphic metabolism in the liver. These findings would facilitate further understanding of the nature of SSD in teleost fish.

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Effects of 17β-Estradiol on growth-related genes expression in female and male spotted scat (Scatophagus argus)

Cited by 12 publications

References 78 publications

Tris(1,3-dichloro-2-propyl)phosphate Reduces Growth Hormone Expression via Binding to Growth Hormone Releasing Hormone Receptors and Inhibits the Growth of Crucian Carp

Tris(1,3-dichloro-2-propyl)phosphate Reduces Growth Hormone Expression via Binding to Growth Hormone Releasing Hormone Receptors and Inhibits the Growth of Crucian Carp

Physiological Effects and Transcriptomic Analysis of sbGnRH on the Liver in Pompano (Trachinotus ovatus)

Comparative Physiological and Transcriptomic Profiling Offers Insight into the Sexual Dimorphism of Hepatic Metabolism in Size-Dimorphic Spotted Scat (Scatophagus argus)

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