Effects of 1.8GHz radiofrequency field on DNA damage and expression of heat shock protein 70 in human lens epithelial cells

Sun, Limei; Yao, Ke; Wang, Kaijun; Lu, Di; Hu, Huajun; Gao, Xiangwei; Wang, Baohong; Wei, Zheng; Lou, J. L.; Wu, Wei

doi:10.1016/j.mrfmmm.2006.08.010

Cited by 102 publications

(51 citation statements)

References 24 publications

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“…Only 15% of this radiation causing cell death are related to microwaves; the other amount of radiation seems to be due to the frequency of 1.885 GHz. However, the cellular effects as observed here are in accordance with previous studies on other cell types [18,19] and seem to be related to oxidative stress [18][19][20][21][22].…”

Section: Cell Culture and Test Proceduressupporting

confidence: 93%

Neutralization of wireless DECT base radiation by novel resonance devices

Dartsch¹,

König²

2017

Integr Mol Med

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Wireless telecommunication sources working with frequencies ranging from 0.9 to 2.5 GHz are still increasing rapidly. Among these are also digitally enhanced cordless telecommunication (DECT) phones which emit only a weak radiation when an active DECT base and its handset are separated from each other. To address this topic, we investigated the cellular effects of DECT base radiation and its possible compensation by specially designed novel resonance devices. Connective tissue fibroblasts (L-929) were exposed to the radiation of an active commercially available DECT base ± single resonance devices or combination of them. Unexposed cells in another incubator without any DECT base radiation served as corresponding controls. Cell vitality was checked by measurement of the enzymatic activity of mitochondrial dehydrogenases by the color change of the sodium salt 2,3-bis[2-methoxy-4-nitro-5-sulfo-pheny]-2H-tetrazolium-5-carboxyanilide (XTT).The results clearly demonstrate that exposure to DECT base radiation caused a significantly reduced cell vitality which was accompanied by marked morphological changes in the cells such as intracellular vacuolization, rounding and detachment. Approximately 15% of the reducing potential was related to microwave-related warming. Reduction in cell vitality after DECT base radiation exposure was partially neutralized by use of one of the novel resonance devices alone or in combination. Especially resonance device type A-1 was very effective and its potential for neutralization could be increased to 95% by additionally using two crossed resonance devices type RD-B with their plus end directed towards the cells.

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Section: Cell Culture and Test Proceduressupporting

confidence: 93%

Neutralization of wireless DECT base radiation by novel resonance devices

Dartsch¹,

König²

2017

Integr Mol Med

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“…There is also little evidence for indirect DNA effects, including alterations in gene expression, cell proliferation, or apoptosis (Laszlo et al, 2005;Chauhan et al, 2006;Lixia et al, 2006;Qutob et al, 2006;Joubert et al, 2007). Thus, laboratory studies do not support the possibility that mobile phone use increases the risk of brain tumours.…”

Section: Discussionmentioning

confidence: 99%

Mobile phone use, exposure to radiofrequency electromagnetic field, and brain tumour: a case–control study

et al. 2008

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In a case -control study in Japan of brain tumours in relation to mobile phone use, we used a novel approach for estimating the specific absorption rate (SAR) inside the tumour, taking account of spatial relationships between tumour localisation and intracranial radiofrequency distribution. Personal interviews were carried out with 88 patients with glioma, 132 with meningioma, and 102 with pituitary adenoma (322 cases in total), and with 683 individually matched controls. All maximal SAR values were below 0.1 W kg À1 , far lower than the level at which thermal effects may occur, the adjusted odds ratios (ORs) for regular mobile phone users being 1.22 (95% confidence interval (CI): 0.63 -2.37) for glioma and 0.70 (0.42 -1.16) for meningioma. When the maximal SAR value inside the tumour tissue was accounted for in the exposure indices, the overall OR was again not increased and there was no significant trend towards an increasing OR in relation to SAR-derived exposure indices. A non-significant increase in OR among glioma patients in the heavily exposed group may reflect recall bias. British Journal of Cancer (2008) The rapid increase in mobile phone use has raised public concern about their safety (Rothman et

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“…Studies on human lens epithelial cells in exposing to 1.8 GHz at SAR of 3 and 4 W/Kg field showed increased SSB and irreversible DNA damage [9,17]. The exposure of human lens epithelial cells (HLEC) to microwaves of 1800 MHz resulted in repairable DNA damage [9,18]. However, some studies have shown absence of DNA damage in human lymphocyte and leukocyte culture on exposure to RFR emitted from cell phone [19][20][21] and in rat brain exposed to 915 MHz GSM microwaves [22,23].…”

Section: Introductionmentioning

confidence: 96%

“…RF radiation on rabbit lens showed various biochemical changes in the form of altered protein expression on cell membranes of lens epithelial cells, which declined cell proliferation, and lens epithelial cells damage and opacity [6][7][8]. The heat shock proteins, HSP-70 and HSP-27, in lens epithelial cells [9,10] are reported to increase due to radiation exposure. Oxidative stress is a leading cause for cataractogenesis.…”

Section: Introductionmentioning

confidence: 99%

“…Diem et al [16] reported that the intermittent exposure schedule caused significantly more DNA damage than continuous exposure. Studies on human lens epithelial cells in exposing to 1.8 GHz at SAR of 3 and 4 W/Kg field showed increased SSB and irreversible DNA damage [9,17]. The exposure of human lens epithelial cells (HLEC) to microwaves of 1800 MHz resulted in repairable DNA damage [9,18].…”

Section: Introductionmentioning

confidence: 99%

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Effect of Ultrahigh Frequency Radiation Emitted from 2G Cell Phone on Developing Lens of Chick Embryo: A Histological Study

D'Silva

Swer

Anbalagan

et al. 2014

Advances in Anatomy

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A Mobile phone in operation emits a pulsed radiofrequency electromagnetic field which is absorbed into the user's body particularly the head region. Contradictory scientific reports on the health effect of nonionizing radiations on biological tissues have prompted to undertake the present study to evaluate the damage in the developing lens of a chick embryo following exposure to radiation emitted from a 2G cell phone. Fertilized chick embryos were incubated in two groups in a standard egg incubator. The experiment group was exposed to radiation emitted from a 2G cell phone. On completion of scheduled duration, the embryos were collected and processed for routine histological studies. The 9th to 12th day chick embryo eyes were processed for assessment of DNA damage using the alkaline comet assay technique. The lens thickness and the equatorial diameter were measured using oculometer and statistically compared for both groups. In the present study, the exposure of chick embryos to a 2G cell phone caused structural changes in lens epithelial cells, formation of cystic cells and spaces, distortion of lens fibers, and formation of posterior aberrant nuclear layer. The DNA damage in the developing eyes of the experiment group assessed by comet assay was highly significant.

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Effects of 1.8GHz radiofrequency field on DNA damage and expression of heat shock protein 70 in human lens epithelial cells

Cited by 102 publications

References 24 publications

Neutralization of wireless DECT base radiation by novel resonance devices

Neutralization of wireless DECT base radiation by novel resonance devices

Mobile phone use, exposure to radiofrequency electromagnetic field, and brain tumour: a case–control study

Effect of Ultrahigh Frequency Radiation Emitted from 2G Cell Phone on Developing Lens of Chick Embryo: A Histological Study

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