2003
DOI: 10.1128/aac.47.9.2984-2986.2003
|View full text |Cite
|
Sign up to set email alerts
|

Effects of 16S rRNA Gene Mutations on Tetracycline Resistance in Helicobacter pylori

Abstract: The triple-base-pair 16S rDNA mutation AGA 926-928 3TTC mediates high-level tetracycline resistance in Helicobacter pylori. In contrast, single-and double-base-pair mutations mediated only low-level tetracycline resistance and decreased growth rates in the presence of tetracycline, explaining the preference for the TTC mutation in tetracycline-resistant H. pylori isolates.Tetracycline is a cheap and effective antibiotic for the treatment of Helicobacter pylori infections (7,8), but in the past few years the in… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
1
1

Citation Types

1
42
0
3

Year Published

2004
2004
2017
2017

Publication Types

Select...
6
3

Relationship

1
8

Authors

Journals

citations
Cited by 62 publications
(46 citation statements)
references
References 13 publications
1
42
0
3
Order By: Relevance
“…The incidence of tetracycline-resistant H. pylori has increased recently (12,13,15,16,21,25). All high-level resistant strains have triple nucleotide substitutions at the same positions (965 to 967) in helix 31 of the 16S rRNA, which forms a part of primary tetracycline-binding sites within the 30S subunit.…”
Section: Discussionmentioning
confidence: 99%
“…The incidence of tetracycline-resistant H. pylori has increased recently (12,13,15,16,21,25). All high-level resistant strains have triple nucleotide substitutions at the same positions (965 to 967) in helix 31 of the 16S rRNA, which forms a part of primary tetracycline-binding sites within the 30S subunit.…”
Section: Discussionmentioning
confidence: 99%
“…Recently, other substitution mutations of this triple-nucleotide at the same position in the 16S-rRNA have been detected: AGA → GTA or GGC; and AGA → GGA or AGC as the examples of double or single base change, respectively. However, these single and double substitutions mediated only low-levels of tetracycline resistance (Dailidiene et al, 2002;Gerrits et al, 2003).…”
Section: Mutationsmentioning
confidence: 99%
“…The resistance of H. pylori to tetracyclines is reported to be caused by mutations in the 16S rRNA. H. pylori isolates exhibiting AGA 926-928 3 TTC triple-base-pair mutations (4,5,22) show MICs higher than 4 mg/liter and probably represent the clinically most relevant strains, whereas single-or double-base-pair mutations were, rather, associated with MICs between 1 mg/liter and 4 mg/liter (3, 5).The susceptibility of H. pylori to tetracycline is routinely examined by agar diffusion (Etest) or agar dilution tests, which are accepted to be the "gold standards" (10). These methods are slow and time-consuming, and they sometimes fail due to a lack of growth of the infecting H. pylori strain or due to overgrowth with contaminating bacteria.…”
mentioning
confidence: 99%