Effectivity of Lactobacillus plantarum BSL against Listeria monocytogenes in rats

Abstract

Aims: To evaluate the effectivity of Lactobacillus plantarum BSL isolated from Indonesian sauerkraut against Listeria monocytogenes ATCC 7644 through in vitro and in vivo assay. Methodology and results: In vitro examination for antimicrobial activity against L. monocytogenes ATCC 7644 was performed using seven isolates of lactic acid bacteria (LAB). Lactobacillus plantarum BSL demonstrated the highest activity against L. monocytogenes and studied further in Sprague-Dawley (SD) rats. Treatment group of rats received 0.5 mL culture suspension (10 9 CFU/mL) of L. plantarum BSL and control group received 0.5 mL of 0.85% w/v NaCl daily during nine days of treatment. Both groups were infected at 3rd day with 0.5 mL of suspension of L. monocytogenes (10 9 CFU/mL). At the 2 nd (before infection), 5 th , 7 th , and 9 th day (after infection), the rats were sacrificed and the faeces, caecum, and caecum content were examined for the population of LAB and L. monocytogenes. Administration of L. plantarum BSL significantly increased the population of LAB by 1.2-1.4 log unit, while the number of L. monocytogenes was reduced by 1.8-1.9 log unit compared to control group eithr in the faeces, caecum, or caecum content. Administration of L. plantarum BSL could be able to reduce the liver and spleen damage of the experimental rats, but did not show any changes in immunoglobulin A (IgA) response in comparison with control group. Conclusion, significance and impact of study: Lactobacillus plantarum BSL was promising as probiotic candidate with health promotion to protect the gastrointestinal from infection by L. monocytogenes ATCC 7644.

THE ABILITY OF Lactobacillus plantarum BSL IN REDUCING THE TISSUE DAMAGE OF LIVER AND SPLEEN IN RATS INFECTED BY Listeria monocytogenes ATCC 7644

“…Other than that, L. plantarum B7 was able to reduce liver translocation and ameliorates histopathological lesions in rats infected by S. thphimurium (Tsai et al 2005), and then Acurcio et al (2017) also reported that administration of L. plantarum B7 significantly extent of ileal villi heights and number of hepatic inflammatory foci significantly lower than the control group. In addition, Meiyasa (2017) reported that treatment with L. plantarum BSL during nine-days could be able to reduce damage of liver and spleen in rats such as edema, necrosis, cell infiltration of lymphoid, proliferation of bile tract, vacuolization of hepatocytes cell for liver and depletion of lymphoid cells, extension of pulp area, and cell proliferation for spleen. Administration of L. plantarum BSL can reduce tissue damage of liver and spleen in rats possibly through mechanisms such as the production of antagonistic compounds and inhibitory substances, protective effects against pathogens (Acurcio et al 2017;Servin 2004;Prieto et al 2015 competition for nutrients or adhesion sites and/or immunomodulation (Bambirra et al 2007;Gibson et al 2005), cytokine production (Castanheira et al 2007), and reduces pro-inflammatory interleukin secretion (Puertollano et al 2008).…”

“…L. plantarum BSL was grown in MRS broth (Oxoid CM359) at 37 °C for 24 h, followed by pour plating in MRS agar (Oxoid CM361) incubated at 37 °C for 24-48 h for counting the bacterial cells. L. monocytogenes strain ATCC 7644 was cultured BHI broth (Oxoid CM1136) incubated at 37 °C for 24 h, and then counted in Listeria Selective Agar Base (Oxoid CM856), after incubation at 37 °C for 24-48 h (Meiyasa et al 2018).…”

“…The number of cells of L. plantarum BSL in suspension was enumerated in MRS agar. Similar method was applied for L. monocytogenes ATCC 7644, only the media was used BHI broth (Meiyasa et al 2018).…”

“…Moreover, L. plantarum BSL were able to reduce total cholesterol of rat blood serum, enhance lactobacilli growth, and suppress coliform and staphylococci in rat faeces (Kusumawati et al 2003;Kusumawati et al 2008). In addition, Meiyasa et al (2017) reported that administration of L. plantarum BSL significantly increased the population of LAB and reduced the number of L. monocytogenes compared to control group either in the faeces, caecum, or caecum content, and it effectively reduced the liver and spleen tissues damage in rats. Numerous studies have reported that probiotics have a beneficial effect on the host.…”

Evaluation of single and multispecies probiotic applications for the prevention of Motile Aeromonads Septicaemia in gourami, Osphronemus gourami

“…Lactobacillus plantarum has capability in producing amylase, protease, and lipase enzymes as growth promoters (Sulistiani, 2017). L. plantarum bacteria can also produce lactic acid which modulates the immune responses, and produces plantaricin as an antimicrobial compound that can kill bacteria by disrupting the protein synthesis of pathogenic bacteria (Meiyasa et al, 2017). The demonstrations of many beneficial aspects produced by probiotic bacteria of Bacillus and L. plantarum and further combination of mixed cultures of both are expected to synergize with each other.…”