Effects and interactions of prostaglandin F2 alpha, oxytocin, and cytokines on steroidogenesis of porcine luteal cells

Pitzel, L.

doi:10.1210/en.132.2.751

Cited by 39 publications

(16 citation statements)

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“…Dev. 42 : 199-204, 1996) Accepted for publication: June 3, 1996 Correspondence: K. Okuda t is well known that oxytocin (OT) is synthesized and secreted by the corpus luteum (CL) a luteolytic effect [12], or no effect [13,14]. These different results obtained from in vitro studies seem to depend on the experimental model, e.g., shortor long-term culture of luteal cells, cell density and duration of stimulation with reagents.…”

mentioning

confidence: 99%

Progesterone Release of Bovine Corpus Luteum in Response to Oxytocin in Different Culture Systems.

Sakumoto

Ando

Okuda

1996

Journal of Reproduction and Development

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Abstract. The aim of the present study was to investigate the effect of oxytocin (OT) on progesterone secretion by bovine corpus luteum (CL) in different culture systems. When luteal cells were cultured for 1, 2, 4 and 15 h at a density of 6 × 10 4 cells/ml, OT (10 -7 M) did not affect progesterone secretion. On the other hand, bovine LH strongly stimulated progesterone secretion by the cells 2-15 h after incubation (P<0.01). In another experiment, a novel luteal slice culture system was used, in which luteal cells maintained cell-to-cell contact. Three slices (15-20 mg/a slice) from a CL were pre-incubated for 1 h, and then each slice was incubated for 1 h in a culture tube with a medium (3 ml) supplemented with and without bovine LH (100 ng/ml) or OT (10 -7 M). Slices were replaced every 1 h in a fresh medium during the 5 h incubation experiment. A 1-h stimulation with bovine LH and OT induced significant but different effects on the release of progesterone. OT acutely stimulated progesterone release at doses 10 -7 M (155%; P<0.05), and bovine LH significantly increased progesterone release during and after stimulation (223%; P<0.01). These data support the hypothesis that OT plays a role in modulating the mechanisms of progesterone secretion as an intraluteal regulator. Differences observed between the two systems might be due to the presence or absence of cell-to-cell contact. Key words: Oxytocin, Cell-to-cell contact, Corpus luteum, Bovine.(J. Reprod. Dev. 42 : 199-204, 1996) Accepted for publication: June 3, 1996 Correspondence: K. Okuda t is well known that oxytocin (OT) is synthesized and secreted by the corpus luteum (CL) a luteolytic effect [12], or no effect [13,14]. These different results obtained from in vitro studies seem to depend on the experimental model, e.g., shortor long-term culture of luteal cells, cell density and duration of stimulation with reagents. Recently, it has been reported that the communication between large and small luteal cells is very important for the function of the CL [15]. In addition, Miyamoto et al. [16] reported that OT stimulated progesterone production from bovine CL using an in vitro microdialysis system (MDS). In contrast to classical cell culture techniques, MDS maintains cell-to-cell contact as is the case in vivo. Thus, it is strongly suggested that cell-to-cell contact may be a requisite for the CL response to OT.The present study was conducted to investigate I in many species [1][2][3][4][5][6][7]. Moreover, the presence of OT receptors in the CL has been demonstrated in the cow [8], pig [9] and sheep [10]. Although these data suggest that luteal OT plays an important physiological role as an autocrine or paracrine regulator or both, a satisfactory explanation of the physiological role of OT in the CL has not been provided. In vitro models, which are mainly based on the incubation of dispersed luteal cells, have shown a variety of CL responses to OT in terms of progesterone production: a luteotropic effect [11],

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confidence: 99%

Progesterone Release of Bovine Corpus Luteum in Response to Oxytocin in Different Culture Systems.

Sakumoto

Ando

Okuda

1996

Journal of Reproduction and Development

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“…Thus, the weak inhibitory effect of TNF-α at the same dose at luteolysis suggest that this cytokine may be bifunctional throughout the life of cyclic CL (from CL development to CL regression), as has been proposed in the porcine CL [16][17][18].…”

Section: Discussionmentioning

confidence: 97%

Intraluteal Release of Progesterone and Prostaglandins during PGF2.ALPHA.-Induced Luteolysis in Ewes: Local Effects of Tumor Necrosis Factor-.ALPHA..

