Effects induced by a 50 Hz electromagnetic field and doxorubicin on Walker-256 carcinosarcoma growth and hepatic redox state in rats

Орел, В.Е.; Krotevych, Mykhailo; Dasyukevich, O I; Rykhalskyi, O.; Syvak, L.А.; Tsvir, Helena; Tsvir, Dmytro; Garmanchuk, Lyudmyla; Orel, Valerii В; Sheina, Iryna V.; Rybka, Vladyslava; Shults, Nataliia V.; Suzuki, Yuichiro; Gychka, Sergiy G.

doi:10.1080/15368378.2021.1958342

Cited by 5 publications

(3 citation statements)

References 70 publications

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“…Significant increases in ROS levels have been found to influence the hepatic redox state [256] and were observed in several cell lines just after the end of ELF EMF exposure [213,[257][258][259][260][261][262][263][264][265]. ELF EMF exposure also elevates the expression of RNS and O 2…”

Section: Ros (Such As Superoxide [Omentioning

confidence: 98%

Magnetic Fields and Cancer: Epidemiology, Cellular Biology, and Theranostics

Maffei

2022

IJMS

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Humans are exposed to a complex mix of man-made electric and magnetic fields (MFs) at many different frequencies, at home and at work. Epidemiological studies indicate that there is a positive relationship between residential/domestic and occupational exposure to extremely low frequency electromagnetic fields and some types of cancer, although some other studies indicate no relationship. In this review, after an introduction on the MF definition and a description of natural/anthropogenic sources, the epidemiology of residential/domestic and occupational exposure to MFs and cancer is reviewed, with reference to leukemia, brain, and breast cancer. The in vivo and in vitro effects of MFs on cancer are reviewed considering both human and animal cells, with particular reference to the involvement of reactive oxygen species (ROS). MF application on cancer diagnostic and therapy (theranostic) are also reviewed by describing the use of different magnetic resonance imaging (MRI) applications for the detection of several cancers. Finally, the use of magnetic nanoparticles is described in terms of treatment of cancer by nanomedical applications for the precise delivery of anticancer drugs, nanosurgery by magnetomechanic methods, and selective killing of cancer cells by magnetic hyperthermia. The supplementary tables provide quantitative data and methodologies in epidemiological and cell biology studies. Although scientists do not generally agree that there is a cause-effect relationship between exposure to MF and cancer, MFs might not be the direct cause of cancer but may contribute to produce ROS and generate oxidative stress, which could trigger or enhance the expression of oncogenes.

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Section: Ros (Such As Superoxide [Omentioning

confidence: 98%

Magnetic Fields and Cancer: Epidemiology, Cellular Biology, and Theranostics

Maffei

2022

IJMS

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“…For instance, ROS are well-known to play a critical role in signaling events that initiate apoptosis. The fact that electromagnetic fields modulate ROS levels could point to the potential use of IMH, when locally applied to a tumor, to increase antitumor activity and decrease the toxicity of DOX in other tissues [ 33 , 61 , 62 ]. Programmed cell death poses a barrier that restricts cancer cell survival and dissemination, wherein early apoptosis facilitates phagocytosis by macrophages without releasing pro-inflammatory cellular components, as opposed to necrosis [ 63 , 64 ].…”

Section: Discussionmentioning

confidence: 99%

Combination Treatment with Liposomal Doxorubicin and Inductive Moderate Hyperthermia for Sarcoma Saos-2 Cells

