Effects and possible mechanism of a picosecond pulsed electric field on angiogenesis in cervical cancer in�vitro

Abstract

Picosecond pulsed electric field (psPEF) is an athermal, minimally invasive and local ablative biomedical engineering technique used in cancer therapy. However, to the best of our knowledge, the effect of psPEF on angiogenesis in cervical cancer is unknown. Therefore, the aim of the current study was to investigate the effects and possible mechanism of psPEF on angiogenesis in cervical cancer in vitro. HeLa cell and human umbilical vein endothelial cell (HUVEC) suspensions were exposed to psPEF with an increasing gradient of electric field intensity (0, 200, 400 and 600 kV/cm). A Cell Counting kit-8 assay and flow cytometry were used to investigate the effect of psPEF on the proliferation and apoptosis of HUVECs. The invasion, migration and tube formation capabilities of HUVECs following psPEF treatment were investigated by Transwell invasion assay, scratch test and lumen formation assay, respectively. Changes in the protein and mRNA levels of angiogenesis-associated factors in HeLa cells were detected by western blot analysis and reverse transcription-quantitative polymerase chain reaction. psPEF was identified to inhibit proliferation and tube formation, and induce apoptosis and necrosis of HUVECs in a dose-dependent manner. psPEF was revealed to decrease the protein and mRNA expression levels of vascular endothelial growth factor and hypoxia-inducible factor 1α in HeLa cells. In summary, psPEF exhibited anti-angiogenic effects in cervical cancer in vitro by exerting direct effects on HUVECs and indirect effects on angiogenesis-associated factors in HeLa cells.

The Specificity and Efficacy of Alternating Electric Fields as a Prospective Cancer Treatment

“…The psPEF specification was the 3 Hz frequency, 800 ps duration, 2,000 pulse number, and intensity of 0, 200, 400, and 600 kV/cm in vitro. Moreover, VEGF and HIF1α downregulation in HeLa cells supported the anti-angiogenic effect of psPEF (Wu et al, 2019). Those studies expressed the efficacy of psPEF to inhibit tumour growth in a dose-dependent manner.…”

“…One of the findings is the invention of an electric field device used in cancer treatment, namely TTFields (Kirson et al, 2004). It initiated further research by utilising the electric fields for cancer treatment, and nsPEF (Ren et al, 2013), psPEF (Chen et al, 2013;Wu et al, 2019), and ECCT (Alamsyah et al, 2015) were developed electric fields devices. The last one to mention is the electric field device designed by Dr. Warsito P. Taruno, an Indonesian.…”

Alternating Current-Electric Field Inducing Chorio Allantoic Membrane (CAM) Angiogenesis through Exogenous Growth Factor Intervention

“…Another AC-EF device, picosecond Pulsed electric Fields (psPEF), may block angiogenesis in a cervical cancer xenograft model by downregulating VEGF, HIF1α, and HIF2α [14]. In addition, this device has downregulated VEGF and HIF1α when using HeLa cell culture [15]. However, the influence of intermediate frequency generated by ECCT on angiogenesis remains unexplored.…”