Effects of 1-methylcyclopropene (1-MCP) on the expression of genes involved in the chlorophyll degradation pathway of apple fruit during storage

Lv, Jingyi; Zhang, Mengyuan; Bai, Lin; Han, Xuzhou; Ge, Yonghong; Wang, Wenhui; Lǐ, Jiànróng

doi:10.1016/j.foodchem.2019.125707

Cited by 52 publications

(35 citation statements)

References 36 publications

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“…Our results support this notion; the expression levels of SGR2 showed significant changes, but the expression levels of SGR1 were low. The phytohormone ethylene and jasmonic acid could affect the chlorophyll degradation pathway; they could increase the expression of Chl catabolic genes ( Fang et al, 2020 ; Lv et al, 2020 ). Compared to the green pod mutant, the expression levels of chlorophyll degradation genes in the wild type was earlier and higher.…”

Section: Discussionmentioning

confidence: 99%

Enhanced Chlorophyll Degradation Triggers the Pod Degreening of “Golden Hook,” a Special Ecotype in Common Bean (Phaseolus vulgaris L.)

Zhu

et al. 2020

Front. Genet.

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To reveal genetic factors or pathways involved in the pod degreening, we performed transcriptome and metabolome analyses using a yellow pod cultivar of the common bean “golden hook” ecotype and its green pod mutants yielded via gamma radiation. Transcriptional profiling showed that expression levels of red chlorophyll catabolite reductase ( RCCR , Phvul.008G280300 ) involved in chlorophyll degradation was strongly enhanced at an early stage (2 cm long) in wild type but not in green pod mutants. The expression levels of genes involved in cellulose synthesis was inhibited by the pod degreening. Metabolomic profiling showed that the content of most flavonoid, flavones, and isoflavonoid was decreased during pod development, but the content of afzelechin, taxifolin, dihydrokaempferol, and cyanidin 3- O -rutinoside was remarkably increased in both wild type and green pod mutant. This study revealed that the pod degreening of the golden hook resulting from chlorophyll degradation could trigger changes in cellulose and flavonoids biosynthesis pathway, offering this cultivar a special color appearance and good flavor.

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Section: Discussionmentioning

confidence: 99%

Enhanced Chlorophyll Degradation Triggers the Pod Degreening of “Golden Hook,” a Special Ecotype in Common Bean (Phaseolus vulgaris L.)

Zhu

et al. 2020

Front. Genet.

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“…The Chl content in apple peels was measured as previously described (Lv et al., 2020), and ethylene production was measured as described by Tan et al. (2013).…”

Section: Methodsmentioning

confidence: 99%

“…Chl degradation in apple is also known to be regulated by the hormone ethylene. Specifically, ethylene treatment promotes the expression of Chl catabolic genes ( CCG ), which will lead to accelerated Chl degradation during storage, while treatment with the ethylene perception inhibitor 1‐methylcyclopropene (1‐MCP) has the opposite effect (Lv et al., 2020). Ethylene‐induced Chl degradation has been shown to be regulated transcriptionally.…”

Section: Introductionmentioning

confidence: 99%

Ethylene‐activated MdPUB24 mediates ubiquitination of MdBEL7 to promote chlorophyll degradation in apple fruit

Wei

Jin

et al. 2021

The Plant Journal

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SUMMARY Chlorophyll (Chl) degradation is a natural phenomenon that occurs during ripening in many fleshy fruit species, and also during fruit storage. The plant hormone ethylene is a key factor in promoting Chl degradation during fruit storage, but the mechanisms involved in this induction are largely unknown. In this study, an apple (Malus domestica) BEL1‐LIKE HOMEODOMAIN transcription factor 7 (MdBEL7), potentially functioning as a transcriptional repressor of the Chl catabolic genes (CCGs), including MdCLH, MdPPH2 and MdRCCR2, was identified as a partner of the ethylene‐activated U‐box type E3 ubiquitin ligase MdPUB24 in a yeast library screen. Yeast‐two‐hybrid, co‐immunoprecipitation and luciferase complementation imaging assays were then used to verify the interaction between MdBEL7 and MdPUB24. In vitro and in vivo ubiquitination experiments revealed that MdPUB24 functions as an E3 ubiquitin ligase to ubiquitinate MdBEL7, thereby causing its degradation through the 26S proteasome pathway. Transient overexpression of MdPUB24 in apple fruit led to a decrease in MdBEL7 abundance and increased expression of CCG genes, including MdCLH, MdPPH2 and MdRCCR2, as well as greater Chl degradation. Taken together, the data indicated that an ethylene‐activated U‐box type E3 ubiquitin ligase MdPUB24 directly interacts with and ubiquitinates MdBEL7. Consequent degradation of MdBEL7 results in enhanced expression of MdCLH, MdPPH2 and MdRCCR2, and thus Chl degradation during apple fruit storage. Our results reveal that an ethylene‐MdPUB24‐MdBEL7 module regulates Chl degradation by post‐translational modification during apple fruit storage.

