Effects of 10 days of modest intermittent hypoxia on circulating measures of inflammation in healthy humans

Querido, Jordan S.; Sheel, A. William; Cheema, Rupi; Eeden, Stephan Van; Mulgrew, Alan; Ayas, Najib

doi:10.1007/s11325-011-0555-4

Cited by 27 publications

(16 citation statements)

References 34 publications

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“…We verified O 2 transitions in media using direct real-time measurements; however, the ultimate question is whether such changes in O 2 availability induce any biological responses in cells and if so, what O 2 level and duration of hypoxia is required. Similar to others, we documented both HIF and NF-κB activation after IH exposure (Nanduri et al 2008;Oliver et al 2009;Prabhakar et al 2010;Yuan et al 2011;Quintero et al 2013;Wang et al 2013a), confirming thus possible molecular mechanisms for IH-related pro-inflammatory effects (Querido et al 2012;Baessler et al 2013;Murase et al 2013;He et al 2014). Furthermore, the severity of exposure required for NF-κB activation was of similar intensity to that required for HIF activation (cycling between 4% and 1% O 2 ), potentially suggesting shared intracellular pathways and mutual interactions between these two transcription factors, as suggested previously (Jung et al 2003;Scholz et al 2013).…”

supporting

confidence: 90%

System for exposing cultured cells to intermittent hypoxia utilizing gas permeable cultureware

Polàk¹,

Studer-Rabeler²,

McHugh³

et al. 2015

gpb

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Tissue intermittent hypoxia (IH) occurs in obstructive sleep apnea, sickle cell anemia, physical exercise and other conditions. Poor gas solubility and slow diffusion through culture media hampers mimicking IH-induced transitions of O(2) in vitro. We aimed to develop a system enabling exposure of cultured cells to IH and to validate such exposure by real-time O(2) measurements and cellular responses. Standard 24-well culture plates and plates with bottoms made from a gas permeable film were placed in a heated cabinet. Desired cycling of O(2) levels was induced using programmable solenoids to purge mixtures of 95% N(2) + 5% CO(2) or 95% O(2) + 5% CO(2). Dissolved oxygen, gas pressure, temperature, and water evaporation were measured during cycling. IH-induced cellular effects were evaluated by hypoxia inducible factor (HIF) and NF-κB luciferase reporters in HEK296 cells and by insulin secretion in rat insulinoma cells. Oxygen cycling in the cabinet was translated into identical changes of O(2) at the well bottom in gas permeable, but not in standard cultureware. Twenty-four hours of IH exposure increased HIF (112%), NF-κB (111%) and insulin secretion (44%). Described system enables reproducible and prolonged IH exposure in cultured cells while controlling for important environmental factors.

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supporting

confidence: 90%

System for exposing cultured cells to intermittent hypoxia utilizing gas permeable cultureware

Polàk¹,

Studer-Rabeler²,

McHugh³

et al. 2015

gpb

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“…transcription factor nuclear factor-kappa β). In contrast to the results of intermittent hypoxia in mice and rats, nocturnal exposure to intermittent hypoxia in healthy humans for 10 and 14 days did not increase markers of systemic inflammation 40,41 .…”

Section: Animal and Human Intermittent Hypoxia Experimentscontrasting

confidence: 95%

The Case of Sleep Apnoea and Cardiovascular Events

McEvoy¹

2021

BRN

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For 30 years evidence has pointed to sleep apnoea being a cause of cardiovascular (CV) disease and major CV events. However, five recent large randomised controlled trials (RCTs) of positive airways pressure treatment of sleep apnoea in high CV risk populations have reported neutral results, and in one case, harm. I review the various strands of evidence in an attempt to reconcile these conflicting results arguing that: 1) Cohort and clinical case control studies have likely overestimated the CV risk associated with sleep apnoea; 2) Rodent models have similarly overstated the risk of CV injury from sleep apnoea by focussing on the effects of extremely severe intermittent hypoxia; and 3) Significant heterogeneity in susceptibility to vascular injury may exist between patients. These factors, coupled with low adherence to positive airway pressure mask treatment, may help explain the neutral results of recent RCTs. Suggestions for future research are provided.

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“…Querido et al (29) reported that intermittent hypoxia as a result of airway obstruction would not be responsible for systemic inflammation, which may support that increased systemic inflammation in OSA patients may be more closely associated with other factors. In our study, we found old age, male gender, current smoking status and high AHI level as risk factors of periodontitis.…”

Section: Discussionmentioning

confidence: 94%

The association between periodontitis and obstructive sleep apnea: a preliminary study

Seo

et al. 2012

J of Periodontal Research

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Effects of 10 days of modest intermittent hypoxia on circulating measures of inflammation in healthy humans

Cited by 27 publications

References 34 publications

System for exposing cultured cells to intermittent hypoxia utilizing gas permeable cultureware

System for exposing cultured cells to intermittent hypoxia utilizing gas permeable cultureware

The Case of Sleep Apnoea and Cardiovascular Events

The association between periodontitis and obstructive sleep apnea: a preliminary study

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