Miyamoto

Nakatsuka

Ohtani

et al. 1998

Journal of Reproduction and Development

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Abstract. The role of tumor necrosis factor-α (TNF-α) in the local regulatory mechanisms of functional luteolysis in the ewe was examined. TNF-α was infused into a microdialysis system (MDS) implanted in the corpus luteum (CL) for 24 h from 2 h after the intramuscular injection of cloprostenol (defined as 0 h), and plasma as well as intraluteal changes of progesterone (P) and prostaglandins (PG) were observed. Three ewes were treated for superovulation, and multiple CL formed thereafter were surgically implanted with the MDS (1 line/CL) on Day 7 after GnRH injection. Serial fractions from the MDS were collected every 30 min for 40 h on Days 9-11 following a preperfusion period of 30 h. An intramuscular injection of cloprostenol stimulated an acute increase of plasma P concentration (an increase to 150%) within 1 h, followed by a rapid decrease within 6 h (P<0.05), which directly correlates with functional luteolysis. Intraluteal P release into the MDS showed a profile similar to that in the plasma, but significantly decreased from 17 h (P<0.05). Twenty-four-hour infusions of TNF-α at 20 ng/ml and 200 ng/ml into the MDS starting at 2 h after cloprostenol injection slightly hastened the time of onset of P decrease when they were compared with the time of the control. In contrast, the higher dose of TNF-α at 2000 ng/ml clearly delayed the time of onset of P decrease. Intraluteal release of PGF2α and PGE2 in the control was slightly increased between 18-32 h (P<0.05). A 26-h infusion with indomethacin partly blocked the increase in basal release of both PG (P<0.05), whereas it had no effect on the P release. A 24-h infusion with different concentrations of TNF-α stimulated the release of PGE2, but not the release of PGF2α (P<0.05). The results support the idea that TNF-α may have a passive or secondary role in functional luteolysis. Furthermore, the intraluteal PG production appears to have no direct correlation with functional luteolysis.

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“…The binding of oxytocin (OT) to its luteal receptor is involved in the regulation of ovarian steroidogenesis in cyclic sows (Przala et al, 1986;Pitzel et al, 1993bPitzel et al, , 1993c. The concentration of specific oxytocin receptors (OTR) in porcine luteal cells is higher during the early and mid-luteal phases of the oestrous cycle compared to the late luteal phase (Pitzel et al, 1993a).…”

Section: (Received 2 January 2008; Accepted 22 May 2008)mentioning

confidence: 99%

“…An association between activation of the PKC- [Ca 2+ ] i system and luteal secretion of P 4 was observed in pigs (Ciereszko et al, 1998) and ruminants (Niswender, 2002). Oxytocin produced locally in the porcine CL appears to act as a para/autocrine modulator of luteal steroidogenesis (Einspanier et al, 1991;Pitzel et al, 1993bPitzel et al, , 1993c, but no data are available concerning OT signalling in porcine luteal cells. Therefore, in the current study we tested the hypothesis that OT modulates luteal steroidogenesis in pigs by activating the phosphoinositide (PI) pathway.…”

Section: (Received 2 January 2008; Accepted 22 May 2008)mentioning

confidence: 99%

The effect of oxytocin on progesterone secretion, phosphoinositide hydrolysis and intracellular mobilisation of Ca 2+ in porcine luteal cells

Franczak

Kurowicka

Kowalik

et al. 2009

Acta Veterinaria Hungarica

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Oxytocin (OT) is involved in the regulation of steroid secretion by the corpus luteum (CL) in pigs, but OT signal transduction in the porcine CL has not been identified. In this study, the effects of OT on in vitro progesterone (P 4 ) secretion, phosphoinositide (PI) hydrolysis and intracellular mobilisation of Ca 2+ ([Ca 2+ ] i ) were investigated in porcine luteal cells during the early (days 3-5), mid-(days 8-10) and late luteal phases (days 12-14) of the oestrous cycle. Basal concentrations of P 4 and accumulation of inositol phosphates (IPs) were higher (P < 0.05) on days 3-5 and 8-10 of the oestrous cycle than on days 12-14. Basal [Ca 2+ ] i mobilisation did not differ among studied periods of the oestrous cycle. Oxytocin (10 -7 M) enhanced P 4 secretion and PI hydrolysis (P < 0.05) by luteal cells harvested on days 8-10 of the oestrous cycle. Moreover, OT started to increase mobilisation of [Ca 2+ ] i at the 15th (days 3-5 and 8-10) or 30th second (days 12-14) in porcine luteal cells. It was concluded that in pigs OT acts as a regulator of steroidogenesis, stimulating P 4 secretion in mature CL. This OT action may be mediated by changes in PI hydrolysis and [Ca 2+ ] i mobilisation.

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Effects and interactions of prostaglandin F2 alpha, oxytocin, and cytokines on steroidogenesis of porcine luteal cells

Cited by 39 publications

References 0 publications

Progesterone Release of Bovine Corpus Luteum in Response to Oxytocin in Different Culture Systems.

Progesterone Release of Bovine Corpus Luteum in Response to Oxytocin in Different Culture Systems.

Intraluteal Release of Progesterone and Prostaglandins during PGF2.ALPHA.-Induced Luteolysis in Ewes: Local Effects of Tumor Necrosis Factor-.ALPHA..

The effect of oxytocin on progesterone secretion, phosphoinositide hydrolysis and intracellular mobilisation of Ca 2+ in porcine luteal cells

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