Orel,

Diedkov,

Ostafiichuk

et al. 2024

Pharmaceuticals

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Despite efforts in osteosarcoma (OS) research, the role of inductive moderate hyperthermia (IMH) in delivering and enhancing the antitumor effect of liposomal doxorubicin formulations (LDOX) remains unresolved. This study investigated the effect of a combination treatment with LDOX and IMH on Saos-2 human OS cells. We compared cell viability using a trypan blue assay, apoptosis and reactive oxygen species (ROS) measured by flow cytometry and pro-apoptotic Bax protein expression examined by immunocytochemistry in response to IMH (42 MHz frequency, 15 W power for 30 min), LDOX (0.4 μg/mL), and LDOX plus IMH. The lower IC50 value of LDOX at 72 h indicated increased accumulation of the drug in the OS cells. LDOX plus IMH resulted in a 61% lower cell viability compared to no treatment. Moreover, IMH potentiated the LDOX action on the Saos-2 cells by promoting ROS production at temperatures of <42 °C. There was a 12% increase in cell populations undergoing early apoptosis with a less heterogeneous distribution of Bax after combination treatment compared to those treated with LDOX (p < 0.05). Therefore, we determined that IMH could enhance LDOX delivery and its antitumor effect via altered membrane permeabilization, ROS generation, and a lower level of visualized Bax heterogeneity in the Saos-2 cells, suggesting the potential translation of these findings into in vivo studies.

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“…The ability of iron to stimulate ROS production in cytochrome P450 pathways is clearly important for an increased risk of toxicity (Veith & Moorthy, 2018). EMFs were applied to modulate the hepatic redox state and alleviate DOX‐induced liver toxicity in Walker‐256 carcinosarcoma‐bearing rats (V. E. Orel, Krotevych, et al, 2021). While CMF action alone improved MNCs targeting to the liver, their delivery to the hepatocellular carcinoma cells was affected by rapid uptake in the reticuloendothelial system (Iacobazzi et al, 2022).…”

Section: Limitations and Challenges For Future Clinical Translationmentioning

confidence: 99%

Nanotherapy based on magneto‐mechanochemical modulation of tumor redox state

Orel

Papazoglou

Tsagkaris³

et al. 2022

WIREs Nanomed Nanobiotechnol

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Magnetic nanoparticles (MNs) are typically used as contrast agents for magnetic resonance imaging or as drug carriers with a remotely controlled delivery to the tumor. However, they can also potentiate the action of anticancer drugs under the influence of applied constant magnetic (CMFs) and electromagnetic fields (EMFs). This review demonstrates the role of magneto‐mechanochemical effects produced by MNs alone and loaded with anticancer agents (MNCs) in response to CMFs and EMFs for modulation of tumor redox state. The combined treatment is suggested to act by two mechanisms: spin‐dependent electron transport propagates free radical chain reactions, while magnetomechanical interactions cause conformational changes in drug molecules loaded onto MNs and generate reactive oxygen species (ROS). By adjusting the parameters of CMFs and EMFs during the magneto‐mechanochemical synthesis and subsequent treatment, it is possible to modulate ROS production and switch redox signaling involved in ERK1/2 and NF‐κB pathways from initiation of tumor growth to inhibition. Observations of tumor volume in different animal models and treatment combinations reported a 6%–70% reduction as compared with conventional drugs. Despite these results, there is a general lack of research in magnetic nanotheranostics that link redox changes across multiple levels of organization in the tumor‐bearing host. Further multidisciplinary studies with more focus on the relationship between the electron transport processes in biomolecules and their effects on the tumor‐host interaction should accelerate the clinical translation of magnetic nanotheranostics. This article is categorized under: Therapeutic Approaches and Drug Discovery > Nanomedicine for Oncologic Disease Therapeutic Approaches and Drug Discovery > Emerging Technologies Nanotechnology Approaches to Biology > Nanoscale Systems in Biology

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Effects induced by a 50 Hz electromagnetic field and doxorubicin on Walker-256 carcinosarcoma growth and hepatic redox state in rats

Cited by 5 publications

References 70 publications

Magnetic Fields and Cancer: Epidemiology, Cellular Biology, and Theranostics

Magnetic Fields and Cancer: Epidemiology, Cellular Biology, and Theranostics

Combination Treatment with Liposomal Doxorubicin and Inductive Moderate Hyperthermia for Sarcoma Saos-2 Cells

Nanotherapy based on magneto‐mechanochemical modulation of tumor redox state

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