Effects of 1,25-dihydroxyvitamin D&lt;sub&gt;3&lt;/sub&gt; on Macrophage Cytokine Secretion Stimulated by &lt;i&gt;Porphyromonas gingivalis&lt;/i&gt;

Xu, Qingan; Li, Zhaofei; Zhang, Ping; Cao, Li-Hua; Fan, Mingwen

doi:10.7883/yoken.jjid.2015.396

Cited by 12 publications

(12 citation statements)

References 34 publications

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“…Research has also found that patients with low serum vitamin D levels, exhibited healing from periodontal surgery was adversely affected [100]. Related to these clinical findings, vitamin D improves epithelial innate immune functions and antimicrobial peptide secretion [101,102,103] and down-regulates NFκB activation and cytokine secretion by monocytes and macrophages [104,105]. Additionally, from the microbiome side of the periodontal disease equation, vitamin D decreases virulence gene expression by P. gingivalis [104,105].…”

Section: Discussionmentioning

confidence: 99%

Serum Nutrient Levels and Aging Effects on Periodontitis

et al. 2018

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Periodontal disease damages tissues as a result of dysregulated host responses against the chronic bacterial biofilm insult and approximately 50% of US adults >30 years old exhibit periodontitis. The association of five blood nutrients and periodontitis were evaluated due to our previous findings regarding a potential protective effect for these nutrients in periodontal disease derived from the US population sampled as part of the National Health and Nutrition Examination Survey (1999–2004). Data from over 15,000 subjects was analyzed for blood levels of cis-β-carotene, β-cryptoxanthin, folate, vitamin D, and vitamin E, linked with analysis of the presence and severity of periodontitis. Moderate/severe disease patients had lower cis-β-carotene levels across all racial/ethnic groups and these decreased levels in moderate/severe periodontitis were exacerbated with age. β-cryptoxanthin demonstrated lower levels in severe disease patients across the entire age range in all racial/ethnic groups. Folate differences were evident across the various age groups with consistently lower levels in periodontitis patients >30 years and most pronounced in females. Lower levels of vitamin D were consistently noted across the entire age range of patients with a greater difference seen in females with periodontitis. Finally, an analytical approach to identify interactions among these nutrients related to age and periodontitis showed interactions of vitamin D in females, and folate with race in the population. These findings suggest that improving specific nutrient intake leading to elevated blood levels of a combination of these protective factors may provide a novel strategy to affect the significant increase in periodontitis that occurs with aging.

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Section: Discussionmentioning

confidence: 99%

Serum Nutrient Levels and Aging Effects on Periodontitis

et al. 2018

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“…We observed higher levels of TGF-β in vitamin D-treated EAE group. Reducing effects of vitamin D on IL-6 production have been also demonstrated in other studies (41,42). In the presence of the higher levels of TGF-β and lower levels of IL-6 (higher TGF-β/IL-6 ratio), TGF-β leads to differentiation of protective Treg cells (38).…”

Section: Discussionmentioning

confidence: 52%

The Modulatory Effects of Vitamin D on the Expression of IL-12 and TGF-β in the Spinal Cord and Serum of Mice with Experimental Autoimmune Encephalomyelitis

et al. 2018

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KEYWORDS ABSTRACTExperimental Autoimmune Encephalomyelitis Vitamin D IL-12 TGF-β Background & objective:The immunoregulatory effects of transforming growth factor (TGF)-βand interleukin-12 (IL-12) and immunomodulatory actions of vitamin D (VD) were reported in several studies. This study aims to evaluate VD effects on IL-12 and TGF-β expression in experimental autoimmune encephalomyelitis (EAE).Methods: EAE was induced in three groups of C57BL/6 mice by immunization with MOG and administered intra-peritoneally 200 ngVD, PBS or olive oil (OO) from day +3 to +30. One group was also considered as healthy control group. At day 31, cytokines expression in the spinal cord and their serum levels were determined using real time-PCR and ELISA, respectively.Results: IL-12 gene expression and its serum levels in PBS-injected-or OOadministrated EAE groups were significantly higher than healthy group. IL-12 gene expression in EAE group treated with VD was significantly decreased compared to PBS-injected-or OO-administrated EAE groups (P<0.001 and P<0.02, respectively). Serum IL-12 levels were significantly reduced in VD-treated EAE mice compared to PBS-injected-or OO-administrated EAE groups (P<0.004 and P<0.05, respectively). No significant difference was observed between PBS-injected-EAE, OOadministrated EAE and healthy groups regarding the expression of TGF-β. In EAE mice treated with VD, TGF-β expression was significantly higher than healthy group and PBS-injected EAE mice (P<0.01 and P<0.04, respectively). VD-treated EAE group had also higher serum TGF-β levels than PBS-injected-, OO-administrated and healthy groups (P<0.05).Conclusion: VD modulates the expression of IL-12 and TGF-β in spinal cord and serum of EAE mice. Article Info

