Effects of 17alpha-methyltestosterone on seminal vesicle development and semen release response in the African catfish, Clarias gariepinus

Viveiros, Ana Tereza de Mendonça; Eding, E.H.; Komen, J.

doi:10.1530/rep.0.1220817

Cited by 21 publications

(7 citation statements)

References 11 publications

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“…African catfish Clarias gariepinus has a putative XX/XY genetic sex determination system (Viveiros et al., ) that can be overridden by high temperature (Santi et al., ). In this species, the thermosensitive period of sex differentiation extends from 0 to 23 days posthatching (dph) with a maximal sensitivity from 6 to 8 dph.…”

Section: Introductionmentioning

confidence: 99%

Temperature Preference and Sex Differentiation in African Catfish, Clarias gariepinus

et al. 2017

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The African catfish Clarias gariepinus has a genetic sex determination system in which high temperature induces masculinization. The thermosensitive period for sex differentiation is short and occurs very early (from 6 to 8 days posthatching [dph]). As young juveniles can encounter high masculinizing temperature (36.5°C) in African water points, we aimed to determine the thermal preference of sexually undifferentiated juveniles and investigate if they spontaneously move toward high masculinizing temperature. Experiments were carried out in an environmental continuum (28-28-28°C and 28-32-36.5°C) made up of three 50-L aquariums connected together. Four hundred larvae from 10 different full-sib progenies were reared successively from 2 to 14 dph in these facilities. Before and after thermal treatments, fish were reared at 28°C until sex ratio determination at 70 dph. In the control continuum, fish were nearly equally distributed in the three compartments. Conversely, in the thermal continuum, compartment occupation significantly differed with progeny and period. During the highly thermosensitive period, two of five progenies significantly preferred (54.7% and 39.8% occupation) the 36.5°C compartment. All tested progenies reared in thermal continuum and separated 36.5°C aquarium showed a skewed sex ratio toward the male phenotype (78-100%). Nevertheless, no correlation was found between 36.5°C compartment occupation and sex ratio in thermal continuum groups. As masculinization temperature could be encountered in African water points during the spawning season, we discussed the adaptive advantage for the African catfish to display a sex differentiation process controlled by a temperature effect.

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Section: Introductionmentioning

confidence: 99%

Temperature Preference and Sex Differentiation in African Catfish, Clarias gariepinus

et al. 2017

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“…Motility was evaluated by subjectively assessing movement of sperm cells under the microscope (Viveiros et al, 2001;Fauvel et al, 2010). A drop of semen was put on a warm slide plus warm distilled water.…”

Section: Milt Collection and Analysismentioning

confidence: 99%

Effect of dietary vernonia amygdalina del. supplementation on some biological parameters of milt of male African catfish (Clarias gariepinus)

Ajala¹,

Owoyemi²

2016

BJVM

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The effect of Vernonia amygdalina Del. leaves on the reproductive potential of male Clarias gariepinus was investigated using 56 fish (425±75.7 g). Four diets with crude protein level of 45% were formulated with different inclusion levels of V. amygdalina leaves. The leaves were added to the basal diet at 0% (control), 5%, 10% and 15% inclusion levels. The 56 male C. gariepinus were randomly distributed in duplicate into 4 concrete ponds and 4 plastic tanks at a stocking density of 7 fish per concrete pond and plastic tank. The fish were fed at 3% body weight twice daily for 45 days. The milt was then analysed for some biological parameters. Motility and liveability percentage values (88.33±14.720% and 91.83±6.824% respectively) were the lowest in the control (0%) group but the difference was not significant (P>0.05) across the groups. Morphology studies showed that total sperm abnormalities were the lowest in the 10% group (45.67±2.73) and total normal sperm cell number was the highest in the 10% group (393.83±14.15). Total sperm counted in morphology study was the highest in controls (442.50±22.12). The differences in these values were not significant across the groups (P>0.05). The results of the study show that studied inclusion levels of V. amygdalina did not have significant effects on sperm motility, liveability and morphology; hence it can be concluded that Bitter leaf may be included at the studied levels with no untoward effect in the diet of male C. gariepinus.

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“…The success of catfish fingerling mass production involves the contribution of quality male broodstocks in spawning activities. The abundance of the sperm production and sperm motility is one of the important factors in spawning success [1]. One pair of African catfish (Clarias gariepinus) can produce 50,000 to 60,000 catfish larvae and further rearing was only obtained 32,500-38,000 juveniles catfish aged 5-6 weeks (mortality ranges from 35-45%) (Steyn and van Vuren, 2011).…”

Section: Introductionmentioning

confidence: 99%

Variation Amount of Sperm Cells in the First Generation of Nontransgenic and Transgenic Mutiara Catfish Hybrid

Buwono

Andriani

Lili

et al. 2019

AJB2T

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One of the factors that influence the success of catfish spawning, including male broodstock gonad fertility determine differences in the number of the sperm produced. The use of transgenesis technology (insertion of the exogenous growth hormone gene) in catfish shows an influence especially multiplication of the sperm counts. The purpose of this study was to compare the variations in the number of sperm cells of the male transgenic Mutiara catfish (comprising the African catfish growth hormone gene) with nontransgenic Mutiara. These research was conducted at the Fish Hatchery, Fisheries Biotechnology Laboratory, and Central Laboratory of Padjadjaran University. The experimental method was used completely randomized design and analyzed quantitatively and qualitatively. The male catfish broodstock were used as treatments consists of the first generation transgenic Mutiara hybrids with nontransgenic Mutiara (G1_MT-MNT), transgenic Mutiara hybrids with Sangkuriang (G1_MTS), and Sangkuriang (G1_S). The sperm from testes of each broodstock were used as replications for sperm count test parameters and motility (n = 5). Qualitative analysis was used for the identification parameters of the transgenic male broodstock using the Polymerase Chain Reaction (PCR) and sperm volume. Quantitative analysis uses the Sigma Plot 12.0 program for parameters for sperm cell count and sperm motility. The results showed that the highest number of sperm (6.06 x 106 cells) and the highest motility (score 4.2) were found in the broodstock of the G1_MT-MNT male catfish.

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Effects of 17alpha-methyltestosterone on seminal vesicle development and semen release response in the African catfish, Clarias gariepinus

Cited by 21 publications

References 11 publications

Temperature Preference and Sex Differentiation in African Catfish, Clarias gariepinus

Temperature Preference and Sex Differentiation in African Catfish, Clarias gariepinus

Effect of dietary vernonia amygdalina del. supplementation on some biological parameters of milt of male African catfish (Clarias gariepinus)

Variation Amount of Sperm Cells in the First Generation of Nontransgenic and Transgenic Mutiara Catfish Hybrid

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