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“…The method of applying chemical or physical preservatives is widely employed to conserve fresh fruit because this method is the simplest and least expensive [ 3 ]. For climacteric fruit, the ethylene production rate usually increases rapidly during storage, which may accelerate the process of fruit softening or lead to physiological disorders [ 10 ]. Previous studies reported that the postharvest treatment with 1-methylcyclopropene (1-MCP) can effectively delay fruit ripening and softening processes and prolong the storage life of various fruits [ 11 , 12 ] because it can bind to ethylene receptors in plant cells and inhibit ethylene action of fruit by suppressing ethylene biosynthesis-related genes expression levels [ 13 , 14 ].…”

Section: Introductionmentioning

confidence: 99%

Transcriptome profiling helps to elucidate the mechanisms of ripening and epidermal senescence in passion fruit (Passiflora edulia Sims)

Xin

et al. 2020

PLoS ONE

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Passion fruit (Passiflora edulia Sims), an important tropical and subtropical species, is classified as a respiration climacteric fruit, and its quality deteriorates rapidly after harvest. To elucidate the mechanisms involved in ripening and rapid fruit senescence, phytochemical characteristic analysis and RNA sequencing were performed in purple passion fruit with different treatments, that is, 1-methylcyclopropene (1-MCP) and preservative film (PF). Comprehensive functional annotation and KEGG enrichment analysis showed that starch and sucrose metabolism, plant hormone signal transduction, phenylpropanoid biosynthesis, flavonoid biosynthesis, and carotenoid biosynthesis were involved in fruit ripening. Treatment with PF and 1-MCP significantly affected the transcription levels of passion fruit during postharvest storage. A large number of differentially expressed unigenes (DEGs) were identified as significantly enriched in starch and sucrose metabolism, plant hormone signal transduction and phenylpropanoid biosynthesis at the postharvest stage. The PF and 1-MCP treatments increased superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD) gene expression levels and enzyme activities, accelerated lignin accumulation, and decreased βgalactosidase (β-Gal), polygalacturonase (PG) and cellulose activities and gene expression levels to delay cell wall degradation during fruit senescence. The RNA sequencing data for cell wall metabolism and hormone signal transduction pathway-related unigenes were verified by RT-qPCR. The results of this study indicate that the cell wall metabolism and hormone signaling pathways are closely related to passion fruit ripening. PF and 1-MCP treatment might inhibit ethylene signaling and regulate cell wall metabolism pathways to inhibit cell wall degradation. Our results demonstrate the involvement of ripening-and senescence-related networks in passion fruit ripening and may establish a foundation for future research investigating the effects of PF and 1-MCP treatment on fruit ripening.

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Effects of 1-methylcyclopropene (1-MCP) on the expression of genes involved in the chlorophyll degradation pathway of apple fruit during storage

Cited by 52 publications

References 36 publications

Enhanced Chlorophyll Degradation Triggers the Pod Degreening of “Golden Hook,” a Special Ecotype in Common Bean (Phaseolus vulgaris L.)

Enhanced Chlorophyll Degradation Triggers the Pod Degreening of “Golden Hook,” a Special Ecotype in Common Bean (Phaseolus vulgaris L.)

Ethylene‐activated MdPUB24 mediates ubiquitination of MdBEL7 to promote chlorophyll degradation in apple fruit

Transcriptome profiling helps to elucidate the mechanisms of ripening and epidermal senescence in passion fruit (Passiflora edulia Sims)

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