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“…These results indicate that calcitriol could partially abrogate aberrant host inflammatory response when exposed to gram positive bacteria. The immunomodulatory effect that calcitriol has on PAMPexposed leukocyte IL-10 production has a great deal of intra-and interspecies variability [33][34][35][36]. A previous small pilot study in dogs determined that calcitriol did not increase leukocyte production of IL-10 when exposed to LPS or LTA [11].…”

Section: Discussionmentioning

confidence: 99%

Effects of calcitriol on oxidative burst, phagocytic function, and leukocyte cytokine production in shelter dogs

Jaffey

Bessette

Tao

et al. 2020

Canine Genet Epidemiol

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Background: The active metabolite of vitamin D, calcitriol, has been shown across many different species to augment innate immune responses and dampen aberrant proinflammatory cytokine production. Community acquired infections are common in shelters and consume limited shelter resources, impact adoption rates, and can result in unnecessary euthanasia. Prophylactic oral vitamin D supplementation decreases the incidence and severity of upper and lower respiratory tract infections in humans. Before a clinical trial investigating the clinical benefit of oral vitamin D supplementation in shelter dogs can be pursued, an in vitro study evaluating the immunomodulatory effects of calcitriol in blood from shelter dogs is warranted. Therefore, the objective of this study was to determine if incubation of whole blood obtained from apparently healthy dogs housed in a shelter for ≥7 days with calcitriol would alter granulocyte/monocyte (GM) oxidative burst and phagocytic function as well as pathogen-associated molecular pattern (PAMP)-stimulated leukocyte production of tumor necrosis factor (TNF)-α, and interleukin (IL)-6, and IL-10. Results: Ten dogs housed in a shelter for ≥7 days were enrolled in a prospective cohort study. Whole blood from these dogs was incubated with calcitriol (10 − 7 M) or diluent (control) for 24 h. Subsequent to this incubation, phagocytosis of opsonized-Escherichia coli (E. coli) and E. coli-induced oxidative burst were evaluated via flow cytometry. In addition, leukocyte production of TNF-α, IL-6, and IL-10 were measured using a canine-specific multiplex bead assay. Calcitriol significantly decreased leukocyte TNF-α production (p = 0.009) and increased IL-10 production (p = 0.002). Tumor necrosis factor-α-toIL -10 ratio was significantly decreased with calcitriol (p = 0.017), while IL-6 production as well as GM oxidative burst and phagocytic function were not significantly affected. Conclusions: These data indicate that calcitriol attenuates proinflammatory immune responses without affecting GM oxidative burst or phagocytic function in vitro in whole blood obtained from apparently healthy shelter dogs.

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Effects of 1,25-dihydroxyvitamin D<sub>3</sub> on Macrophage Cytokine Secretion Stimulated by <i>Porphyromonas gingivalis</i>

Cited by 12 publications

References 34 publications

Serum Nutrient Levels and Aging Effects on Periodontitis

Serum Nutrient Levels and Aging Effects on Periodontitis

The Modulatory Effects of Vitamin D on the Expression of IL-12 and TGF-β in the Spinal Cord and Serum of Mice with Experimental Autoimmune Encephalomyelitis

Effects of calcitriol on oxidative burst, phagocytic function, and leukocyte cytokine production in shelter dogs

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